Pyrinomonas methylaliphatogenes

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Blastocatellia; Blastocatellales; Pyrinomonadaceae; Pyrinomonas

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B6X3J1|A0A0B6X3J1_9BACT Predicted hydrolase of the alpha/beta superfamily OS=Pyrinomonas methylaliphatogenes OX=454194 GN=PYK22_02909 PE=4 SV=1
MM1 pKa = 7.37CTPTPYY7 pKa = 10.41NGVPAAVPGKK17 pKa = 10.46VEE19 pKa = 3.9AEE21 pKa = 4.28EE22 pKa = 4.37YY23 pKa = 10.98DD24 pKa = 4.85DD25 pKa = 6.73DD26 pKa = 5.1GDD28 pKa = 4.17CDD30 pKa = 3.78GFYY33 pKa = 10.87VPPYY37 pKa = 10.01YY38 pKa = 10.08EE39 pKa = 5.2PKK41 pKa = 10.12PDD43 pKa = 5.14DD44 pKa = 3.98PDD46 pKa = 3.14IVYY49 pKa = 10.26NYY51 pKa = 10.29PEE53 pKa = 4.43VLPVDD58 pKa = 4.86LNAPDD63 pKa = 4.42KK64 pKa = 9.82EE65 pKa = 4.41EE66 pKa = 3.93KK67 pKa = 10.09VAMVGGEE74 pKa = 4.2WINYY78 pKa = 6.17TISVTVAGEE87 pKa = 3.68YY88 pKa = 10.39RR89 pKa = 11.84FAARR93 pKa = 11.84VASAVDD99 pKa = 3.67GEE101 pKa = 4.57SFHH104 pKa = 8.25VKK106 pKa = 9.31IDD108 pKa = 3.55GVNRR112 pKa = 11.84TGSVIIPNTGSWDD125 pKa = 3.56SYY127 pKa = 11.14QMVEE131 pKa = 3.97ADD133 pKa = 4.41LGYY136 pKa = 10.74LEE138 pKa = 5.21AGRR141 pKa = 11.84HH142 pKa = 4.19VMRR145 pKa = 11.84LAIEE149 pKa = 4.47GNSEE153 pKa = 3.85KK154 pKa = 10.62SGHH157 pKa = 5.96FDD159 pKa = 3.42YY160 pKa = 11.44YY161 pKa = 11.34LLTLPPPCTDD171 pKa = 3.28NDD173 pKa = 3.87GDD175 pKa = 4.84GYY177 pKa = 11.18CSDD180 pKa = 4.49VDD182 pKa = 4.27CDD184 pKa = 4.74DD185 pKa = 4.52WDD187 pKa = 4.0PLNYY191 pKa = 9.87PGAPRR196 pKa = 11.84DD197 pKa = 3.58CSSWVDD203 pKa = 3.63RR204 pKa = 11.84DD205 pKa = 3.52CDD207 pKa = 4.29GIPNRR212 pKa = 11.84EE213 pKa = 3.95EE214 pKa = 4.2CSFGGPGPILSLLDD228 pKa = 3.77SFDD231 pKa = 2.9RR232 pKa = 11.84WFAFNSRR239 pKa = 11.84LGADD243 pKa = 4.7LIRR246 pKa = 11.84PVSMRR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84CRR255 pKa = 3.26

Molecular weight: 28.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B6X150|A0A0B6X150_9BACT Predicted nucleotidyltransferase OS=Pyrinomonas methylaliphatogenes OX=454194 GN=PYK22_02750 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.44RR3 pKa = 11.84IALVLAFVLTLGLGSFAAAQTTSGQNTNTSASSSASTAKK42 pKa = 9.65TKK44 pKa = 9.15RR45 pKa = 11.84HH46 pKa = 4.83KK47 pKa = 10.06RR48 pKa = 11.84AHH50 pKa = 5.62KK51 pKa = 9.74RR52 pKa = 11.84AKK54 pKa = 9.88KK55 pKa = 10.45SKK57 pKa = 9.21ATNTNANTNTSRR69 pKa = 3.76

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3179

0

3179

1112582

29

2744

350.0

38.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.027 ± 0.064

0.884 ± 0.016

5.055 ± 0.028

7.183 ± 0.058

3.94 ± 0.035

7.581 ± 0.042

1.879 ± 0.018

5.871 ± 0.031

3.132 ± 0.033

10.258 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.915 ± 0.017

2.846 ± 0.039

4.827 ± 0.034

3.247 ± 0.028

9.118 ± 0.043

5.261 ± 0.028

4.99 ± 0.03

7.0 ± 0.031

1.246 ± 0.019

2.739 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski