Vibrio phage phiV141

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Tawavirus; unclassified Tawavirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7IB88|A0A7D7IB88_9CAUD Uncharacterized protein OS=Vibrio phage phiV141 OX=2723905 GN=phiV141_31 PE=4 SV=1
MM1 pKa = 7.17DD2 pKa = 3.94TKK4 pKa = 10.96VEE6 pKa = 4.05QEE8 pKa = 4.2VFGLDD13 pKa = 3.3MSNPDD18 pKa = 4.2GLDD21 pKa = 3.45DD22 pKa = 5.33LLDD25 pKa = 4.89HH26 pKa = 6.7IHH28 pKa = 7.18DD29 pKa = 4.34EE30 pKa = 4.45EE31 pKa = 5.12VEE33 pKa = 4.25LTNEE37 pKa = 4.16DD38 pKa = 3.25

Molecular weight: 4.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7ERH6|A0A7D7ERH6_9CAUD Uncharacterized protein OS=Vibrio phage phiV141 OX=2723905 GN=phiV141_43 PE=4 SV=1
MM1 pKa = 7.81WLTNGWGCAICGKK14 pKa = 9.53PFTAADD20 pKa = 3.94YY21 pKa = 11.19AVVDD25 pKa = 4.19HH26 pKa = 7.25DD27 pKa = 4.76HH28 pKa = 6.9DD29 pKa = 3.91TGVIRR34 pKa = 11.84GCLHH38 pKa = 5.01NTCNRR43 pKa = 11.84AEE45 pKa = 4.14GEE47 pKa = 4.12MTSFAKK53 pKa = 10.18RR54 pKa = 11.84VTKK57 pKa = 10.71GGDD60 pKa = 3.15YY61 pKa = 10.43RR62 pKa = 11.84QWLINLGIHH71 pKa = 5.33VSKK74 pKa = 11.04GGTASRR80 pKa = 11.84AVHH83 pKa = 6.83RR84 pKa = 11.84IAKK87 pKa = 7.23FTHH90 pKa = 6.24KK91 pKa = 10.23GVSVSDD97 pKa = 3.77YY98 pKa = 11.32LIGLCNYY105 pKa = 9.3LYY107 pKa = 11.05KK108 pKa = 10.58HH109 pKa = 6.23ATPQCRR115 pKa = 11.84MIHH118 pKa = 6.02PSHH121 pKa = 7.04RR122 pKa = 11.84FPNEE126 pKa = 3.21GGNVKK131 pKa = 9.99KK132 pKa = 10.82ANPRR136 pKa = 11.84FRR138 pKa = 11.84KK139 pKa = 9.48FRR141 pKa = 11.84RR142 pKa = 11.84KK143 pKa = 9.27RR144 pKa = 3.3

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13646

38

1412

284.3

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.036 ± 0.739

0.975 ± 0.148

6.119 ± 0.285

6.801 ± 0.247

3.474 ± 0.265

6.786 ± 0.297

2.308 ± 0.165

4.492 ± 0.192

5.855 ± 0.288

7.497 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.246 ± 0.237

4.456 ± 0.199

4.206 ± 0.33

5.386 ± 0.381

4.925 ± 0.244

5.225 ± 0.216

6.024 ± 0.245

7.826 ± 0.298

1.722 ± 0.145

3.642 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski