Amino acid dipepetide frequency for Vibrio phage BONAISHI

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.14AlaAla: 6.14 ± 0.349
0.777AlaCys: 0.777 ± 0.113
4.774AlaAsp: 4.774 ± 0.24
4.652AlaGlu: 4.652 ± 0.251
2.731AlaPhe: 2.731 ± 0.209
4.541AlaGly: 4.541 ± 0.229
1.255AlaHis: 1.255 ± 0.138
5.385AlaIle: 5.385 ± 0.237
4.519AlaLys: 4.519 ± 0.261
6.351AlaLeu: 6.351 ± 0.267
2.365AlaMet: 2.365 ± 0.175
3.952AlaAsn: 3.952 ± 0.258
2.276AlaPro: 2.276 ± 0.17
2.187AlaGln: 2.187 ± 0.193
3.02AlaArg: 3.02 ± 0.201
4.63AlaSer: 4.63 ± 0.272
4.408AlaThr: 4.408 ± 0.285
4.896AlaVal: 4.896 ± 0.249
0.922AlaTrp: 0.922 ± 0.107
2.709AlaTyr: 2.709 ± 0.181
0.0AlaXaa: 0.0 ± 0.0
Cys
0.611CysAla: 0.611 ± 0.077
0.122CysCys: 0.122 ± 0.041
0.588CysAsp: 0.588 ± 0.089
0.511CysGlu: 0.511 ± 0.074
0.511CysPhe: 0.511 ± 0.067
0.633CysGly: 0.633 ± 0.108
0.322CysHis: 0.322 ± 0.055
0.633CysIle: 0.633 ± 0.075
0.588CysLys: 0.588 ± 0.088
0.777CysLeu: 0.777 ± 0.102
0.211CysMet: 0.211 ± 0.05
0.533CysAsn: 0.533 ± 0.076
0.389CysPro: 0.389 ± 0.07
0.278CysGln: 0.278 ± 0.054
0.577CysArg: 0.577 ± 0.089
0.522CysSer: 0.522 ± 0.082
0.566CysThr: 0.566 ± 0.081
0.422CysVal: 0.422 ± 0.072
0.133CysTrp: 0.133 ± 0.041
0.455CysTyr: 0.455 ± 0.067
0.0CysXaa: 0.0 ± 0.0
Asp
4.707AspAla: 4.707 ± 0.238
0.577AspCys: 0.577 ± 0.074
4.474AspAsp: 4.474 ± 0.328
4.807AspGlu: 4.807 ± 0.186
3.42AspPhe: 3.42 ± 0.223
4.574AspGly: 4.574 ± 0.2
1.532AspHis: 1.532 ± 0.126
5.152AspIle: 5.152 ± 0.262
3.964AspLys: 3.964 ± 0.249
6.761AspLeu: 6.761 ± 0.259
1.921AspMet: 1.921 ± 0.139
3.564AspAsn: 3.564 ± 0.185
3.597AspPro: 3.597 ± 0.232
2.043AspGln: 2.043 ± 0.138
2.898AspArg: 2.898 ± 0.206
4.119AspSer: 4.119 ± 0.236
3.742AspThr: 3.742 ± 0.227
4.508AspVal: 4.508 ± 0.236
0.933AspTrp: 0.933 ± 0.095
2.964AspTyr: 2.964 ± 0.187
0.0AspXaa: 0.0 ± 0.0
Glu
4.741GluAla: 4.741 ± 0.236
0.6GluCys: 0.6 ± 0.079
4.497GluAsp: 4.497 ± 0.203
5.007GluGlu: 5.007 ± 0.273
2.731GluPhe: 2.731 ± 0.186
3.841GluGly: 3.841 ± 0.215
1.399GluHis: 1.399 ± 0.134
4.707GluIle: 4.707 ± 0.251
3.764GluLys: 3.764 ± 0.248
6.384GluLeu: 6.384 ± 0.231
2.01GluMet: 2.01 ± 0.184
3.32GluAsn: 3.32 ± 0.164
1.987GluPro: 1.987 ± 0.175
2.165GluGln: 2.165 ± 0.169
3.186GluArg: 3.186 ± 0.179
4.052GluSer: 4.052 ± 0.222
3.975GluThr: 3.975 ± 0.217
4.152GluVal: 4.152 ± 0.218
0.966GluTrp: 0.966 ± 0.118
2.831GluTyr: 2.831 ± 0.178
0.0GluXaa: 0.0 ± 0.0
Phe
2.431PheAla: 2.431 ± 0.16
0.644PheCys: 0.644 ± 0.097
3.075PheAsp: 3.075 ± 0.197
2.143PheGlu: 2.143 ± 0.153
1.832PhePhe: 1.832 ± 0.164
2.332PheGly: 2.332 ± 0.18
0.899PheHis: 0.899 ± 0.096
3.109PheIle: 3.109 ± 0.19
2.765PheLys: 2.765 ± 0.183
3.009PheLeu: 3.009 ± 0.212
1.255PheMet: 1.255 ± 0.1
2.443PheAsn: 2.443 ± 0.167
1.31PhePro: 1.31 ± 0.11
1.132PheGln: 1.132 ± 0.111
1.865PheArg: 1.865 ± 0.145
2.887PheSer: 2.887 ± 0.167
2.831PheThr: 2.831 ± 0.19
2.687PheVal: 2.687 ± 0.163
0.355PheTrp: 0.355 ± 0.063
1.732PheTyr: 1.732 ± 0.143
0.0PheXaa: 0.0 ± 0.0
Gly
3.519GlyAla: 3.519 ± 0.244
0.6GlyCys: 0.6 ± 0.078
4.308GlyAsp: 4.308 ± 0.222
3.631GlyGlu: 3.631 ± 0.229
2.853GlyPhe: 2.853 ± 0.165
3.242GlyGly: 3.242 ± 0.247
1.41GlyHis: 1.41 ± 0.132
4.574GlyIle: 4.574 ± 0.229
3.975GlyLys: 3.975 ± 0.202
4.952GlyLeu: 4.952 ± 0.238
1.843GlyMet: 1.843 ± 0.146
3.497GlyAsn: 3.497 ± 0.233
2.109GlyPro: 2.109 ± 0.15
2.209GlyGln: 2.209 ± 0.163
2.62GlyArg: 2.62 ± 0.19
4.163GlySer: 4.163 ± 0.298
4.363GlyThr: 4.363 ± 0.25
4.075GlyVal: 4.075 ± 0.221
0.822GlyTrp: 0.822 ± 0.097
2.565GlyTyr: 2.565 ± 0.176
0.0GlyXaa: 0.0 ± 0.0
His
1.255HisAla: 1.255 ± 0.122
0.255HisCys: 0.255 ± 0.051
1.355HisAsp: 1.355 ± 0.112
1.31HisGlu: 1.31 ± 0.127
0.922HisPhe: 0.922 ± 0.102
1.477HisGly: 1.477 ± 0.136
0.677HisHis: 0.677 ± 0.104
1.577HisIle: 1.577 ± 0.138
1.343HisLys: 1.343 ± 0.135
1.81HisLeu: 1.81 ± 0.149
0.544HisMet: 0.544 ± 0.078
1.021HisAsn: 1.021 ± 0.106
1.088HisPro: 1.088 ± 0.113
0.699HisGln: 0.699 ± 0.1
1.077HisArg: 1.077 ± 0.089
1.466HisSer: 1.466 ± 0.131
1.21HisThr: 1.21 ± 0.113
1.621HisVal: 1.621 ± 0.13
0.311HisTrp: 0.311 ± 0.058
0.933HisTyr: 0.933 ± 0.105
0.0HisXaa: 0.0 ± 0.0
Ile
5.318IleAla: 5.318 ± 0.237
0.511IleCys: 0.511 ± 0.079
5.851IleAsp: 5.851 ± 0.291
4.952IleGlu: 4.952 ± 0.231
2.243IlePhe: 2.243 ± 0.142
3.664IleGly: 3.664 ± 0.233
1.588IleHis: 1.588 ± 0.152
4.43IleIle: 4.43 ± 0.227
4.363IleLys: 4.363 ± 0.232
4.963IleLeu: 4.963 ± 0.265
1.821IleMet: 1.821 ± 0.15
3.997IleAsn: 3.997 ± 0.231
3.175IlePro: 3.175 ± 0.188
1.876IleGln: 1.876 ± 0.143
3.209IleArg: 3.209 ± 0.228
4.341IleSer: 4.341 ± 0.272
4.585IleThr: 4.585 ± 0.2
4.186IleVal: 4.186 ± 0.201
0.566IleTrp: 0.566 ± 0.087
2.565IleTyr: 2.565 ± 0.165
0.0IleXaa: 0.0 ± 0.0
Lys
4.841LysAla: 4.841 ± 0.25
0.355LysCys: 0.355 ± 0.068
4.286LysAsp: 4.286 ± 0.239
5.152LysGlu: 5.152 ± 0.274
2.387LysPhe: 2.387 ± 0.196
3.631LysGly: 3.631 ± 0.196
1.432LysHis: 1.432 ± 0.144
3.986LysIle: 3.986 ± 0.222
3.764LysLys: 3.764 ± 0.275
5.396LysLeu: 5.396 ± 0.289
1.621LysMet: 1.621 ± 0.139
2.831LysAsn: 2.831 ± 0.177
2.454LysPro: 2.454 ± 0.159
2.276LysGln: 2.276 ± 0.184
2.975LysArg: 2.975 ± 0.21
3.275LysSer: 3.275 ± 0.206
3.431LysThr: 3.431 ± 0.186
3.486LysVal: 3.486 ± 0.213
0.644LysTrp: 0.644 ± 0.088
2.287LysTyr: 2.287 ± 0.199
0.0LysXaa: 0.0 ± 0.0
Leu
6.228LeuAla: 6.228 ± 0.276
0.844LeuCys: 0.844 ± 0.109
6.306LeuAsp: 6.306 ± 0.245
6.006LeuGlu: 6.006 ± 0.255
3.031LeuPhe: 3.031 ± 0.175
4.419LeuGly: 4.419 ± 0.211
1.466LeuHis: 1.466 ± 0.138
5.096LeuIle: 5.096 ± 0.236
6.073LeuLys: 6.073 ± 0.328
6.129LeuLeu: 6.129 ± 0.296
2.454LeuMet: 2.454 ± 0.203
4.152LeuAsn: 4.152 ± 0.219
3.697LeuPro: 3.697 ± 0.17
2.542LeuGln: 2.542 ± 0.164
4.03LeuArg: 4.03 ± 0.204
6.351LeuSer: 6.351 ± 0.283
5.662LeuThr: 5.662 ± 0.254
5.263LeuVal: 5.263 ± 0.271
0.899LeuTrp: 0.899 ± 0.103
2.909LeuTyr: 2.909 ± 0.186
0.0LeuXaa: 0.0 ± 0.0
Met
2.154MetAla: 2.154 ± 0.166
0.266MetCys: 0.266 ± 0.062
1.721MetAsp: 1.721 ± 0.148
1.832MetGlu: 1.832 ± 0.16
1.166MetPhe: 1.166 ± 0.117
1.61MetGly: 1.61 ± 0.141
0.677MetHis: 0.677 ± 0.099
1.943MetIle: 1.943 ± 0.157
1.321MetLys: 1.321 ± 0.137
2.387MetLeu: 2.387 ± 0.174
0.866MetMet: 0.866 ± 0.114
1.388MetAsn: 1.388 ± 0.136
1.366MetPro: 1.366 ± 0.137
1.166MetGln: 1.166 ± 0.13
1.654MetArg: 1.654 ± 0.157
2.443MetSer: 2.443 ± 0.147
2.043MetThr: 2.043 ± 0.153
1.554MetVal: 1.554 ± 0.118
0.244MetTrp: 0.244 ± 0.056
0.955MetTyr: 0.955 ± 0.09
0.0MetXaa: 0.0 ± 0.0
Asn
3.853AsnAla: 3.853 ± 0.243
0.444AsnCys: 0.444 ± 0.078
3.42AsnAsp: 3.42 ± 0.179
3.053AsnGlu: 3.053 ± 0.182
2.043AsnPhe: 2.043 ± 0.136
4.03AsnGly: 4.03 ± 0.249
1.177AsnHis: 1.177 ± 0.114
3.464AsnIle: 3.464 ± 0.184
3.086AsnLys: 3.086 ± 0.224
3.886AsnLeu: 3.886 ± 0.234
1.421AsnMet: 1.421 ± 0.137
2.798AsnAsn: 2.798 ± 0.164
2.72AsnPro: 2.72 ± 0.191
2.043AsnGln: 2.043 ± 0.189
2.687AsnArg: 2.687 ± 0.159
3.442AsnSer: 3.442 ± 0.202
2.731AsnThr: 2.731 ± 0.214
3.309AsnVal: 3.309 ± 0.232
0.922AsnTrp: 0.922 ± 0.116
1.865AsnTyr: 1.865 ± 0.14
0.0AsnXaa: 0.0 ± 0.0
Pro
2.931ProAla: 2.931 ± 0.204
0.322ProCys: 0.322 ± 0.063
3.053ProAsp: 3.053 ± 0.196
3.486ProGlu: 3.486 ± 0.224
1.732ProPhe: 1.732 ± 0.138
2.72ProGly: 2.72 ± 0.197
0.733ProHis: 0.733 ± 0.103
2.531ProIle: 2.531 ± 0.172
2.343ProLys: 2.343 ± 0.181
2.975ProLeu: 2.975 ± 0.152
0.899ProMet: 0.899 ± 0.103
2.132ProAsn: 2.132 ± 0.197
1.077ProPro: 1.077 ± 0.127
1.155ProGln: 1.155 ± 0.115
1.799ProArg: 1.799 ± 0.156
2.898ProSer: 2.898 ± 0.179
2.087ProThr: 2.087 ± 0.167
3.242ProVal: 3.242 ± 0.194
0.5ProTrp: 0.5 ± 0.072
1.577ProTyr: 1.577 ± 0.17
0.0ProXaa: 0.0 ± 0.0
Gln
2.853GlnAla: 2.853 ± 0.192
0.3GlnCys: 0.3 ± 0.061
1.91GlnAsp: 1.91 ± 0.146
2.065GlnGlu: 2.065 ± 0.153
1.132GlnPhe: 1.132 ± 0.128
2.01GlnGly: 2.01 ± 0.145
0.722GlnHis: 0.722 ± 0.08
2.076GlnIle: 2.076 ± 0.139
1.676GlnLys: 1.676 ± 0.14
2.975GlnLeu: 2.975 ± 0.208
0.955GlnMet: 0.955 ± 0.106
1.554GlnAsn: 1.554 ± 0.125
1.021GlnPro: 1.021 ± 0.167
1.21GlnGln: 1.21 ± 0.177
1.388GlnArg: 1.388 ± 0.146
1.954GlnSer: 1.954 ± 0.141
2.165GlnThr: 2.165 ± 0.148
2.065GlnVal: 2.065 ± 0.152
0.555GlnTrp: 0.555 ± 0.084
1.488GlnTyr: 1.488 ± 0.124
0.0GlnXaa: 0.0 ± 0.0
Arg
3.342ArgAla: 3.342 ± 0.204
0.477ArgCys: 0.477 ± 0.079
3.342ArgAsp: 3.342 ± 0.195
2.809ArgGlu: 2.809 ± 0.216
1.943ArgPhe: 1.943 ± 0.119
2.798ArgGly: 2.798 ± 0.193
1.099ArgHis: 1.099 ± 0.112
3.342ArgIle: 3.342 ± 0.178
2.876ArgLys: 2.876 ± 0.187
4.23ArgLeu: 4.23 ± 0.216
1.61ArgMet: 1.61 ± 0.139
2.431ArgAsn: 2.431 ± 0.185
1.621ArgPro: 1.621 ± 0.129
1.643ArgGln: 1.643 ± 0.141
2.498ArgArg: 2.498 ± 0.229
2.931ArgSer: 2.931 ± 0.181
2.798ArgThr: 2.798 ± 0.171
2.709ArgVal: 2.709 ± 0.137
0.633ArgTrp: 0.633 ± 0.08
2.132ArgTyr: 2.132 ± 0.17
0.0ArgXaa: 0.0 ± 0.0
Ser
4.585SerAla: 4.585 ± 0.244
0.566SerCys: 0.566 ± 0.079
4.53SerAsp: 4.53 ± 0.262
3.631SerGlu: 3.631 ± 0.224
3.02SerPhe: 3.02 ± 0.218
4.519SerGly: 4.519 ± 0.289
1.532SerHis: 1.532 ± 0.163
4.463SerIle: 4.463 ± 0.209
3.531SerLys: 3.531 ± 0.208
5.607SerLeu: 5.607 ± 0.228
1.821SerMet: 1.821 ± 0.12
3.575SerAsn: 3.575 ± 0.178
2.831SerPro: 2.831 ± 0.172
2.121SerGln: 2.121 ± 0.152
3.064SerArg: 3.064 ± 0.224
4.474SerSer: 4.474 ± 0.255
3.553SerThr: 3.553 ± 0.223
4.73SerVal: 4.73 ± 0.249
0.799SerTrp: 0.799 ± 0.092
2.698SerTyr: 2.698 ± 0.183
0.0SerXaa: 0.0 ± 0.0
Thr
5.14ThrAla: 5.14 ± 0.337
0.5ThrCys: 0.5 ± 0.084
3.997ThrAsp: 3.997 ± 0.214
4.064ThrGlu: 4.064 ± 0.181
2.287ThrPhe: 2.287 ± 0.163
4.707ThrGly: 4.707 ± 0.254
1.31ThrHis: 1.31 ± 0.118
3.908ThrIle: 3.908 ± 0.213
3.264ThrLys: 3.264 ± 0.223
5.218ThrLeu: 5.218 ± 0.248
1.932ThrMet: 1.932 ± 0.138
2.942ThrAsn: 2.942 ± 0.213
2.72ThrPro: 2.72 ± 0.147
1.732ThrGln: 1.732 ± 0.14
2.52ThrArg: 2.52 ± 0.162
3.542ThrSer: 3.542 ± 0.228
3.586ThrThr: 3.586 ± 0.215
4.485ThrVal: 4.485 ± 0.217
0.922ThrTrp: 0.922 ± 0.104
2.387ThrTyr: 2.387 ± 0.163
0.0ThrXaa: 0.0 ± 0.0
Val
4.352ValAla: 4.352 ± 0.174
0.622ValCys: 0.622 ± 0.094
4.741ValAsp: 4.741 ± 0.211
4.019ValGlu: 4.019 ± 0.209
2.798ValPhe: 2.798 ± 0.193
3.131ValGly: 3.131 ± 0.208
1.421ValHis: 1.421 ± 0.112
4.397ValIle: 4.397 ± 0.238
4.497ValLys: 4.497 ± 0.215
5.218ValLeu: 5.218 ± 0.251
1.632ValMet: 1.632 ± 0.168
3.686ValAsn: 3.686 ± 0.236
2.698ValPro: 2.698 ± 0.158
1.943ValGln: 1.943 ± 0.163
3.098ValArg: 3.098 ± 0.168
4.641ValSer: 4.641 ± 0.209
4.585ValThr: 4.585 ± 0.225
4.23ValVal: 4.23 ± 0.191
0.722ValTrp: 0.722 ± 0.082
2.753ValTyr: 2.753 ± 0.176
0.0ValXaa: 0.0 ± 0.0
Trp
0.755TrpAla: 0.755 ± 0.095
0.122TrpCys: 0.122 ± 0.043
0.977TrpAsp: 0.977 ± 0.118
0.766TrpGlu: 0.766 ± 0.11
0.5TrpPhe: 0.5 ± 0.067
0.611TrpGly: 0.611 ± 0.079
0.322TrpHis: 0.322 ± 0.064
0.822TrpIle: 0.822 ± 0.094
0.844TrpLys: 0.844 ± 0.108
1.155TrpLeu: 1.155 ± 0.123
0.4TrpMet: 0.4 ± 0.064
0.722TrpAsn: 0.722 ± 0.081
0.444TrpPro: 0.444 ± 0.068
0.311TrpGln: 0.311 ± 0.063
0.722TrpArg: 0.722 ± 0.102
1.01TrpSer: 1.01 ± 0.111
0.655TrpThr: 0.655 ± 0.088
0.733TrpVal: 0.733 ± 0.095
0.155TrpTrp: 0.155 ± 0.046
0.566TrpTyr: 0.566 ± 0.076
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.731TyrAla: 2.731 ± 0.176
0.477TyrCys: 0.477 ± 0.095
3.053TyrAsp: 3.053 ± 0.225
2.431TyrGlu: 2.431 ± 0.156
1.454TyrPhe: 1.454 ± 0.126
2.842TyrGly: 2.842 ± 0.21
0.977TyrHis: 0.977 ± 0.099
2.609TyrIle: 2.609 ± 0.177
1.954TyrLys: 1.954 ± 0.133
3.375TyrLeu: 3.375 ± 0.189
1.166TyrMet: 1.166 ± 0.111
1.854TyrAsn: 1.854 ± 0.186
1.61TyrPro: 1.61 ± 0.14
1.343TyrGln: 1.343 ± 0.115
2.376TyrArg: 2.376 ± 0.202
2.509TyrSer: 2.509 ± 0.183
2.187TyrThr: 2.187 ± 0.171
2.831TyrVal: 2.831 ± 0.191
0.588TyrTrp: 0.588 ± 0.093
1.643TyrTyr: 1.643 ± 0.137
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 301 proteins (90071 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski