Amino acid dipepetide frequency for Longibaculum muris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.388AlaAla: 3.388 ± 0.087	0.952AlaCys: 0.952 ± 0.034	2.713AlaAsp: 2.713 ± 0.058	2.347AlaGlu: 2.347 ± 0.053	2.799AlaPhe: 2.799 ± 0.057	3.715AlaGly: 3.715 ± 0.093	1.3AlaHis: 1.3 ± 0.043	5.575AlaIle: 5.575 ± 0.089	4.723AlaLys: 4.723 ± 0.075	6.166AlaLeu: 6.166 ± 0.095	2.005AlaMet: 2.005 ± 0.052	2.517AlaAsn: 2.517 ± 0.051	1.409AlaPro: 1.409 ± 0.042	2.008AlaGln: 2.008 ± 0.045	1.997AlaArg: 1.997 ± 0.045	3.592AlaSer: 3.592 ± 0.075	3.048AlaThr: 3.048 ± 0.08	3.557AlaVal: 3.557 ± 0.068	0.438AlaTrp: 0.438 ± 0.021	2.776AlaTyr: 2.776 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.772CysAla: 0.772 ± 0.029	0.247CysCys: 0.247 ± 0.017	0.885CysAsp: 0.885 ± 0.032	0.824CysGlu: 0.824 ± 0.031	0.795CysPhe: 0.795 ± 0.031	1.065CysGly: 1.065 ± 0.037	0.468CysHis: 0.468 ± 0.022	1.25CysIle: 1.25 ± 0.038	0.663CysLys: 0.663 ± 0.025	1.545CysLeu: 1.545 ± 0.045	0.443CysMet: 0.443 ± 0.023	0.467CysAsn: 0.467 ± 0.023	0.525CysPro: 0.525 ± 0.031	0.737CysGln: 0.737 ± 0.029	0.454CysArg: 0.454 ± 0.022	0.828CysSer: 0.828 ± 0.032	0.545CysThr: 0.545 ± 0.023	0.957CysVal: 0.957 ± 0.033	0.081CysTrp: 0.081 ± 0.009	0.708CysTyr: 0.708 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.101AspAla: 3.101 ± 0.059	0.839AspCys: 0.839 ± 0.031	3.718AspAsp: 3.718 ± 0.067	5.597AspGlu: 5.597 ± 0.08	3.105AspPhe: 3.105 ± 0.054	3.639AspGly: 3.639 ± 0.078	1.265AspHis: 1.265 ± 0.039	5.801AspIle: 5.801 ± 0.075	3.987AspLys: 3.987 ± 0.078	5.528AspLeu: 5.528 ± 0.086	1.863AspMet: 1.863 ± 0.046	2.678AspAsn: 2.678 ± 0.063	1.453AspPro: 1.453 ± 0.035	2.125AspGln: 2.125 ± 0.048	1.609AspArg: 1.609 ± 0.045	2.892AspSer: 2.892 ± 0.06	2.472AspThr: 2.472 ± 0.059	4.062AspVal: 4.062 ± 0.065	0.465AspTrp: 0.465 ± 0.024	3.584AspTyr: 3.584 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	3.932GluAla: 3.932 ± 0.072	0.808GluCys: 0.808 ± 0.033	4.161GluAsp: 4.161 ± 0.076	5.454GluGlu: 5.454 ± 0.097	2.71GluPhe: 2.71 ± 0.051	3.566GluGly: 3.566 ± 0.058	1.362GluHis: 1.362 ± 0.04	5.75GluIle: 5.75 ± 0.08	6.455GluLys: 6.455 ± 0.1	6.555GluLeu: 6.555 ± 0.087	2.422GluMet: 2.422 ± 0.046	4.341GluAsn: 4.341 ± 0.078	1.459GluPro: 1.459 ± 0.038	2.299GluGln: 2.299 ± 0.05	2.519GluArg: 2.519 ± 0.054	3.304GluSer: 3.304 ± 0.061	3.257GluThr: 3.257 ± 0.066	4.408GluVal: 4.408 ± 0.076	0.501GluTrp: 0.501 ± 0.025	3.194GluTyr: 3.194 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.525PheAla: 2.525 ± 0.058	0.642PheCys: 0.642 ± 0.027	3.099PheAsp: 3.099 ± 0.054	3.049PheGlu: 3.049 ± 0.057	1.979PhePhe: 1.979 ± 0.054	2.727PheGly: 2.727 ± 0.062	1.127PheHis: 1.127 ± 0.04	4.488PheIle: 4.488 ± 0.086	2.897PheLys: 2.897 ± 0.054	4.165PheLeu: 4.165 ± 0.074	1.331PheMet: 1.331 ± 0.041	2.158PheAsn: 2.158 ± 0.053	1.204PhePro: 1.204 ± 0.034	1.722PheGln: 1.722 ± 0.046	1.1PheArg: 1.1 ± 0.034	2.812PheSer: 2.812 ± 0.06	2.11PheThr: 2.11 ± 0.049	3.2PheVal: 3.2 ± 0.069	0.278PheTrp: 0.278 ± 0.018	2.249PheTyr: 2.249 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	3.635GlyAla: 3.635 ± 0.082	1.051GlyCys: 1.051 ± 0.038	2.904GlyAsp: 2.904 ± 0.064	3.573GlyGlu: 3.573 ± 0.061	3.003GlyPhe: 3.003 ± 0.062	3.835GlyGly: 3.835 ± 0.099	1.382GlyHis: 1.382 ± 0.038	5.696GlyIle: 5.696 ± 0.085	4.669GlyLys: 4.669 ± 0.072	5.527GlyLeu: 5.527 ± 0.095	1.968GlyMet: 1.968 ± 0.053	2.623GlyAsn: 2.623 ± 0.059	1.131GlyPro: 1.131 ± 0.042	2.068GlyGln: 2.068 ± 0.051	1.911GlyArg: 1.911 ± 0.047	3.131GlySer: 3.131 ± 0.07	3.187GlyThr: 3.187 ± 0.069	4.147GlyVal: 4.147 ± 0.067	0.554GlyTrp: 0.554 ± 0.03	3.474GlyTyr: 3.474 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.104HisAla: 1.104 ± 0.035	0.438HisCys: 0.438 ± 0.025	1.401HisAsp: 1.401 ± 0.039	1.403HisGlu: 1.403 ± 0.041	1.299HisPhe: 1.299 ± 0.039	1.3HisGly: 1.3 ± 0.042	0.875HisHis: 0.875 ± 0.035	2.2HisIle: 2.2 ± 0.046	1.238HisLys: 1.238 ± 0.038	2.268HisLeu: 2.268 ± 0.053	0.644HisMet: 0.644 ± 0.029	0.93HisAsn: 0.93 ± 0.035	0.947HisPro: 0.947 ± 0.03	1.428HisGln: 1.428 ± 0.043	0.765HisArg: 0.765 ± 0.026	1.192HisSer: 1.192 ± 0.032	1.01HisThr: 1.01 ± 0.036	1.42HisVal: 1.42 ± 0.037	0.153HisTrp: 0.153 ± 0.013	1.366HisTyr: 1.366 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	5.701IleAla: 5.701 ± 0.088	1.502IleCys: 1.502 ± 0.045	6.1IleAsp: 6.1 ± 0.087	6.531IleGlu: 6.531 ± 0.082	3.695IlePhe: 3.695 ± 0.08	5.394IleGly: 5.394 ± 0.093	2.088IleHis: 2.088 ± 0.059	7.382IleIle: 7.382 ± 0.113	5.961IleLys: 5.961 ± 0.095	8.248IleLeu: 8.248 ± 0.116	2.279IleMet: 2.279 ± 0.057	4.119IleAsn: 4.119 ± 0.075	3.124IlePro: 3.124 ± 0.062	3.654IleGln: 3.654 ± 0.066	2.524IleArg: 2.524 ± 0.064	5.485IleSer: 5.485 ± 0.088	4.2IleThr: 4.2 ± 0.071	6.174IleVal: 6.174 ± 0.093	0.52IleTrp: 0.52 ± 0.025	4.001IleTyr: 4.001 ± 0.089	0.0IleXaa: 0.0 ± 0.0
Lys	4.759LysAla: 4.759 ± 0.096	0.715LysCys: 0.715 ± 0.029	5.481LysAsp: 5.481 ± 0.092	7.422LysGlu: 7.422 ± 0.103	1.937LysPhe: 1.937 ± 0.043	4.07LysGly: 4.07 ± 0.067	1.583LysHis: 1.583 ± 0.038	5.965LysIle: 5.965 ± 0.078	7.148LysLys: 7.148 ± 0.096	5.73LysLeu: 5.73 ± 0.081	2.553LysMet: 2.553 ± 0.054	4.633LysAsn: 4.633 ± 0.078	2.035LysPro: 2.035 ± 0.045	3.186LysGln: 3.186 ± 0.055	3.027LysArg: 3.027 ± 0.055	3.639LysSer: 3.639 ± 0.062	3.968LysThr: 3.968 ± 0.073	4.89LysVal: 4.89 ± 0.083	0.599LysTrp: 0.599 ± 0.028	3.599LysTyr: 3.599 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	5.595LeuAla: 5.595 ± 0.098	1.465LeuCys: 1.465 ± 0.038	5.591LeuAsp: 5.591 ± 0.083	6.04LeuGlu: 6.04 ± 0.084	4.368LeuPhe: 4.368 ± 0.088	5.603LeuGly: 5.603 ± 0.102	1.696LeuHis: 1.696 ± 0.044	7.612LeuIle: 7.612 ± 0.115	8.404LeuLys: 8.404 ± 0.125	9.361LeuLeu: 9.361 ± 0.121	2.87LeuMet: 2.87 ± 0.058	4.954LeuAsn: 4.954 ± 0.072	3.11LeuPro: 3.11 ± 0.053	3.155LeuGln: 3.155 ± 0.063	2.95LeuArg: 2.95 ± 0.057	7.074LeuSer: 7.074 ± 0.108	5.148LeuThr: 5.148 ± 0.093	5.388LeuVal: 5.388 ± 0.078	0.672LeuTrp: 0.672 ± 0.028	4.113LeuTyr: 4.113 ± 0.083	0.0LeuXaa: 0.0 ± 0.0
Met	1.792MetAla: 1.792 ± 0.046	0.386MetCys: 0.386 ± 0.02	1.679MetAsp: 1.679 ± 0.044	1.777MetGlu: 1.777 ± 0.047	1.144MetPhe: 1.144 ± 0.036	1.711MetGly: 1.711 ± 0.043	0.472MetHis: 0.472 ± 0.022	3.074MetIle: 3.074 ± 0.066	3.174MetLys: 3.174 ± 0.062	2.618MetLeu: 2.618 ± 0.053	1.149MetMet: 1.149 ± 0.038	2.187MetAsn: 2.187 ± 0.054	0.903MetPro: 0.903 ± 0.034	1.004MetGln: 1.004 ± 0.031	1.052MetArg: 1.052 ± 0.034	2.037MetSer: 2.037 ± 0.049	1.641MetThr: 1.641 ± 0.038	1.631MetVal: 1.631 ± 0.041	0.172MetTrp: 0.172 ± 0.013	1.051MetTyr: 1.051 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.848AsnAla: 2.848 ± 0.058	0.621AsnCys: 0.621 ± 0.028	3.236AsnAsp: 3.236 ± 0.06	3.84AsnGlu: 3.84 ± 0.068	1.826AsnPhe: 1.826 ± 0.042	3.189AsnGly: 3.189 ± 0.07	1.553AsnHis: 1.553 ± 0.043	4.461AsnIle: 4.461 ± 0.078	3.675AsnLys: 3.675 ± 0.066	3.823AsnLeu: 3.823 ± 0.074	1.374AsnMet: 1.374 ± 0.037	2.495AsnAsn: 2.495 ± 0.061	1.893AsnPro: 1.893 ± 0.043	2.973AsnGln: 2.973 ± 0.064	1.68AsnArg: 1.68 ± 0.041	2.343AsnSer: 2.343 ± 0.057	2.321AsnThr: 2.321 ± 0.057	3.058AsnVal: 3.058 ± 0.054	0.37AsnTrp: 0.37 ± 0.026	2.296AsnTyr: 2.296 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	1.494ProAla: 1.494 ± 0.036	0.395ProCys: 0.395 ± 0.023	1.656ProAsp: 1.656 ± 0.039	1.894ProGlu: 1.894 ± 0.048	1.506ProPhe: 1.506 ± 0.041	1.492ProGly: 1.492 ± 0.035	0.781ProHis: 0.781 ± 0.033	2.667ProIle: 2.667 ± 0.059	1.787ProLys: 1.787 ± 0.044	2.903ProLeu: 2.903 ± 0.063	0.846ProMet: 0.846 ± 0.026	1.237ProAsn: 1.237 ± 0.038	0.522ProPro: 0.522 ± 0.024	1.278ProGln: 1.278 ± 0.034	0.886ProArg: 0.886 ± 0.032	1.751ProSer: 1.751 ± 0.044	1.805ProThr: 1.805 ± 0.052	1.977ProVal: 1.977 ± 0.053	0.246ProTrp: 0.246 ± 0.017	1.569ProTyr: 1.569 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.389GlnAla: 2.389 ± 0.057	0.477GlnCys: 0.477 ± 0.025	2.324GlnAsp: 2.324 ± 0.05	3.001GlnGlu: 3.001 ± 0.069	1.646GlnPhe: 1.646 ± 0.044	2.118GlnGly: 2.118 ± 0.054	0.707GlnHis: 0.707 ± 0.027	3.326GlnIle: 3.326 ± 0.068	3.74GlnLys: 3.74 ± 0.063	4.029GlnLeu: 4.029 ± 0.067	1.358GlnMet: 1.358 ± 0.036	2.17GlnAsn: 2.17 ± 0.052	1.029GlnPro: 1.029 ± 0.03	1.865GlnGln: 1.865 ± 0.052	1.541GlnArg: 1.541 ± 0.045	2.252GlnSer: 2.252 ± 0.058	2.06GlnThr: 2.06 ± 0.048	2.432GlnVal: 2.432 ± 0.054	0.33GlnTrp: 0.33 ± 0.02	2.176GlnTyr: 2.176 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	1.61ArgAla: 1.61 ± 0.045	0.461ArgCys: 0.461 ± 0.021	1.788ArgAsp: 1.788 ± 0.045	2.313ArgGlu: 2.313 ± 0.057	1.6ArgPhe: 1.6 ± 0.037	1.743ArgGly: 1.743 ± 0.044	0.731ArgHis: 0.731 ± 0.025	2.908ArgIle: 2.908 ± 0.058	2.701ArgLys: 2.701 ± 0.058	3.147ArgLeu: 3.147 ± 0.059	1.088ArgMet: 1.088 ± 0.029	1.666ArgAsn: 1.666 ± 0.045	0.854ArgPro: 0.854 ± 0.031	1.423ArgGln: 1.423 ± 0.04	1.271ArgArg: 1.271 ± 0.039	1.555ArgSer: 1.555 ± 0.038	1.414ArgThr: 1.414 ± 0.037	2.125ArgVal: 2.125 ± 0.049	0.266ArgTrp: 0.266 ± 0.018	1.868ArgTyr: 1.868 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	2.992SerAla: 2.992 ± 0.066	0.74SerCys: 0.74 ± 0.028	3.036SerAsp: 3.036 ± 0.07	3.274SerGlu: 3.274 ± 0.056	3.15SerPhe: 3.15 ± 0.069	3.741SerGly: 3.741 ± 0.074	1.65SerHis: 1.65 ± 0.049	5.229SerIle: 5.229 ± 0.087	3.831SerLys: 3.831 ± 0.069	6.477SerLeu: 6.477 ± 0.092	1.709SerMet: 1.709 ± 0.038	2.386SerAsn: 2.386 ± 0.062	1.579SerPro: 1.579 ± 0.039	2.856SerGln: 2.856 ± 0.06	1.977SerArg: 1.977 ± 0.042	3.876SerSer: 3.876 ± 0.079	2.854SerThr: 2.854 ± 0.065	3.663SerVal: 3.663 ± 0.073	0.476SerTrp: 0.476 ± 0.027	2.832SerTyr: 2.832 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	2.672ThrAla: 2.672 ± 0.067	0.657ThrCys: 0.657 ± 0.027	2.423ThrAsp: 2.423 ± 0.054	2.119ThrGlu: 2.119 ± 0.054	2.432ThrPhe: 2.432 ± 0.054	3.257ThrGly: 3.257 ± 0.089	1.289ThrHis: 1.289 ± 0.036	4.901ThrIle: 4.901 ± 0.075	3.555ThrLys: 3.555 ± 0.071	5.339ThrLeu: 5.339 ± 0.073	1.402ThrMet: 1.402 ± 0.038	2.412ThrAsn: 2.412 ± 0.068	1.875ThrPro: 1.875 ± 0.046	1.766ThrGln: 1.766 ± 0.047	1.515ThrArg: 1.515 ± 0.037	3.221ThrSer: 3.221 ± 0.066	2.812ThrThr: 2.812 ± 0.071	3.27ThrVal: 3.27 ± 0.073	0.386ThrTrp: 0.386 ± 0.022	2.586ThrTyr: 2.586 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	3.729ValAla: 3.729 ± 0.065	1.015ValCys: 1.015 ± 0.027	4.021ValAsp: 4.021 ± 0.077	4.423ValGlu: 4.423 ± 0.073	3.022ValPhe: 3.022 ± 0.064	3.995ValGly: 3.995 ± 0.077	1.27ValHis: 1.27 ± 0.035	5.898ValIle: 5.898 ± 0.084	4.772ValLys: 4.772 ± 0.085	6.134ValLeu: 6.134 ± 0.1	1.842ValMet: 1.842 ± 0.039	3.251ValAsn: 3.251 ± 0.059	1.841ValPro: 1.841 ± 0.048	1.884ValGln: 1.884 ± 0.045	1.829ValArg: 1.829 ± 0.044	4.255ValSer: 4.255 ± 0.07	3.329ValThr: 3.329 ± 0.063	4.596ValVal: 4.596 ± 0.079	0.414ValTrp: 0.414 ± 0.021	2.82ValTyr: 2.82 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.384TrpAla: 0.384 ± 0.02	0.125TrpCys: 0.125 ± 0.013	0.412TrpAsp: 0.412 ± 0.024	0.406TrpGlu: 0.406 ± 0.025	0.355TrpPhe: 0.355 ± 0.019	0.511TrpGly: 0.511 ± 0.026	0.194TrpHis: 0.194 ± 0.015	0.663TrpIle: 0.663 ± 0.028	0.523TrpLys: 0.523 ± 0.027	0.796TrpLeu: 0.796 ± 0.027	0.261TrpMet: 0.261 ± 0.016	0.407TrpAsn: 0.407 ± 0.022	0.145TrpPro: 0.145 ± 0.012	0.346TrpGln: 0.346 ± 0.02	0.228TrpArg: 0.228 ± 0.014	0.405TrpSer: 0.405 ± 0.021	0.357TrpThr: 0.357 ± 0.021	0.396TrpVal: 0.396 ± 0.023	0.08TrpTrp: 0.08 ± 0.009	0.368TrpTyr: 0.368 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.604TyrAla: 2.604 ± 0.051	0.721TyrCys: 0.721 ± 0.03	3.156TyrAsp: 3.156 ± 0.06	3.171TyrGlu: 3.171 ± 0.064	2.491TyrPhe: 2.491 ± 0.054	2.797TyrGly: 2.797 ± 0.053	1.591TyrHis: 1.591 ± 0.045	3.835TyrIle: 3.835 ± 0.065	3.033TyrLys: 3.033 ± 0.061	4.81TyrLeu: 4.81 ± 0.077	1.247TyrMet: 1.247 ± 0.036	2.244TyrAsn: 2.244 ± 0.057	1.654TyrPro: 1.654 ± 0.051	3.192TyrGln: 3.192 ± 0.068	1.699TyrArg: 1.699 ± 0.043	2.682TyrSer: 2.682 ± 0.055	2.353TyrThr: 2.353 ± 0.046	2.992TyrVal: 2.992 ± 0.054	0.359TyrTrp: 0.359 ± 0.02	2.712TyrTyr: 2.712 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3164 proteins (953661 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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