Blastococcus saxobsidens (strain DD2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; Blastococcus saxobsidens

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6RUH3|H6RUH3_BLASD ESAT-6-like protein OS=Blastococcus saxobsidens (strain DD2) OX=1146883 GN=BLASA_0990 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.3LSKK5 pKa = 10.55RR6 pKa = 11.84NSGLVATAIAAALLMTACGGGDD28 pKa = 3.62DD29 pKa = 4.4EE30 pKa = 5.63GGEE33 pKa = 4.61GGGSADD39 pKa = 3.43GGGSYY44 pKa = 10.84SLAINNPEE52 pKa = 3.88NPLVPGNTTEE62 pKa = 4.37SEE64 pKa = 4.24GAQVIKK70 pKa = 10.71ALWTGLIEE78 pKa = 4.05YY79 pKa = 10.58AEE81 pKa = 4.8DD82 pKa = 4.83GEE84 pKa = 4.44VQYY87 pKa = 11.03TGIAEE92 pKa = 4.42SIEE95 pKa = 4.35SDD97 pKa = 5.33DD98 pKa = 3.61NTTWTITLKK107 pKa = 10.95DD108 pKa = 3.06GWTFHH113 pKa = 7.56DD114 pKa = 4.47GTPVTASSFVDD125 pKa = 3.19AWNYY129 pKa = 7.22TAYY132 pKa = 10.7SPNAQGGSYY141 pKa = 10.2FLSNIVGYY149 pKa = 10.65KK150 pKa = 10.18DD151 pKa = 3.5LQAPTDD157 pKa = 3.79DD158 pKa = 4.21AGEE161 pKa = 4.11ITGDD165 pKa = 3.63PVATEE170 pKa = 3.92MTGLEE175 pKa = 4.29VVDD178 pKa = 4.51DD179 pKa = 3.75QTFTVTLNDD188 pKa = 3.84PFAQFPVTVGYY199 pKa = 10.29NAFFPMAEE207 pKa = 4.37AFFEE211 pKa = 4.57DD212 pKa = 4.58PEE214 pKa = 4.68AAALDD219 pKa = 4.2TPIGTGPFQAEE230 pKa = 4.72GPFEE234 pKa = 4.36EE235 pKa = 5.25GVGITLSKK243 pKa = 10.31YY244 pKa = 10.65DD245 pKa = 4.37DD246 pKa = 3.7YY247 pKa = 12.02AGEE250 pKa = 4.16NEE252 pKa = 4.39ANAEE256 pKa = 4.05TLEE259 pKa = 4.43IDD261 pKa = 3.95VYY263 pKa = 11.28SEE265 pKa = 3.47ITTSYY270 pKa = 11.17RR271 pKa = 11.84DD272 pKa = 3.65VQGGNLDD279 pKa = 3.72VVKK282 pKa = 10.44DD283 pKa = 3.84IPADD287 pKa = 3.87VIATAPAEE295 pKa = 4.21FGDD298 pKa = 3.98RR299 pKa = 11.84FIEE302 pKa = 4.46RR303 pKa = 11.84PSSSFTYY310 pKa = 10.4LGVPTYY316 pKa = 10.77DD317 pKa = 3.36PRR319 pKa = 11.84FADD322 pKa = 3.13KK323 pKa = 10.42RR324 pKa = 11.84VRR326 pKa = 11.84QAFSMAIDD334 pKa = 3.82RR335 pKa = 11.84GAITDD340 pKa = 4.72AIFNGTFTPATDD352 pKa = 5.04AIAPVVDD359 pKa = 5.17GYY361 pKa = 11.49RR362 pKa = 11.84EE363 pKa = 4.25GACEE367 pKa = 3.86YY368 pKa = 11.03CEE370 pKa = 5.23LDD372 pKa = 3.89VEE374 pKa = 5.0RR375 pKa = 11.84ANQLLDD381 pKa = 3.26EE382 pKa = 5.15AGFDD386 pKa = 3.15RR387 pKa = 11.84SMPVDD392 pKa = 3.49LWFNAGASHH401 pKa = 6.94DD402 pKa = 3.73AWVEE406 pKa = 3.74AVGNQLRR413 pKa = 11.84EE414 pKa = 4.05NLGIEE419 pKa = 4.1YY420 pKa = 9.01TLQGNLEE427 pKa = 4.01FAEE430 pKa = 4.24YY431 pKa = 10.78LPLLDD436 pKa = 4.45SKK438 pKa = 11.4GITGPFRR445 pKa = 11.84LGWAMDD451 pKa = 3.73YY452 pKa = 10.19PSPQNYY458 pKa = 9.97LEE460 pKa = 4.44PLYY463 pKa = 10.24STAALPPAGSNVTFYY478 pKa = 11.46SNPEE482 pKa = 3.64FDD484 pKa = 4.25QLIAQGNQAEE494 pKa = 4.57TNEE497 pKa = 4.11EE498 pKa = 4.21AIDD501 pKa = 4.33FYY503 pKa = 11.52QQADD507 pKa = 4.22DD508 pKa = 5.75IILEE512 pKa = 4.37DD513 pKa = 3.78MPIIPMFFGVEE524 pKa = 4.07QIVHH528 pKa = 5.93SEE530 pKa = 4.08DD531 pKa = 3.39VSDD534 pKa = 4.04VVIDD538 pKa = 3.42VFGQIDD544 pKa = 3.65TARR547 pKa = 11.84VKK549 pKa = 11.06VNN551 pKa = 3.1

Molecular weight: 59.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6RPY1|H6RPY1_BLASD Lysine--tRNA ligase OS=Blastococcus saxobsidens (strain DD2) OX=1146883 GN=lysS PE=3 SV=1
MM1 pKa = 7.46GKK3 pKa = 9.97GGRR6 pKa = 11.84MSILGKK12 pKa = 10.69LLGTAGGAARR22 pKa = 11.84GAARR26 pKa = 11.84GGRR29 pKa = 11.84ATGRR33 pKa = 11.84PGRR36 pKa = 11.84GVPRR40 pKa = 11.84SAMGRR45 pKa = 11.84TTGRR49 pKa = 11.84RR50 pKa = 11.84GRR52 pKa = 11.84GTPASSGGIAGMLGSLLRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 4.1

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4791

0

4791

1451551

10

1972

303.0

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.145 ± 0.059

0.718 ± 0.009

6.206 ± 0.03

5.66 ± 0.03

2.569 ± 0.024

9.589 ± 0.03

2.041 ± 0.017

3.006 ± 0.027

1.409 ± 0.021

10.634 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.696 ± 0.015

1.457 ± 0.017

6.242 ± 0.032

2.788 ± 0.019

8.308 ± 0.044

4.913 ± 0.025

5.916 ± 0.026

9.51 ± 0.031

1.452 ± 0.015

1.74 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski