Orenia metallireducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halobacteroidaceae; Orenia

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C0A7R3|A0A1C0A7R3_9FIRM Yip1 domain-containing protein OS=Orenia metallireducens OX=1413210 GN=U472_09710 PE=4 SV=1
MM1 pKa = 7.35SVNIIYY7 pKa = 10.42NAINVNSLNTNSTVSIGEE25 pKa = 4.04NAQTNWDD32 pKa = 3.72SHH34 pKa = 5.34NKK36 pKa = 8.9NNYY39 pKa = 9.44GNGSHH44 pKa = 6.27YY45 pKa = 11.02GIVNVLAPSNIIFDD59 pKa = 4.17NDD61 pKa = 3.69ILDD64 pKa = 4.0TPINDD69 pKa = 3.86PDD71 pKa = 4.06FVPTAQAEE79 pKa = 4.21

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C0ACY8|A0A1C0ACY8_9FIRM Bifunctional purine biosynthesis protein PurH OS=Orenia metallireducens OX=1413210 GN=purH PE=3 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84GRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 10.39SCDD10 pKa = 2.96FCANKK15 pKa = 9.64VDD17 pKa = 3.99KK18 pKa = 10.57IDD20 pKa = 3.6YY21 pKa = 10.2KK22 pKa = 10.86RR23 pKa = 11.84INILRR28 pKa = 11.84KK29 pKa = 9.85YY30 pKa = 8.46ITDD33 pKa = 3.42RR34 pKa = 11.84GKK36 pKa = 9.9ILPRR40 pKa = 11.84RR41 pKa = 11.84ISGNCAKK48 pKa = 10.16HH49 pKa = 5.2QRR51 pKa = 11.84EE52 pKa = 4.23LTSAIKK58 pKa = 10.17RR59 pKa = 11.84ARR61 pKa = 11.84NIALLPYY68 pKa = 10.24KK69 pKa = 10.1IDD71 pKa = 3.32

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3070

0

3070

907535

37

2967

295.6

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.169 ± 0.055

0.717 ± 0.016

5.922 ± 0.046

8.222 ± 0.052

4.04 ± 0.039

6.733 ± 0.046

1.448 ± 0.017

9.222 ± 0.05

8.116 ± 0.05

10.156 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.021

5.368 ± 0.04

2.966 ± 0.027

3.036 ± 0.024

3.936 ± 0.029

5.772 ± 0.033

4.66 ± 0.033

6.654 ± 0.037

0.774 ± 0.016

3.855 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski