Synechococcus phage S-RSM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Cymopoleiavirus; unclassified Cymopoleiavirus

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7BUY8|C7BUY8_9CAUD Photosystem II OS=Synechococcus phage S-RSM4 OX=555387 GN=psbD PE=3 SV=1
MM1 pKa = 7.2AQATVLYY8 pKa = 9.56FSATVSFIFLCVGVIAGWTANEE30 pKa = 4.01KK31 pKa = 10.33LHH33 pKa = 6.08EE34 pKa = 4.23FMYY37 pKa = 10.76GKK39 pKa = 10.1AQEE42 pKa = 4.4EE43 pKa = 4.51NIHH46 pKa = 6.71PEE48 pKa = 3.91MLDD51 pKa = 5.51GEE53 pKa = 4.67GQWINEE59 pKa = 4.03EE60 pKa = 4.08LLSVRR65 pKa = 11.84FVDD68 pKa = 5.73EE69 pKa = 6.2DD70 pKa = 4.53DD71 pKa = 5.82DD72 pKa = 7.0DD73 pKa = 6.54YY74 pKa = 12.18DD75 pKa = 4.35DD76 pKa = 4.26

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7BV11|C7BV11_9CAUD Hypothetical cyanophage protein OS=Synechococcus phage S-RSM4 OX=555387 GN=SRSM4_043 PE=4 SV=1
MM1 pKa = 7.62GFTTPRR7 pKa = 11.84VSYY10 pKa = 9.43FLKK13 pKa = 10.94VSGADD18 pKa = 3.27GNTPAWFLASSQRR31 pKa = 11.84ASGGSKK37 pKa = 10.27

Molecular weight: 3.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

60981

37

4482

257.3

28.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.884 ± 0.22

0.841 ± 0.074

6.878 ± 0.137

6.248 ± 0.231

4.408 ± 0.104

8.142 ± 0.405

1.469 ± 0.112

6.235 ± 0.15

5.426 ± 0.297

7.071 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.871 ± 0.174

5.912 ± 0.151

4.023 ± 0.097

3.68 ± 0.111

4.101 ± 0.132

6.956 ± 0.238

7.422 ± 0.316

6.753 ± 0.178

1.194 ± 0.084

4.487 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski