Sclerotinia sclerotiorum negative-stranded RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Mymonaviridae; Sclerotimonavirus; Sclerotimonavirus sclerotiniae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5ETK0|X5ETK0_9MONO Large structural protein OS=Sclerotinia sclerotiorum negative-stranded RNA virus 1 OX=1483724 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 3.7VLEE5 pKa = 4.85NQPQFDD11 pKa = 4.55FYY13 pKa = 11.76SFVCALVLLEE23 pKa = 5.21DD24 pKa = 3.34LTKK27 pKa = 10.55RR28 pKa = 11.84VWEE31 pKa = 4.31SNLIEE36 pKa = 4.26TSSEE40 pKa = 3.8FSLVTEE46 pKa = 4.61DD47 pKa = 4.85GEE49 pKa = 4.37FEE51 pKa = 4.24EE52 pKa = 5.54VEE54 pKa = 4.42VTSGSNLGG62 pKa = 3.56

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5FC24|X5FC24_9MONO Nucleoprotein OS=Sclerotinia sclerotiorum negative-stranded RNA virus 1 OX=1483724 PE=4 SV=1
MM1 pKa = 7.76PSYY4 pKa = 10.45KK5 pKa = 10.02EE6 pKa = 4.22KK7 pKa = 11.05YY8 pKa = 9.86FSICNRR14 pKa = 11.84LTTMEE19 pKa = 4.17RR20 pKa = 11.84RR21 pKa = 11.84VLLDD25 pKa = 3.21EE26 pKa = 6.17SILLDD31 pKa = 4.25LRR33 pKa = 11.84QTILNLQTQLNNPQKK48 pKa = 10.57EE49 pKa = 3.93LDD51 pKa = 3.59EE52 pKa = 4.33FLINQLHH59 pKa = 6.26LALTEE64 pKa = 4.49LIKK67 pKa = 10.86YY68 pKa = 8.67ANPQFQLPAHH78 pKa = 6.49FKK80 pKa = 10.18GKK82 pKa = 9.9EE83 pKa = 3.6INEE86 pKa = 4.01ILTITTEE93 pKa = 4.11MIFNAVSLLRR103 pKa = 11.84DD104 pKa = 3.48ATVRR108 pKa = 11.84AQTIISDD115 pKa = 3.97IPSKK119 pKa = 10.21PIKK122 pKa = 10.26DD123 pKa = 3.79NPPSRR128 pKa = 11.84NHH130 pKa = 6.94LIKK133 pKa = 10.85NRR135 pKa = 11.84TLNNEE140 pKa = 3.41SRR142 pKa = 11.84IILKK146 pKa = 10.67SSTTEE151 pKa = 3.87LSPEE155 pKa = 3.9PSRR158 pKa = 11.84NRR160 pKa = 11.84PRR162 pKa = 11.84SFTMSPP168 pKa = 3.01

Molecular weight: 19.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3050

62

1934

508.3

57.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.574 ± 1.265

1.377 ± 0.26

5.443 ± 0.338

6.033 ± 0.914

4.328 ± 0.698

4.066 ± 0.486

2.131 ± 0.283

7.443 ± 0.451

5.902 ± 0.572

10.885 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.089

4.689 ± 0.617

5.049 ± 0.329

3.639 ± 0.457

5.705 ± 0.424

9.344 ± 0.128

6.721 ± 0.772

4.754 ± 0.588

1.082 ± 0.37

3.639 ± 0.698
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski