Amino acid dipepetide frequency for Leptolyngbya sp. Heron Island J

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.017AlaAla: 9.017 ± 0.08	0.862AlaCys: 0.862 ± 0.022	5.195AlaAsp: 5.195 ± 0.046	5.512AlaGlu: 5.512 ± 0.058	3.086AlaPhe: 3.086 ± 0.036	5.883AlaGly: 5.883 ± 0.068	1.616AlaHis: 1.616 ± 0.033	6.581AlaIle: 6.581 ± 0.063	3.474AlaLys: 3.474 ± 0.047	9.928AlaLeu: 9.928 ± 0.077	2.132AlaMet: 2.132 ± 0.034	3.488AlaAsn: 3.488 ± 0.051	3.459AlaPro: 3.459 ± 0.049	4.842AlaGln: 4.842 ± 0.059	4.073AlaArg: 4.073 ± 0.043	5.201AlaSer: 5.201 ± 0.05	5.348AlaThr: 5.348 ± 0.059	6.225AlaVal: 6.225 ± 0.051	1.138AlaTrp: 1.138 ± 0.025	2.572AlaTyr: 2.572 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.653CysAla: 0.653 ± 0.017	0.173CysCys: 0.173 ± 0.009	0.676CysAsp: 0.676 ± 0.016	0.474CysGlu: 0.474 ± 0.014	0.419CysPhe: 0.419 ± 0.015	0.733CysGly: 0.733 ± 0.019	0.295CysHis: 0.295 ± 0.013	0.522CysIle: 0.522 ± 0.013	0.286CysLys: 0.286 ± 0.011	1.21CysLeu: 1.21 ± 0.026	0.165CysMet: 0.165 ± 0.009	0.334CysAsn: 0.334 ± 0.014	0.521CysPro: 0.521 ± 0.018	0.57CysGln: 0.57 ± 0.018	0.57CysArg: 0.57 ± 0.016	0.605CysSer: 0.605 ± 0.016	0.466CysThr: 0.466 ± 0.015	0.576CysVal: 0.576 ± 0.017	0.156CysTrp: 0.156 ± 0.009	0.319CysTyr: 0.319 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	4.528AspAla: 4.528 ± 0.056	0.56AspCys: 0.56 ± 0.019	3.16AspAsp: 3.16 ± 0.044	3.247AspGlu: 3.247 ± 0.043	2.373AspPhe: 2.373 ± 0.037	3.959AspGly: 3.959 ± 0.066	1.096AspHis: 1.096 ± 0.023	3.622AspIle: 3.622 ± 0.043	1.649AspLys: 1.649 ± 0.033	6.126AspLeu: 6.126 ± 0.063	0.997AspMet: 0.997 ± 0.023	2.058AspAsn: 2.058 ± 0.034	2.692AspPro: 2.692 ± 0.037	2.657AspGln: 2.657 ± 0.037	3.282AspArg: 3.282 ± 0.038	3.216AspSer: 3.216 ± 0.042	2.948AspThr: 2.948 ± 0.043	3.719AspVal: 3.719 ± 0.047	1.019AspTrp: 1.019 ± 0.022	2.048AspTyr: 2.048 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	5.601GluAla: 5.601 ± 0.066	0.404GluCys: 0.404 ± 0.015	2.818GluAsp: 2.818 ± 0.039	3.191GluGlu: 3.191 ± 0.046	2.175GluPhe: 2.175 ± 0.031	3.205GluGly: 3.205 ± 0.047	1.183GluHis: 1.183 ± 0.024	3.896GluIle: 3.896 ± 0.047	2.391GluLys: 2.391 ± 0.036	6.713GluLeu: 6.713 ± 0.065	1.248GluMet: 1.248 ± 0.023	2.193GluAsn: 2.193 ± 0.029	2.714GluPro: 2.714 ± 0.04	3.726GluGln: 3.726 ± 0.051	3.664GluArg: 3.664 ± 0.046	3.292GluSer: 3.292 ± 0.043	3.935GluThr: 3.935 ± 0.043	3.827GluVal: 3.827 ± 0.041	0.707GluTrp: 0.707 ± 0.018	1.397GluTyr: 1.397 ± 0.031	0.0GluXaa: 0.0 ± 0.001
Phe	3.047PheAla: 3.047 ± 0.042	0.485PheCys: 0.485 ± 0.015	2.442PheAsp: 2.442 ± 0.035	2.344PheGlu: 2.344 ± 0.035	1.602PhePhe: 1.602 ± 0.033	2.927PheGly: 2.927 ± 0.039	0.737PheHis: 0.737 ± 0.019	2.076PheIle: 2.076 ± 0.034	1.381PheLys: 1.381 ± 0.025	3.888PheLeu: 3.888 ± 0.046	0.735PheMet: 0.735 ± 0.018	1.614PheAsn: 1.614 ± 0.032	1.629PhePro: 1.629 ± 0.031	1.712PheGln: 1.712 ± 0.027	1.837PheArg: 1.837 ± 0.029	3.017PheSer: 3.017 ± 0.045	2.214PheThr: 2.214 ± 0.033	2.436PheVal: 2.436 ± 0.039	0.686PheTrp: 0.686 ± 0.017	1.27PheTyr: 1.27 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	5.383GlyAla: 5.383 ± 0.072	0.803GlyCys: 0.803 ± 0.02	3.77GlyAsp: 3.77 ± 0.063	3.876GlyGlu: 3.876 ± 0.045	3.114GlyPhe: 3.114 ± 0.04	4.903GlyGly: 4.903 ± 0.086	1.564GlyHis: 1.564 ± 0.029	4.648GlyIle: 4.648 ± 0.058	2.845GlyLys: 2.845 ± 0.035	7.831GlyLeu: 7.831 ± 0.079	1.608GlyMet: 1.608 ± 0.029	2.766GlyAsn: 2.766 ± 0.064	2.068GlyPro: 2.068 ± 0.034	3.626GlyGln: 3.626 ± 0.045	3.535GlyArg: 3.535 ± 0.048	4.109GlySer: 4.109 ± 0.046	3.951GlyThr: 3.951 ± 0.056	4.735GlyVal: 4.735 ± 0.049	1.189GlyTrp: 1.189 ± 0.026	2.434GlyTyr: 2.434 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	1.307HisAla: 1.307 ± 0.028	0.305HisCys: 0.305 ± 0.013	0.944HisAsp: 0.944 ± 0.023	0.997HisGlu: 0.997 ± 0.023	0.851HisPhe: 0.851 ± 0.02	1.438HisGly: 1.438 ± 0.031	0.761HisHis: 0.761 ± 0.023	1.157HisIle: 1.157 ± 0.024	0.618HisLys: 0.618 ± 0.016	2.447HisLeu: 2.447 ± 0.038	0.353HisMet: 0.353 ± 0.012	0.712HisAsn: 0.712 ± 0.017	1.4HisPro: 1.4 ± 0.027	1.409HisGln: 1.409 ± 0.028	1.368HisArg: 1.368 ± 0.025	1.22HisSer: 1.22 ± 0.027	1.075HisThr: 1.075 ± 0.024	1.096HisVal: 1.096 ± 0.024	0.418HisTrp: 0.418 ± 0.013	0.752HisTyr: 0.752 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.52IleAla: 6.52 ± 0.07	0.664IleCys: 0.664 ± 0.017	3.766IleAsp: 3.766 ± 0.04	4.046IleGlu: 4.046 ± 0.043	2.188IlePhe: 2.188 ± 0.032	4.203IleGly: 4.203 ± 0.05	1.185IleHis: 1.185 ± 0.024	2.864IleIle: 2.864 ± 0.041	2.099IleLys: 2.099 ± 0.036	5.916IleLeu: 5.916 ± 0.052	0.856IleMet: 0.856 ± 0.019	2.38IleAsn: 2.38 ± 0.037	3.032IlePro: 3.032 ± 0.039	2.844IleGln: 2.844 ± 0.04	3.138IleArg: 3.138 ± 0.042	4.136IleSer: 4.136 ± 0.041	3.638IleThr: 3.638 ± 0.047	3.995IleVal: 3.995 ± 0.047	0.834IleTrp: 0.834 ± 0.021	1.691IleTyr: 1.691 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.609LysAla: 3.609 ± 0.048	0.225LysCys: 0.225 ± 0.012	1.764LysAsp: 1.764 ± 0.031	1.962LysGlu: 1.962 ± 0.038	1.188LysPhe: 1.188 ± 0.027	2.12LysGly: 2.12 ± 0.035	0.787LysHis: 0.787 ± 0.019	2.136LysIle: 2.136 ± 0.03	1.643LysLys: 1.643 ± 0.037	4.088LysLeu: 4.088 ± 0.049	0.683LysMet: 0.683 ± 0.018	1.334LysAsn: 1.334 ± 0.027	1.977LysPro: 1.977 ± 0.032	2.263LysGln: 2.263 ± 0.037	2.369LysArg: 2.369 ± 0.033	2.316LysSer: 2.316 ± 0.034	2.492LysThr: 2.492 ± 0.035	2.418LysVal: 2.418 ± 0.042	0.396LysTrp: 0.396 ± 0.014	0.923LysTyr: 0.923 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	10.631LeuAla: 10.631 ± 0.082	1.03LeuCys: 1.03 ± 0.022	6.103LeuAsp: 6.103 ± 0.058	7.059LeuGlu: 7.059 ± 0.069	3.751LeuPhe: 3.751 ± 0.047	8.042LeuGly: 8.042 ± 0.072	1.996LeuHis: 1.996 ± 0.027	6.273LeuIle: 6.273 ± 0.059	4.877LeuLys: 4.877 ± 0.056	11.661LeuLeu: 11.661 ± 0.103	2.415LeuMet: 2.415 ± 0.035	4.354LeuAsn: 4.354 ± 0.049	5.761LeuPro: 5.761 ± 0.064	5.518LeuGln: 5.518 ± 0.064	5.948LeuArg: 5.948 ± 0.056	8.038LeuSer: 8.038 ± 0.07	7.055LeuThr: 7.055 ± 0.058	7.407LeuVal: 7.407 ± 0.071	1.681LeuTrp: 1.681 ± 0.033	2.77LeuTyr: 2.77 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.398MetAla: 2.398 ± 0.036	0.136MetCys: 0.136 ± 0.007	0.926MetAsp: 0.926 ± 0.019	0.962MetGlu: 0.962 ± 0.022	0.606MetPhe: 0.606 ± 0.018	1.613MetGly: 1.613 ± 0.028	0.353MetHis: 0.353 ± 0.014	1.052MetIle: 1.052 ± 0.022	0.76MetLys: 0.76 ± 0.02	2.066MetLeu: 2.066 ± 0.03	0.511MetMet: 0.511 ± 0.017	0.663MetAsn: 0.663 ± 0.019	1.175MetPro: 1.175 ± 0.021	0.935MetGln: 0.935 ± 0.02	1.054MetArg: 1.054 ± 0.02	1.307MetSer: 1.307 ± 0.024	1.411MetThr: 1.411 ± 0.027	1.626MetVal: 1.626 ± 0.027	0.175MetTrp: 0.175 ± 0.009	0.347MetTyr: 0.347 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.002AsnAla: 3.002 ± 0.041	0.368AsnCys: 0.368 ± 0.012	1.86AsnAsp: 1.86 ± 0.045	1.726AsnGlu: 1.726 ± 0.03	1.483AsnPhe: 1.483 ± 0.028	2.583AsnGly: 2.583 ± 0.041	0.876AsnHis: 0.876 ± 0.021	2.193AsnIle: 2.193 ± 0.034	1.035AsnLys: 1.035 ± 0.023	4.552AsnLeu: 4.552 ± 0.061	0.564AsnMet: 0.564 ± 0.015	1.56AsnAsn: 1.56 ± 0.033	2.425AsnPro: 2.425 ± 0.036	2.351AsnGln: 2.351 ± 0.031	2.36AsnArg: 2.36 ± 0.032	2.312AsnSer: 2.312 ± 0.039	2.049AsnThr: 2.049 ± 0.031	2.178AsnVal: 2.178 ± 0.03	0.619AsnTrp: 0.619 ± 0.016	1.195AsnTyr: 1.195 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.969ProAla: 3.969 ± 0.047	0.368ProCys: 0.368 ± 0.017	3.41ProAsp: 3.41 ± 0.046	3.741ProGlu: 3.741 ± 0.053	1.781ProPhe: 1.781 ± 0.027	3.246ProGly: 3.246 ± 0.038	0.992ProHis: 0.992 ± 0.022	2.958ProIle: 2.958 ± 0.041	1.876ProLys: 1.876 ± 0.034	4.977ProLeu: 4.977 ± 0.052	1.03ProMet: 1.03 ± 0.021	1.998ProAsn: 1.998 ± 0.032	2.325ProPro: 2.325 ± 0.04	2.419ProGln: 2.419 ± 0.042	1.907ProArg: 1.907 ± 0.03	3.063ProSer: 3.063 ± 0.037	3.099ProThr: 3.099 ± 0.046	3.414ProVal: 3.414 ± 0.044	0.715ProTrp: 0.715 ± 0.018	1.348ProTyr: 1.348 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	5.463GlnAla: 5.463 ± 0.066	0.413GlnCys: 0.413 ± 0.014	2.348GlnAsp: 2.348 ± 0.03	2.856GlnGlu: 2.856 ± 0.046	1.855GlnPhe: 1.855 ± 0.027	3.584GlnGly: 3.584 ± 0.043	1.073GlnHis: 1.073 ± 0.024	3.122GlnIle: 3.122 ± 0.042	1.921GlnLys: 1.921 ± 0.034	6.648GlnLeu: 6.648 ± 0.074	0.99GlnMet: 0.99 ± 0.021	1.638GlnAsn: 1.638 ± 0.026	2.946GlnPro: 2.946 ± 0.043	4.061GlnGln: 4.061 ± 0.072	3.4GlnArg: 3.4 ± 0.046	3.252GlnSer: 3.252 ± 0.042	3.523GlnThr: 3.523 ± 0.048	3.96GlnVal: 3.96 ± 0.048	0.848GlnTrp: 0.848 ± 0.022	1.281GlnTyr: 1.281 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	3.993ArgAla: 3.993 ± 0.05	0.539ArgCys: 0.539 ± 0.016	2.792ArgAsp: 2.792 ± 0.04	2.916ArgGlu: 2.916 ± 0.04	2.378ArgPhe: 2.378 ± 0.035	3.048ArgGly: 3.048 ± 0.038	1.243ArgHis: 1.243 ± 0.024	3.267ArgIle: 3.267 ± 0.039	1.94ArgLys: 1.94 ± 0.037	6.688ArgLeu: 6.688 ± 0.067	1.124ArgMet: 1.124 ± 0.02	1.87ArgAsn: 1.87 ± 0.03	2.485ArgPro: 2.485 ± 0.04	3.659ArgGln: 3.659 ± 0.052	3.415ArgArg: 3.415 ± 0.05	3.471ArgSer: 3.471 ± 0.036	2.796ArgThr: 2.796 ± 0.042	3.504ArgVal: 3.504 ± 0.039	1.04ArgTrp: 1.04 ± 0.025	2.043ArgTyr: 2.043 ± 0.033	0.0ArgXaa: 0.0 ± 0.001
Ser	5.142SerAla: 5.142 ± 0.048	0.629SerCys: 0.629 ± 0.018	3.511SerAsp: 3.511 ± 0.048	3.597SerGlu: 3.597 ± 0.049	2.591SerPhe: 2.591 ± 0.033	4.784SerGly: 4.784 ± 0.049	1.417SerHis: 1.417 ± 0.027	3.62SerIle: 3.62 ± 0.039	2.16SerLys: 2.16 ± 0.037	7.537SerLeu: 7.537 ± 0.069	1.307SerMet: 1.307 ± 0.026	2.369SerAsn: 2.369 ± 0.043	3.416SerPro: 3.416 ± 0.046	3.552SerGln: 3.552 ± 0.041	3.245SerArg: 3.245 ± 0.04	4.368SerSer: 4.368 ± 0.054	3.603SerThr: 3.603 ± 0.043	4.164SerVal: 4.164 ± 0.043	0.946SerTrp: 0.946 ± 0.023	1.784SerTyr: 1.784 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	5.659ThrAla: 5.659 ± 0.053	0.525ThrCys: 0.525 ± 0.016	3.236ThrAsp: 3.236 ± 0.041	3.177ThrGlu: 3.177 ± 0.043	2.168ThrPhe: 2.168 ± 0.031	4.387ThrGly: 4.387 ± 0.054	1.192ThrHis: 1.192 ± 0.025	3.615ThrIle: 3.615 ± 0.043	1.803ThrLys: 1.803 ± 0.031	7.36ThrLeu: 7.36 ± 0.067	1.041ThrMet: 1.041 ± 0.021	1.994ThrAsn: 1.994 ± 0.035	3.438ThrPro: 3.438 ± 0.041	3.011ThrGln: 3.011 ± 0.044	2.559ThrArg: 2.559 ± 0.033	3.493ThrSer: 3.493 ± 0.044	3.714ThrThr: 3.714 ± 0.045	4.664ThrVal: 4.664 ± 0.056	0.848ThrTrp: 0.848 ± 0.019	1.775ThrTyr: 1.775 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	6.432ValAla: 6.432 ± 0.057	0.693ValCys: 0.693 ± 0.018	3.947ValAsp: 3.947 ± 0.04	4.201ValGlu: 4.201 ± 0.049	2.601ValPhe: 2.601 ± 0.033	4.809ValGly: 4.809 ± 0.051	1.193ValHis: 1.193 ± 0.02	4.166ValIle: 4.166 ± 0.048	2.553ValLys: 2.553 ± 0.035	7.218ValLeu: 7.218 ± 0.066	1.523ValMet: 1.523 ± 0.028	2.563ValAsn: 2.563 ± 0.034	3.144ValPro: 3.144 ± 0.04	2.904ValGln: 2.904 ± 0.034	3.368ValArg: 3.368 ± 0.044	4.573ValSer: 4.573 ± 0.046	4.174ValThr: 4.174 ± 0.047	5.139ValVal: 5.139 ± 0.06	0.933ValTrp: 0.933 ± 0.025	1.77ValTyr: 1.77 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.069TrpAla: 1.069 ± 0.022	0.163TrpCys: 0.163 ± 0.008	0.698TrpAsp: 0.698 ± 0.02	0.751TrpGlu: 0.751 ± 0.019	0.619TrpPhe: 0.619 ± 0.018	0.961TrpGly: 0.961 ± 0.026	0.431TrpHis: 0.431 ± 0.015	0.826TrpIle: 0.826 ± 0.019	0.485TrpLys: 0.485 ± 0.016	2.133TrpLeu: 2.133 ± 0.037	0.338TrpMet: 0.338 ± 0.012	0.508TrpAsn: 0.508 ± 0.016	0.559TrpPro: 0.559 ± 0.02	1.338TrpGln: 1.338 ± 0.024	0.91TrpArg: 0.91 ± 0.021	0.943TrpSer: 0.943 ± 0.021	0.701TrpThr: 0.701 ± 0.016	1.074TrpVal: 1.074 ± 0.024	0.273TrpTrp: 0.273 ± 0.011	0.38TrpTyr: 0.38 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.21TyrAla: 2.21 ± 0.031	0.375TyrCys: 0.375 ± 0.012	1.593TyrAsp: 1.593 ± 0.03	1.649TyrGlu: 1.649 ± 0.03	1.249TyrPhe: 1.249 ± 0.023	2.331TyrGly: 2.331 ± 0.031	0.639TyrHis: 0.639 ± 0.017	1.401TyrIle: 1.401 ± 0.028	0.895TyrLys: 0.895 ± 0.024	3.383TyrLeu: 3.383 ± 0.042	0.458TyrMet: 0.458 ± 0.013	0.895TyrAsn: 0.895 ± 0.023	1.469TyrPro: 1.469 ± 0.028	1.784TyrGln: 1.784 ± 0.03	2.208TyrArg: 2.208 ± 0.034	1.81TyrSer: 1.81 ± 0.03	1.463TyrThr: 1.463 ± 0.026	1.749TyrVal: 1.749 ± 0.026	0.54TyrTrp: 0.54 ± 0.018	0.917TyrTyr: 0.917 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7108 proteins (2230696 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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