Amino acid dipepetide frequency for Flavobacterium lacus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.038AlaAla: 4.038 ± 0.08
0.519AlaCys: 0.519 ± 0.027
3.123AlaAsp: 3.123 ± 0.064
3.953AlaGlu: 3.953 ± 0.077
3.324AlaPhe: 3.324 ± 0.07
4.115AlaGly: 4.115 ± 0.09
0.936AlaHis: 0.936 ± 0.027
5.343AlaIle: 5.343 ± 0.075
4.447AlaLys: 4.447 ± 0.076
5.77AlaLeu: 5.77 ± 0.083
1.381AlaMet: 1.381 ± 0.037
3.707AlaAsn: 3.707 ± 0.077
1.898AlaPro: 1.898 ± 0.057
2.434AlaGln: 2.434 ± 0.045
1.756AlaArg: 1.756 ± 0.042
3.954AlaSer: 3.954 ± 0.08
4.267AlaThr: 4.267 ± 0.114
4.124AlaVal: 4.124 ± 0.072
0.548AlaTrp: 0.548 ± 0.026
2.108AlaTyr: 2.108 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.481CysAla: 0.481 ± 0.026
0.118CysCys: 0.118 ± 0.013
0.468CysAsp: 0.468 ± 0.026
0.528CysGlu: 0.528 ± 0.024
0.458CysPhe: 0.458 ± 0.023
0.701CysGly: 0.701 ± 0.031
0.166CysHis: 0.166 ± 0.013
0.536CysIle: 0.536 ± 0.024
0.451CysLys: 0.451 ± 0.022
0.676CysLeu: 0.676 ± 0.028
0.111CysMet: 0.111 ± 0.01
0.441CysAsn: 0.441 ± 0.029
0.331CysPro: 0.331 ± 0.019
0.292CysGln: 0.292 ± 0.019
0.186CysArg: 0.186 ± 0.015
0.644CysSer: 0.644 ± 0.032
0.469CysThr: 0.469 ± 0.031
0.462CysVal: 0.462 ± 0.023
0.061CysTrp: 0.061 ± 0.008
0.327CysTyr: 0.327 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.318AspAla: 3.318 ± 0.059
0.446AspCys: 0.446 ± 0.023
2.644AspAsp: 2.644 ± 0.06
3.812AspGlu: 3.812 ± 0.065
3.789AspPhe: 3.789 ± 0.063
3.545AspGly: 3.545 ± 0.077
0.69AspHis: 0.69 ± 0.025
3.802AspIle: 3.802 ± 0.063
3.901AspLys: 3.901 ± 0.072
4.861AspLeu: 4.861 ± 0.084
1.01AspMet: 1.01 ± 0.034
3.062AspAsn: 3.062 ± 0.061
1.362AspPro: 1.362 ± 0.042
1.344AspGln: 1.344 ± 0.034
1.635AspArg: 1.635 ± 0.04
3.017AspSer: 3.017 ± 0.057
2.435AspThr: 2.435 ± 0.054
3.472AspVal: 3.472 ± 0.067
0.674AspTrp: 0.674 ± 0.027
2.654AspTyr: 2.654 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
3.952GluAla: 3.952 ± 0.074
0.402GluCys: 0.402 ± 0.025
2.996GluAsp: 2.996 ± 0.058
4.701GluGlu: 4.701 ± 0.091
3.04GluPhe: 3.04 ± 0.064
3.457GluGly: 3.457 ± 0.057
1.003GluHis: 1.003 ± 0.033
5.966GluIle: 5.966 ± 0.08
6.34GluLys: 6.34 ± 0.107
6.012GluLeu: 6.012 ± 0.092
1.6GluMet: 1.6 ± 0.046
5.266GluAsn: 5.266 ± 0.089
1.586GluPro: 1.586 ± 0.044
2.353GluGln: 2.353 ± 0.049
2.317GluArg: 2.317 ± 0.047
3.327GluSer: 3.327 ± 0.065
3.673GluThr: 3.673 ± 0.065
4.292GluVal: 4.292 ± 0.065
0.629GluTrp: 0.629 ± 0.026
2.206GluTyr: 2.206 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.226PheAla: 3.226 ± 0.06
0.495PheCys: 0.495 ± 0.026
3.473PheAsp: 3.473 ± 0.062
3.763PheGlu: 3.763 ± 0.07
3.2PhePhe: 3.2 ± 0.067
3.804PheGly: 3.804 ± 0.066
0.923PheHis: 0.923 ± 0.032
4.237PheIle: 4.237 ± 0.085
3.657PheLys: 3.657 ± 0.075
5.113PheLeu: 5.113 ± 0.092
1.182PheMet: 1.182 ± 0.04
3.552PheAsn: 3.552 ± 0.08
1.817PhePro: 1.817 ± 0.044
1.863PheGln: 1.863 ± 0.045
1.687PheArg: 1.687 ± 0.045
4.516PheSer: 4.516 ± 0.086
3.528PheThr: 3.528 ± 0.084
3.442PheVal: 3.442 ± 0.054
0.623PheTrp: 0.623 ± 0.025
2.377PheTyr: 2.377 ± 0.062
0.0PheXaa: 0.0 ± 0.0
Gly
3.956GlyAla: 3.956 ± 0.075
0.663GlyCys: 0.663 ± 0.041
2.953GlyAsp: 2.953 ± 0.061
3.373GlyGlu: 3.373 ± 0.058
3.87GlyPhe: 3.87 ± 0.071
4.467GlyGly: 4.467 ± 0.103
1.003GlyHis: 1.003 ± 0.034
5.425GlyIle: 5.425 ± 0.088
4.776GlyLys: 4.776 ± 0.085
5.576GlyLeu: 5.576 ± 0.071
1.522GlyMet: 1.522 ± 0.043
4.045GlyAsn: 4.045 ± 0.08
1.298GlyPro: 1.298 ± 0.039
1.954GlyGln: 1.954 ± 0.042
1.917GlyArg: 1.917 ± 0.045
4.098GlySer: 4.098 ± 0.079
4.447GlyThr: 4.447 ± 0.135
4.179GlyVal: 4.179 ± 0.069
0.693GlyTrp: 0.693 ± 0.034
2.582GlyTyr: 2.582 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
0.811HisAla: 0.811 ± 0.032
0.187HisCys: 0.187 ± 0.012
0.781HisAsp: 0.781 ± 0.034
0.926HisGlu: 0.926 ± 0.032
1.236HisPhe: 1.236 ± 0.035
0.916HisGly: 0.916 ± 0.03
0.453HisHis: 0.453 ± 0.025
1.265HisIle: 1.265 ± 0.037
1.117HisLys: 1.117 ± 0.035
1.687HisLeu: 1.687 ± 0.044
0.268HisMet: 0.268 ± 0.018
0.953HisAsn: 0.953 ± 0.026
0.874HisPro: 0.874 ± 0.032
0.8HisGln: 0.8 ± 0.03
0.603HisArg: 0.603 ± 0.024
1.04HisSer: 1.04 ± 0.034
0.835HisThr: 0.835 ± 0.028
0.834HisVal: 0.834 ± 0.031
0.195HisTrp: 0.195 ± 0.014
0.844HisTyr: 0.844 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.569IleAla: 5.569 ± 0.085
0.662IleCys: 0.662 ± 0.032
4.835IleAsp: 4.835 ± 0.076
5.544IleGlu: 5.544 ± 0.086
3.715IlePhe: 3.715 ± 0.07
5.142IleGly: 5.142 ± 0.083
1.388IleHis: 1.388 ± 0.039
6.322IleIle: 6.322 ± 0.106
5.706IleLys: 5.706 ± 0.1
7.171IleLeu: 7.171 ± 0.119
1.324IleMet: 1.324 ± 0.036
4.878IleAsn: 4.878 ± 0.086
3.425IlePro: 3.425 ± 0.065
3.062IleGln: 3.062 ± 0.066
2.585IleArg: 2.585 ± 0.048
5.741IleSer: 5.741 ± 0.075
5.026IleThr: 5.026 ± 0.097
5.105IleVal: 5.105 ± 0.081
0.719IleTrp: 0.719 ± 0.028
2.895IleTyr: 2.895 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.609LysAla: 4.609 ± 0.089
0.365LysCys: 0.365 ± 0.02
3.797LysAsp: 3.797 ± 0.077
5.837LysGlu: 5.837 ± 0.1
3.089LysPhe: 3.089 ± 0.065
3.987LysGly: 3.987 ± 0.077
1.168LysHis: 1.168 ± 0.038
6.742LysIle: 6.742 ± 0.112
6.903LysLys: 6.903 ± 0.129
6.439LysLeu: 6.439 ± 0.102
2.178LysMet: 2.178 ± 0.048
5.531LysAsn: 5.531 ± 0.09
2.457LysPro: 2.457 ± 0.059
2.603LysGln: 2.603 ± 0.056
2.546LysArg: 2.546 ± 0.06
4.603LysSer: 4.603 ± 0.073
4.419LysThr: 4.419 ± 0.072
4.732LysVal: 4.732 ± 0.082
0.711LysTrp: 0.711 ± 0.029
2.727LysTyr: 2.727 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
5.656LeuAla: 5.656 ± 0.068
0.676LeuCys: 0.676 ± 0.029
4.67LeuAsp: 4.67 ± 0.077
5.886LeuGlu: 5.886 ± 0.089
5.49LeuPhe: 5.49 ± 0.098
5.332LeuGly: 5.332 ± 0.067
1.578LeuHis: 1.578 ± 0.042
6.997LeuIle: 6.997 ± 0.11
7.251LeuLys: 7.251 ± 0.109
8.91LeuLeu: 8.91 ± 0.123
2.017LeuMet: 2.017 ± 0.048
5.902LeuAsn: 5.902 ± 0.085
3.553LeuPro: 3.553 ± 0.067
3.205LeuGln: 3.205 ± 0.061
2.903LeuArg: 2.903 ± 0.055
6.545LeuSer: 6.545 ± 0.088
5.428LeuThr: 5.428 ± 0.094
5.657LeuVal: 5.657 ± 0.083
0.757LeuTrp: 0.757 ± 0.028
3.118LeuTyr: 3.118 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
1.467MetAla: 1.467 ± 0.04
0.112MetCys: 0.112 ± 0.01
1.007MetAsp: 1.007 ± 0.035
1.393MetGlu: 1.393 ± 0.041
0.835MetPhe: 0.835 ± 0.031
1.341MetGly: 1.341 ± 0.041
0.386MetHis: 0.386 ± 0.02
1.722MetIle: 1.722 ± 0.046
2.262MetLys: 2.262 ± 0.045
1.826MetLeu: 1.826 ± 0.052
0.58MetMet: 0.58 ± 0.026
1.478MetAsn: 1.478 ± 0.042
0.752MetPro: 0.752 ± 0.027
0.853MetGln: 0.853 ± 0.03
0.789MetArg: 0.789 ± 0.028
1.347MetSer: 1.347 ± 0.032
1.151MetThr: 1.151 ± 0.036
1.321MetVal: 1.321 ± 0.039
0.167MetTrp: 0.167 ± 0.014
0.646MetTyr: 0.646 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.882AsnAla: 3.882 ± 0.057
0.58AsnCys: 0.58 ± 0.027
3.418AsnAsp: 3.418 ± 0.062
4.156AsnGlu: 4.156 ± 0.074
3.616AsnPhe: 3.616 ± 0.073
4.586AsnGly: 4.586 ± 0.109
1.202AsnHis: 1.202 ± 0.039
4.451AsnIle: 4.451 ± 0.069
4.181AsnLys: 4.181 ± 0.089
5.792AsnLeu: 5.792 ± 0.095
1.186AsnMet: 1.186 ± 0.031
4.335AsnAsn: 4.335 ± 0.104
3.214AsnPro: 3.214 ± 0.076
2.979AsnGln: 2.979 ± 0.069
2.218AsnArg: 2.218 ± 0.054
4.424AsnSer: 4.424 ± 0.094
3.857AsnThr: 3.857 ± 0.091
3.826AsnVal: 3.826 ± 0.078
0.867AsnTrp: 0.867 ± 0.033
3.165AsnTyr: 3.165 ± 0.072
0.0AsnXaa: 0.0 ± 0.0
Pro
2.068ProAla: 2.068 ± 0.056
0.242ProCys: 0.242 ± 0.016
1.765ProAsp: 1.765 ± 0.045
2.579ProGlu: 2.579 ± 0.049
2.164ProPhe: 2.164 ± 0.05
1.932ProGly: 1.932 ± 0.051
0.545ProHis: 0.545 ± 0.023
2.924ProIle: 2.924 ± 0.052
2.432ProLys: 2.432 ± 0.058
2.904ProLeu: 2.904 ± 0.061
0.712ProMet: 0.712 ± 0.029
2.545ProAsn: 2.545 ± 0.065
0.895ProPro: 0.895 ± 0.037
1.159ProGln: 1.159 ± 0.037
0.866ProArg: 0.866 ± 0.033
2.327ProSer: 2.327 ± 0.057
2.486ProThr: 2.486 ± 0.07
2.257ProVal: 2.257 ± 0.06
0.281ProTrp: 0.281 ± 0.017
1.304ProTyr: 1.304 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
2.033GlnAla: 2.033 ± 0.047
0.245GlnCys: 0.245 ± 0.02
1.531GlnAsp: 1.531 ± 0.038
2.209GlnGlu: 2.209 ± 0.044
2.015GlnPhe: 2.015 ± 0.047
1.796GlnGly: 1.796 ± 0.043
0.64GlnHis: 0.64 ± 0.026
2.774GlnIle: 2.774 ± 0.05
3.167GlnLys: 3.167 ± 0.072
3.694GlnLeu: 3.694 ± 0.068
0.858GlnMet: 0.858 ± 0.029
2.759GlnAsn: 2.759 ± 0.06
1.198GlnPro: 1.198 ± 0.036
1.432GlnGln: 1.432 ± 0.039
1.12GlnArg: 1.12 ± 0.032
2.197GlnSer: 2.197 ± 0.053
2.317GlnThr: 2.317 ± 0.058
2.074GlnVal: 2.074 ± 0.044
0.406GlnTrp: 0.406 ± 0.02
1.316GlnTyr: 1.316 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
1.825ArgAla: 1.825 ± 0.049
0.185ArgCys: 0.185 ± 0.014
1.569ArgAsp: 1.569 ± 0.042
2.018ArgGlu: 2.018 ± 0.053
1.939ArgPhe: 1.939 ± 0.047
1.703ArgGly: 1.703 ± 0.042
0.523ArgHis: 0.523 ± 0.022
2.812ArgIle: 2.812 ± 0.056
2.579ArgLys: 2.579 ± 0.057
3.038ArgLeu: 3.038 ± 0.054
0.953ArgMet: 0.953 ± 0.033
2.129ArgAsn: 2.129 ± 0.046
0.989ArgPro: 0.989 ± 0.031
0.96ArgGln: 0.96 ± 0.036
1.133ArgArg: 1.133 ± 0.03
1.636ArgSer: 1.636 ± 0.04
1.747ArgThr: 1.747 ± 0.048
2.032ArgVal: 2.032 ± 0.047
0.338ArgTrp: 0.338 ± 0.017
1.37ArgTyr: 1.37 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.659SerAla: 3.659 ± 0.073
0.668SerCys: 0.668 ± 0.029
3.348SerAsp: 3.348 ± 0.059
3.907SerGlu: 3.907 ± 0.062
4.265SerPhe: 4.265 ± 0.068
4.844SerGly: 4.844 ± 0.096
1.037SerHis: 1.037 ± 0.031
5.465SerIle: 5.465 ± 0.081
4.729SerLys: 4.729 ± 0.086
5.885SerLeu: 5.885 ± 0.085
1.262SerMet: 1.262 ± 0.038
4.323SerAsn: 4.323 ± 0.089
2.268SerPro: 2.268 ± 0.056
2.307SerGln: 2.307 ± 0.048
1.867SerArg: 1.867 ± 0.046
4.56SerSer: 4.56 ± 0.093
4.114SerThr: 4.114 ± 0.093
4.182SerVal: 4.182 ± 0.069
0.659SerTrp: 0.659 ± 0.028
2.64SerTyr: 2.64 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.138ThrAla: 4.138 ± 0.108
0.373ThrCys: 0.373 ± 0.025
3.055ThrAsp: 3.055 ± 0.062
3.491ThrGlu: 3.491 ± 0.069
3.555ThrPhe: 3.555 ± 0.07
4.124ThrGly: 4.124 ± 0.117
0.978ThrHis: 0.978 ± 0.03
5.621ThrIle: 5.621 ± 0.106
3.788ThrLys: 3.788 ± 0.078
5.579ThrLeu: 5.579 ± 0.098
1.008ThrMet: 1.008 ± 0.036
3.848ThrAsn: 3.848 ± 0.097
2.648ThrPro: 2.648 ± 0.076
1.998ThrGln: 1.998 ± 0.047
1.513ThrArg: 1.513 ± 0.037
4.102ThrSer: 4.102 ± 0.104
4.467ThrThr: 4.467 ± 0.155
4.407ThrVal: 4.407 ± 0.121
0.623ThrTrp: 0.623 ± 0.032
2.416ThrTyr: 2.416 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.23ValAla: 4.23 ± 0.073
0.543ValCys: 0.543 ± 0.026
3.442ValAsp: 3.442 ± 0.067
4.077ValGlu: 4.077 ± 0.057
3.636ValPhe: 3.636 ± 0.059
4.017ValGly: 4.017 ± 0.079
0.963ValHis: 0.963 ± 0.032
4.96ValIle: 4.96 ± 0.071
4.283ValLys: 4.283 ± 0.078
5.956ValLeu: 5.956 ± 0.084
1.367ValMet: 1.367 ± 0.042
3.833ValAsn: 3.833 ± 0.068
2.193ValPro: 2.193 ± 0.046
1.988ValGln: 1.988 ± 0.051
1.979ValArg: 1.979 ± 0.043
4.56ValSer: 4.56 ± 0.075
4.118ValThr: 4.118 ± 0.115
4.536ValVal: 4.536 ± 0.077
0.574ValTrp: 0.574 ± 0.027
2.512ValTyr: 2.512 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.55TrpAla: 0.55 ± 0.025
0.092TrpCys: 0.092 ± 0.009
0.509TrpAsp: 0.509 ± 0.024
0.616TrpGlu: 0.616 ± 0.023
0.624TrpPhe: 0.624 ± 0.024
0.567TrpGly: 0.567 ± 0.028
0.213TrpHis: 0.213 ± 0.015
0.754TrpIle: 0.754 ± 0.029
0.822TrpLys: 0.822 ± 0.03
0.903TrpLeu: 0.903 ± 0.033
0.275TrpMet: 0.275 ± 0.018
0.787TrpAsn: 0.787 ± 0.032
0.206TrpPro: 0.206 ± 0.014
0.436TrpGln: 0.436 ± 0.022
0.367TrpArg: 0.367 ± 0.02
0.658TrpSer: 0.658 ± 0.029
0.603TrpThr: 0.603 ± 0.033
0.592TrpVal: 0.592 ± 0.025
0.12TrpTrp: 0.12 ± 0.011
0.422TrpTyr: 0.422 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.279TyrAla: 2.279 ± 0.05
0.333TyrCys: 0.333 ± 0.019
2.09TyrAsp: 2.09 ± 0.048
2.349TyrGlu: 2.349 ± 0.044
2.617TyrPhe: 2.617 ± 0.053
2.422TyrGly: 2.422 ± 0.059
0.828TyrHis: 0.828 ± 0.035
2.693TyrIle: 2.693 ± 0.056
2.65TyrLys: 2.65 ± 0.056
3.737TyrLeu: 3.737 ± 0.068
0.64TyrMet: 0.64 ± 0.024
2.564TyrAsn: 2.564 ± 0.051
1.423TyrPro: 1.423 ± 0.035
1.759TyrGln: 1.759 ± 0.045
1.494TyrArg: 1.494 ± 0.043
2.693TyrSer: 2.693 ± 0.055
2.344TyrThr: 2.344 ± 0.07
2.241TyrVal: 2.241 ± 0.051
0.471TyrTrp: 0.471 ± 0.02
1.825TyrTyr: 1.825 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3053 proteins (1057804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski