Acinetobacter phage BS46

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C2IE34|A0A5C2IE34_9CAUD DNA polymerase III alpha subunit OS=Acinetobacter phage BS46 OX=2606591 GN=BS46_gp79 PE=4 SV=1
MM1 pKa = 6.54TTNFSILIIDD11 pKa = 4.27GLAIEE16 pKa = 4.35SVYY19 pKa = 10.53VRR21 pKa = 11.84EE22 pKa = 4.29LTSEE26 pKa = 4.17SEE28 pKa = 4.19YY29 pKa = 11.35DD30 pKa = 4.01LSDD33 pKa = 3.07VDD35 pKa = 3.93QVVDD39 pKa = 5.16FVAYY43 pKa = 10.08FLGLEE48 pKa = 3.95NSTLIIEE55 pKa = 4.55KK56 pKa = 9.03YY57 pKa = 10.59SRR59 pKa = 11.84FARR62 pKa = 11.84YY63 pKa = 9.55ASINYY68 pKa = 8.41EE69 pKa = 3.57DD70 pKa = 3.95TYY72 pKa = 11.03GVKK75 pKa = 10.22EE76 pKa = 5.46IILTWEE82 pKa = 3.91NFQQ85 pKa = 4.48

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C2ICU4|A0A5C2ICU4_9CAUD Putative recombination protein OS=Acinetobacter phage BS46 OX=2606591 GN=BS46_gp168 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.39IKK4 pKa = 10.56DD5 pKa = 3.55RR6 pKa = 11.84EE7 pKa = 3.83RR8 pKa = 11.84LKK10 pKa = 10.73RR11 pKa = 11.84IEE13 pKa = 4.03YY14 pKa = 10.35NYY16 pKa = 10.08TEE18 pKa = 4.2YY19 pKa = 11.22GYY21 pKa = 10.42IPLNQWRR28 pKa = 11.84EE29 pKa = 3.94YY30 pKa = 10.69YY31 pKa = 10.08RR32 pKa = 11.84LKK34 pKa = 10.82ALRR37 pKa = 11.84KK38 pKa = 8.88IIKK41 pKa = 9.85LKK43 pKa = 9.32RR44 pKa = 11.84ANRR47 pKa = 11.84ILADD51 pKa = 3.4KK52 pKa = 10.54RR53 pKa = 11.84RR54 pKa = 11.84NLQVEE59 pKa = 5.1VYY61 pKa = 8.71TKK63 pKa = 10.5SLSRR67 pKa = 11.84GWTISITACTHH78 pKa = 5.93EE79 pKa = 4.16IARR82 pKa = 11.84LLEE85 pKa = 4.02QIGRR89 pKa = 11.84NSSKK93 pKa = 10.1IQRR96 pKa = 11.84LRR98 pKa = 11.84NGYY101 pKa = 7.63TKK103 pKa = 10.64FSDD106 pKa = 3.01KK107 pKa = 10.59WVDD110 pKa = 3.32SQVNKK115 pKa = 10.18KK116 pKa = 9.59PRR118 pKa = 11.84YY119 pKa = 7.5TII121 pKa = 3.58

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

176

0

176

28117

40

825

159.8

18.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.129 ± 0.255

1.405 ± 0.098

6.608 ± 0.183

7.44 ± 0.235

4.577 ± 0.14

6.085 ± 0.223

1.736 ± 0.115

6.992 ± 0.144

7.903 ± 0.269

8.436 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.088

5.929 ± 0.171

2.465 ± 0.118

3.333 ± 0.158

3.973 ± 0.135

6.779 ± 0.194

5.744 ± 0.217

6.953 ± 0.161

1.348 ± 0.07

4.801 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski