Chara braunii (Braun s stonewort)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Charophyceae; Charales; Characeae; Chara

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A388KE62|A0A388KE62_CHABU Uncharacterized protein OS=Chara braunii OX=69332 GN=CBR_g2889 PE=4 SV=1
LL1 pKa = 6.03MRR3 pKa = 11.84HH4 pKa = 5.38YY5 pKa = 10.85YY6 pKa = 10.28EE7 pKa = 4.55EE8 pKa = 4.99LEE10 pKa = 4.29EE11 pKa = 4.7GQDD14 pKa = 3.32LCAALEE20 pKa = 3.85DD21 pKa = 4.04RR22 pKa = 11.84YY23 pKa = 11.22YY24 pKa = 11.49DD25 pKa = 3.62NGDD28 pKa = 4.27NITDD32 pKa = 3.78DD33 pKa = 4.19ANNNNSIANDD43 pKa = 4.25AIKK46 pKa = 10.08TNSCDD51 pKa = 3.82NNNLNYY57 pKa = 10.04IADD60 pKa = 3.87NHH62 pKa = 5.56NHH64 pKa = 5.69NNNNNNYY71 pKa = 9.98NNNKK75 pKa = 8.37TNNNNDD81 pKa = 3.98DD82 pKa = 4.77NNDD85 pKa = 3.58CTDD88 pKa = 3.46NDD90 pKa = 4.71DD91 pKa = 5.91DD92 pKa = 5.02NDD94 pKa = 3.99HH95 pKa = 7.39PEE97 pKa = 5.22DD98 pKa = 4.53GDD100 pKa = 3.72NALIEE105 pKa = 5.17AINNNNNNFSDD116 pKa = 4.28GDD118 pKa = 3.87NNNNNNNNDD127 pKa = 3.49NGNNCDD133 pKa = 4.83LRR135 pKa = 11.84EE136 pKa = 4.43DD137 pKa = 3.77DD138 pKa = 3.76TNVRR142 pKa = 11.84IEE144 pKa = 4.22VIQVVNTNDD153 pKa = 3.84DD154 pKa = 3.99DD155 pKa = 4.97NISYY159 pKa = 10.8EE160 pKa = 4.46DD161 pKa = 3.74NNNTNSDD168 pKa = 3.6GSDD171 pKa = 3.01HH172 pKa = 6.94LFAGDD177 pKa = 3.36MLAFSRR183 pKa = 11.84MHH185 pKa = 6.45LLIGGGRR192 pKa = 11.84PLWEE196 pKa = 5.0GIFLRR201 pKa = 11.84MLMSIWRR208 pKa = 11.84CSYY211 pKa = 10.37GRR213 pKa = 11.84YY214 pKa = 8.09TIASLPWDD222 pKa = 3.95PGGRR226 pKa = 11.84VAGG229 pKa = 4.06

Molecular weight: 25.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A388LMT7|A0A388LMT7_CHABU Uncharacterized protein OS=Chara braunii OX=69332 GN=CBR_g37458 PE=4 SV=1
MM1 pKa = 7.2HH2 pKa = 7.4AAASAAAAAPAAAAAALAAAAAAGPAAAAAAAAGPAAAAGAAAGPAAAAAAAAAAASPRR61 pKa = 11.84PAAAAGAPRR70 pKa = 11.84PAAATRR76 pKa = 11.84HH77 pKa = 5.14PHH79 pKa = 6.2APPAGGARR87 pKa = 11.84AAAPPAAAARR97 pKa = 11.84AAVPLAAAARR107 pKa = 11.84AAAPPAAGPPAAAPRR122 pKa = 11.84AAAPRR127 pKa = 11.84AAVRR131 pKa = 11.84AVAPPAAPRR140 pKa = 11.84AAARR144 pKa = 11.84AAVAPAASAPAARR157 pKa = 11.84AAAAPAAAAPAGRR170 pKa = 11.84AAAAPAAAAASAAPAAAAAAAPTAAAPAATARR202 pKa = 11.84AAASPAAAAPRR213 pKa = 11.84APPPPPHH220 pKa = 6.48HH221 pKa = 6.63HH222 pKa = 6.02HH223 pKa = 6.5HH224 pKa = 5.48VVPRR228 pKa = 11.84VIGPVAGPEE237 pKa = 3.99HH238 pKa = 7.69SVMQSGGG245 pKa = 3.31

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34644

932

35576

23446944

10

8938

659.1

72.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.467 ± 0.013

1.595 ± 0.005

6.145 ± 0.012

8.045 ± 0.025

2.984 ± 0.007

8.263 ± 0.021

2.39 ± 0.007

3.704 ± 0.008

5.13 ± 0.013

8.0 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.005

2.81 ± 0.007

5.305 ± 0.012

4.061 ± 0.01

7.726 ± 0.011

7.209 ± 0.014

5.238 ± 0.009

6.792 ± 0.018

1.477 ± 0.004

2.163 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski