Amino acid dipepetide frequency for Nostoc sp. NIES-4103

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.404AlaAla: 7.404 ± 0.084
0.808AlaCys: 0.808 ± 0.021
3.965AlaAsp: 3.965 ± 0.045
5.006AlaGlu: 5.006 ± 0.058
3.01AlaPhe: 3.01 ± 0.043
5.448AlaGly: 5.448 ± 0.077
1.263AlaHis: 1.263 ± 0.024
6.778AlaIle: 6.778 ± 0.074
4.244AlaLys: 4.244 ± 0.05
8.331AlaLeu: 8.331 ± 0.067
1.52AlaMet: 1.52 ± 0.026
3.621AlaAsn: 3.621 ± 0.059
2.746AlaPro: 2.746 ± 0.042
4.132AlaGln: 4.132 ± 0.058
3.323AlaArg: 3.323 ± 0.046
4.869AlaSer: 4.869 ± 0.058
4.797AlaThr: 4.797 ± 0.058
5.529AlaVal: 5.529 ± 0.061
1.016AlaTrp: 1.016 ± 0.023
2.411AlaTyr: 2.411 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.639CysAla: 0.639 ± 0.019
0.165CysCys: 0.165 ± 0.008
0.573CysAsp: 0.573 ± 0.018
0.537CysGlu: 0.537 ± 0.015
0.436CysPhe: 0.436 ± 0.014
0.756CysGly: 0.756 ± 0.018
0.277CysHis: 0.277 ± 0.012
0.612CysIle: 0.612 ± 0.017
0.361CysLys: 0.361 ± 0.013
1.133CysLeu: 1.133 ± 0.028
0.154CysMet: 0.154 ± 0.01
0.4CysAsn: 0.4 ± 0.013
0.541CysPro: 0.541 ± 0.017
0.575CysGln: 0.575 ± 0.017
0.498CysArg: 0.498 ± 0.016
0.594CysSer: 0.594 ± 0.018
0.466CysThr: 0.466 ± 0.013
0.581CysVal: 0.581 ± 0.017
0.141CysTrp: 0.141 ± 0.008
0.351CysTyr: 0.351 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.608AspAla: 3.608 ± 0.044
0.486AspCys: 0.486 ± 0.015
2.249AspAsp: 2.249 ± 0.048
3.042AspGlu: 3.042 ± 0.046
2.33AspPhe: 2.33 ± 0.035
3.563AspGly: 3.563 ± 0.078
0.737AspHis: 0.737 ± 0.02
3.591AspIle: 3.591 ± 0.05
2.275AspLys: 2.275 ± 0.036
5.446AspLeu: 5.446 ± 0.065
0.727AspMet: 0.727 ± 0.016
2.174AspAsn: 2.174 ± 0.039
2.2AspPro: 2.2 ± 0.041
1.835AspGln: 1.835 ± 0.035
2.896AspArg: 2.896 ± 0.043
3.075AspSer: 3.075 ± 0.049
2.616AspThr: 2.616 ± 0.049
3.054AspVal: 3.054 ± 0.038
0.929AspTrp: 0.929 ± 0.021
1.993AspTyr: 1.993 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.174GluAla: 5.174 ± 0.054
0.494GluCys: 0.494 ± 0.019
2.655GluAsp: 2.655 ± 0.037
3.855GluGlu: 3.855 ± 0.048
2.449GluPhe: 2.449 ± 0.034
3.037GluGly: 3.037 ± 0.04
1.009GluHis: 1.009 ± 0.023
4.952GluIle: 4.952 ± 0.055
3.488GluLys: 3.488 ± 0.052
6.971GluLeu: 6.971 ± 0.074
1.231GluMet: 1.231 ± 0.025
2.848GluAsn: 2.848 ± 0.035
2.341GluPro: 2.341 ± 0.036
3.687GluGln: 3.687 ± 0.041
3.264GluArg: 3.264 ± 0.042
3.345GluSer: 3.345 ± 0.041
3.299GluThr: 3.299 ± 0.038
4.315GluVal: 4.315 ± 0.046
0.786GluTrp: 0.786 ± 0.02
2.063GluTyr: 2.063 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.175PheAla: 3.175 ± 0.041
0.524PheCys: 0.524 ± 0.016
2.217PheAsp: 2.217 ± 0.035
2.184PheGlu: 2.184 ± 0.03
1.709PhePhe: 1.709 ± 0.033
2.878PheGly: 2.878 ± 0.049
0.781PheHis: 0.781 ± 0.02
2.45PheIle: 2.45 ± 0.035
1.631PheLys: 1.631 ± 0.03
4.082PheLeu: 4.082 ± 0.051
0.661PheMet: 0.661 ± 0.018
1.889PheAsn: 1.889 ± 0.033
1.781PhePro: 1.781 ± 0.031
1.904PheGln: 1.904 ± 0.034
1.76PheArg: 1.76 ± 0.024
3.061PheSer: 3.061 ± 0.049
2.401PheThr: 2.401 ± 0.039
2.533PheVal: 2.533 ± 0.033
0.676PheTrp: 0.676 ± 0.017
1.424PheTyr: 1.424 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
4.598GlyAla: 4.598 ± 0.066
0.776GlyCys: 0.776 ± 0.021
3.444GlyAsp: 3.444 ± 0.075
3.996GlyGlu: 3.996 ± 0.046
2.931GlyPhe: 2.931 ± 0.054
4.748GlyGly: 4.748 ± 0.098
1.282GlyHis: 1.282 ± 0.036
5.11GlyIle: 5.11 ± 0.052
4.017GlyLys: 4.017 ± 0.053
6.664GlyLeu: 6.664 ± 0.066
1.348GlyMet: 1.348 ± 0.027
3.403GlyAsn: 3.403 ± 0.097
1.33GlyPro: 1.33 ± 0.028
2.878GlyGln: 2.878 ± 0.041
3.173GlyArg: 3.173 ± 0.041
4.224GlySer: 4.224 ± 0.064
3.899GlyThr: 3.899 ± 0.07
4.682GlyVal: 4.682 ± 0.057
1.089GlyTrp: 1.089 ± 0.024
2.44GlyTyr: 2.44 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
1.085HisAla: 1.085 ± 0.024
0.261HisCys: 0.261 ± 0.011
0.768HisAsp: 0.768 ± 0.022
1.005HisGlu: 1.005 ± 0.019
0.82HisPhe: 0.82 ± 0.022
1.116HisGly: 1.116 ± 0.025
0.604HisHis: 0.604 ± 0.021
1.15HisIle: 1.15 ± 0.023
0.816HisLys: 0.816 ± 0.019
2.246HisLeu: 2.246 ± 0.03
0.259HisMet: 0.259 ± 0.012
0.802HisAsn: 0.802 ± 0.02
1.315HisPro: 1.315 ± 0.028
1.2HisGln: 1.2 ± 0.029
1.047HisArg: 1.047 ± 0.023
1.241HisSer: 1.241 ± 0.028
0.984HisThr: 0.984 ± 0.026
0.876HisVal: 0.876 ± 0.023
0.357HisTrp: 0.357 ± 0.013
0.683HisTyr: 0.683 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.152IleAla: 7.152 ± 0.065
0.724IleCys: 0.724 ± 0.02
3.707IleAsp: 3.707 ± 0.042
4.313IleGlu: 4.313 ± 0.049
2.634IlePhe: 2.634 ± 0.036
4.497IleGly: 4.497 ± 0.048
1.269IleHis: 1.269 ± 0.022
3.96IleIle: 3.96 ± 0.051
3.322IleLys: 3.322 ± 0.044
6.89IleLeu: 6.89 ± 0.062
0.856IleMet: 0.856 ± 0.018
3.369IleAsn: 3.369 ± 0.05
3.642IlePro: 3.642 ± 0.045
3.189IleGln: 3.189 ± 0.039
3.078IleArg: 3.078 ± 0.038
4.521IleSer: 4.521 ± 0.053
3.996IleThr: 3.996 ± 0.053
4.351IleVal: 4.351 ± 0.05
0.915IleTrp: 0.915 ± 0.021
2.087IleTyr: 2.087 ± 0.04
0.001IleXaa: 0.001 ± 0.002
Lys
4.085LysAla: 4.085 ± 0.053
0.324LysCys: 0.324 ± 0.012
2.238LysAsp: 2.238 ± 0.043
2.783LysGlu: 2.783 ± 0.044
1.85LysPhe: 1.85 ± 0.033
2.659LysGly: 2.659 ± 0.036
0.855LysHis: 0.855 ± 0.024
3.673LysIle: 3.673 ± 0.044
2.361LysLys: 2.361 ± 0.035
5.693LysLeu: 5.693 ± 0.057
0.905LysMet: 0.905 ± 0.021
2.3LysAsn: 2.3 ± 0.037
2.528LysPro: 2.528 ± 0.041
2.974LysGln: 2.974 ± 0.044
2.302LysArg: 2.302 ± 0.031
3.04LysSer: 3.04 ± 0.041
2.996LysThr: 2.996 ± 0.042
3.259LysVal: 3.259 ± 0.033
0.506LysTrp: 0.506 ± 0.014
1.576LysTyr: 1.576 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
9.51LeuAla: 9.51 ± 0.079
1.058LeuCys: 1.058 ± 0.023
5.167LeuAsp: 5.167 ± 0.059
7.231LeuGlu: 7.231 ± 0.081
3.743LeuPhe: 3.743 ± 0.053
7.423LeuGly: 7.423 ± 0.07
1.993LeuHis: 1.993 ± 0.033
6.874LeuIle: 6.874 ± 0.06
5.552LeuLys: 5.552 ± 0.057
11.431LeuLeu: 11.431 ± 0.108
2.024LeuMet: 2.024 ± 0.036
4.815LeuAsn: 4.815 ± 0.049
5.591LeuPro: 5.591 ± 0.059
6.035LeuGln: 6.035 ± 0.063
5.458LeuArg: 5.458 ± 0.054
7.434LeuSer: 7.434 ± 0.067
6.526LeuThr: 6.526 ± 0.058
7.039LeuVal: 7.039 ± 0.056
1.485LeuTrp: 1.485 ± 0.038
2.821LeuTyr: 2.821 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
1.58MetAla: 1.58 ± 0.028
0.138MetCys: 0.138 ± 0.008
0.668MetAsp: 0.668 ± 0.018
0.911MetGlu: 0.911 ± 0.021
0.54MetPhe: 0.54 ± 0.016
1.216MetGly: 1.216 ± 0.027
0.325MetHis: 0.325 ± 0.013
1.051MetIle: 1.051 ± 0.021
0.917MetLys: 0.917 ± 0.021
1.868MetLeu: 1.868 ± 0.034
0.382MetMet: 0.382 ± 0.015
0.836MetAsn: 0.836 ± 0.019
0.884MetPro: 0.884 ± 0.019
0.944MetGln: 0.944 ± 0.021
0.896MetArg: 0.896 ± 0.022
1.26MetSer: 1.26 ± 0.023
1.216MetThr: 1.216 ± 0.024
1.163MetVal: 1.163 ± 0.025
0.164MetTrp: 0.164 ± 0.009
0.374MetTyr: 0.374 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.065AsnAla: 3.065 ± 0.047
0.476AsnCys: 0.476 ± 0.015
1.945AsnAsp: 1.945 ± 0.062
1.951AsnGlu: 1.951 ± 0.034
2.09AsnPhe: 2.09 ± 0.032
3.004AsnGly: 3.004 ± 0.08
0.916AsnHis: 0.916 ± 0.023
3.056AsnIle: 3.056 ± 0.041
1.883AsnLys: 1.883 ± 0.031
5.709AsnLeu: 5.709 ± 0.077
0.639AsnMet: 0.639 ± 0.018
2.364AsnAsn: 2.364 ± 0.052
2.885AsnPro: 2.885 ± 0.048
2.874AsnGln: 2.874 ± 0.037
2.266AsnArg: 2.266 ± 0.03
3.403AsnSer: 3.403 ± 0.05
2.601AsnThr: 2.601 ± 0.043
2.342AsnVal: 2.342 ± 0.042
0.8AsnTrp: 0.8 ± 0.021
1.766AsnTyr: 1.766 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
3.092ProAla: 3.092 ± 0.048
0.344ProCys: 0.344 ± 0.014
2.976ProAsp: 2.976 ± 0.04
3.632ProGlu: 3.632 ± 0.041
1.738ProPhe: 1.738 ± 0.027
2.904ProGly: 2.904 ± 0.046
0.974ProHis: 0.974 ± 0.021
3.061ProIle: 3.061 ± 0.04
2.252ProLys: 2.252 ± 0.033
4.567ProLeu: 4.567 ± 0.051
0.698ProMet: 0.698 ± 0.015
2.391ProAsn: 2.391 ± 0.043
2.248ProPro: 2.248 ± 0.04
2.74ProGln: 2.74 ± 0.043
1.667ProArg: 1.667 ± 0.03
3.048ProSer: 3.048 ± 0.048
2.935ProThr: 2.935 ± 0.045
3.213ProVal: 3.213 ± 0.045
0.571ProTrp: 0.571 ± 0.016
1.392ProTyr: 1.392 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
5.005GlnAla: 5.005 ± 0.055
0.399GlnCys: 0.399 ± 0.015
2.199GlnAsp: 2.199 ± 0.029
3.693GlnGlu: 3.693 ± 0.044
1.796GlnPhe: 1.796 ± 0.03
3.283GlnGly: 3.283 ± 0.039
0.932GlnHis: 0.932 ± 0.022
3.829GlnIle: 3.829 ± 0.048
2.96GlnLys: 2.96 ± 0.038
6.249GlnLeu: 6.249 ± 0.07
1.031GlnMet: 1.031 ± 0.022
2.237GlnAsn: 2.237 ± 0.036
2.714GlnPro: 2.714 ± 0.04
4.223GlnGln: 4.223 ± 0.068
2.924GlnArg: 2.924 ± 0.044
2.978GlnSer: 2.978 ± 0.046
3.136GlnThr: 3.136 ± 0.046
3.844GlnVal: 3.844 ± 0.047
0.683GlnTrp: 0.683 ± 0.018
1.403GlnTyr: 1.403 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
3.046ArgAla: 3.046 ± 0.042
0.481ArgCys: 0.481 ± 0.013
2.357ArgAsp: 2.357 ± 0.034
3.05ArgGlu: 3.05 ± 0.04
2.141ArgPhe: 2.141 ± 0.03
2.841ArgGly: 2.841 ± 0.039
0.976ArgHis: 0.976 ± 0.019
3.179ArgIle: 3.179 ± 0.044
2.166ArgLys: 2.166 ± 0.037
5.792ArgLeu: 5.792 ± 0.062
0.929ArgMet: 0.929 ± 0.022
2.058ArgAsn: 2.058 ± 0.032
1.949ArgPro: 1.949 ± 0.031
3.225ArgGln: 3.225 ± 0.048
2.838ArgArg: 2.838 ± 0.045
3.074ArgSer: 3.074 ± 0.044
2.469ArgThr: 2.469 ± 0.034
3.314ArgVal: 3.314 ± 0.04
0.834ArgTrp: 0.834 ± 0.022
1.906ArgTyr: 1.906 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
4.462SerAla: 4.462 ± 0.049
0.626SerCys: 0.626 ± 0.017
3.299SerAsp: 3.299 ± 0.043
3.628SerGlu: 3.628 ± 0.042
2.657SerPhe: 2.657 ± 0.037
4.685SerGly: 4.685 ± 0.068
1.337SerHis: 1.337 ± 0.027
3.927SerIle: 3.927 ± 0.049
2.751SerLys: 2.751 ± 0.032
7.473SerLeu: 7.473 ± 0.072
1.127SerMet: 1.127 ± 0.022
3.019SerAsn: 3.019 ± 0.05
3.557SerPro: 3.557 ± 0.061
3.856SerGln: 3.856 ± 0.051
3.056SerArg: 3.056 ± 0.037
4.733SerSer: 4.733 ± 0.069
3.634SerThr: 3.634 ± 0.05
4.089SerVal: 4.089 ± 0.048
0.948SerTrp: 0.948 ± 0.021
1.994SerTyr: 1.994 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
4.895ThrAla: 4.895 ± 0.064
0.479ThrCys: 0.479 ± 0.015
2.743ThrAsp: 2.743 ± 0.044
3.404ThrGlu: 3.404 ± 0.051
2.225ThrPhe: 2.225 ± 0.037
4.419ThrGly: 4.419 ± 0.072
1.015ThrHis: 1.015 ± 0.024
3.898ThrIle: 3.898 ± 0.051
2.539ThrLys: 2.539 ± 0.037
6.384ThrLeu: 6.384 ± 0.063
0.76ThrMet: 0.76 ± 0.018
2.513ThrAsn: 2.513 ± 0.041
3.382ThrPro: 3.382 ± 0.051
3.015ThrGln: 3.015 ± 0.048
2.321ThrArg: 2.321 ± 0.032
3.712ThrSer: 3.712 ± 0.048
3.655ThrThr: 3.655 ± 0.06
4.107ThrVal: 4.107 ± 0.051
0.722ThrTrp: 0.722 ± 0.021
1.725ThrTyr: 1.725 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
5.678ValAla: 5.678 ± 0.063
0.658ValCys: 0.658 ± 0.018
3.426ValAsp: 3.426 ± 0.041
4.352ValGlu: 4.352 ± 0.054
2.608ValPhe: 2.608 ± 0.043
4.425ValGly: 4.425 ± 0.053
1.024ValHis: 1.024 ± 0.025
4.524ValIle: 4.524 ± 0.047
3.375ValLys: 3.375 ± 0.044
6.638ValLeu: 6.638 ± 0.061
1.324ValMet: 1.324 ± 0.027
3.008ValAsn: 3.008 ± 0.042
2.831ValPro: 2.831 ± 0.039
2.947ValGln: 2.947 ± 0.039
3.147ValArg: 3.147 ± 0.047
4.279ValSer: 4.279 ± 0.047
3.924ValThr: 3.924 ± 0.052
4.911ValVal: 4.911 ± 0.055
0.888ValTrp: 0.888 ± 0.022
1.909ValTyr: 1.909 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
0.845TrpAla: 0.845 ± 0.021
0.162TrpCys: 0.162 ± 0.009
0.689TrpAsp: 0.689 ± 0.026
0.89TrpGlu: 0.89 ± 0.021
0.596TrpPhe: 0.596 ± 0.016
0.984TrpGly: 0.984 ± 0.024
0.35TrpHis: 0.35 ± 0.014
0.863TrpIle: 0.863 ± 0.021
0.617TrpLys: 0.617 ± 0.019
1.909TrpLeu: 1.909 ± 0.038
0.3TrpMet: 0.3 ± 0.013
0.67TrpAsn: 0.67 ± 0.022
0.289TrpPro: 0.289 ± 0.014
1.216TrpGln: 1.216 ± 0.029
0.823TrpArg: 0.823 ± 0.021
0.874TrpSer: 0.874 ± 0.024
0.618TrpThr: 0.618 ± 0.018
0.966TrpVal: 0.966 ± 0.023
0.239TrpTrp: 0.239 ± 0.012
0.444TrpTyr: 0.444 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.125TyrAla: 2.125 ± 0.034
0.407TyrCys: 0.407 ± 0.015
1.543TyrAsp: 1.543 ± 0.041
1.8TyrGlu: 1.8 ± 0.031
1.418TyrPhe: 1.418 ± 0.029
2.145TyrGly: 2.145 ± 0.04
0.716TyrHis: 0.716 ± 0.02
1.935TyrIle: 1.935 ± 0.034
1.381TyrLys: 1.381 ± 0.023
3.734TyrLeu: 3.734 ± 0.042
0.455TyrMet: 0.455 ± 0.013
1.321TyrAsn: 1.321 ± 0.031
1.688TyrPro: 1.688 ± 0.029
2.068TyrGln: 2.068 ± 0.034
1.89TyrArg: 1.89 ± 0.034
2.091TyrSer: 2.091 ± 0.038
1.743TyrThr: 1.743 ± 0.035
1.709TyrVal: 1.709 ± 0.031
0.592TyrTrp: 0.592 ± 0.017
1.213TyrTyr: 1.213 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.002
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6898 proteins (2231661 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski