Saprospira grandis (strain Lewin)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Saprospiraceae; Saprospira; Saprospira grandis

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6KZG7|H6KZG7_SAPGL Membrane protein OS=Saprospira grandis (strain Lewin) OX=984262 GN=SGRA_3015 PE=4 SV=1
MM1 pKa = 7.54FNQKK5 pKa = 8.05LTQVLVALAFLLSAGSLSAQTVLWGAGSSDD35 pKa = 3.58TQIDD39 pKa = 4.04SIGRR43 pKa = 11.84FATDD47 pKa = 3.78TIIGLDD53 pKa = 3.63GLGWTVDD60 pKa = 3.49SVAGKK65 pKa = 9.31WEE67 pKa = 3.87YY68 pKa = 11.25SVDD71 pKa = 4.32GISQGTFSSGTPGLTSPSVGDD92 pKa = 3.52GVAFFDD98 pKa = 3.95ADD100 pKa = 3.31FWFAQGANPQIGTITSPAFDD120 pKa = 3.39LTGYY124 pKa = 9.73EE125 pKa = 4.01DD126 pKa = 4.18SIVVLEE132 pKa = 5.34FYY134 pKa = 11.0AGLRR138 pKa = 11.84DD139 pKa = 4.78FRR141 pKa = 11.84STDD144 pKa = 2.9NSAGFSTDD152 pKa = 5.0GGATWTDD159 pKa = 3.68FDD161 pKa = 4.63VISAIGAPSGSAGEE175 pKa = 4.2GMVLFDD181 pKa = 5.29LSNIVNSATSLTNCHH196 pKa = 5.2VRR198 pKa = 11.84FSFEE202 pKa = 3.61GDD204 pKa = 2.95SYY206 pKa = 11.61YY207 pKa = 11.25YY208 pKa = 10.92GVDD211 pKa = 3.29DD212 pKa = 3.75VTVRR216 pKa = 11.84TSEE219 pKa = 4.46AIYY222 pKa = 10.48DD223 pKa = 3.53IAIDD227 pKa = 3.85EE228 pKa = 4.76NYY230 pKa = 10.55NPPKK234 pKa = 10.71VVGSKK239 pKa = 9.49QLPLRR244 pKa = 11.84MVNADD249 pKa = 3.36EE250 pKa = 4.72MGWGANIEE258 pKa = 4.21NNGIGGNINAGEE270 pKa = 4.03AMLYY274 pKa = 8.33VTVSEE279 pKa = 4.51VSSGTVAVMDD289 pKa = 4.24SMAVPAIAPGTDD301 pKa = 2.77TALYY305 pKa = 9.47MPGFSNGWMPTAAGDD320 pKa = 3.67YY321 pKa = 9.99RR322 pKa = 11.84VDD324 pKa = 3.54YY325 pKa = 10.65SVSYY329 pKa = 9.28SANMQIDD336 pKa = 3.9TTNDD340 pKa = 2.68VYY342 pKa = 11.55TDD344 pKa = 4.23FFTITADD351 pKa = 3.72DD352 pKa = 4.03YY353 pKa = 11.09LSKK356 pKa = 10.76VGRR359 pKa = 11.84DD360 pKa = 3.25LTGFPSADD368 pKa = 3.25AATLPGVRR376 pKa = 11.84AADD379 pKa = 3.41GFLPAEE385 pKa = 4.47HH386 pKa = 7.0EE387 pKa = 4.37WGSMYY392 pKa = 10.31YY393 pKa = 9.68IPNYY397 pKa = 9.42TVTNGDD403 pKa = 4.11SIVADD408 pKa = 3.35SFLYY412 pKa = 10.25TGATVNADD420 pKa = 3.5TSVTEE425 pKa = 3.9AVVQVRR431 pKa = 11.84LYY433 pKa = 10.97KK434 pKa = 10.95FNDD437 pKa = 3.51DD438 pKa = 4.51DD439 pKa = 6.38GSGFWTAQPTGPSDD453 pKa = 3.73PEE455 pKa = 4.15LPIQALGVDD464 pKa = 4.04TFEE467 pKa = 6.34LDD469 pKa = 2.85LSLAFRR475 pKa = 11.84QRR477 pKa = 11.84AVALSNLSGGTGAVLQPNSFYY498 pKa = 11.19VATVLCSDD506 pKa = 4.58QINGLRR512 pKa = 11.84NADD515 pKa = 3.15NTTRR519 pKa = 11.84HH520 pKa = 5.39IFIGNSEE527 pKa = 4.45DD528 pKa = 4.74KK529 pKa = 11.19YY530 pKa = 11.58DD531 pKa = 3.73FTIDD535 pKa = 3.38SLPNYY540 pKa = 7.64FIPASIVRR548 pKa = 11.84SAYY551 pKa = 9.3IDD553 pKa = 3.33PTGTVTGLQDD563 pKa = 3.73NQWYY567 pKa = 8.39GAGYY571 pKa = 9.98EE572 pKa = 4.15SGIVPSIGLTLTDD585 pKa = 5.55AITINVTANEE595 pKa = 3.99AVTTEE600 pKa = 4.41FNIFPNPATEE610 pKa = 4.05QISAKK615 pKa = 10.52VEE617 pKa = 3.95LTEE620 pKa = 4.02MNSNVEE626 pKa = 4.13YY627 pKa = 10.33IITDD631 pKa = 3.08ATGRR635 pKa = 11.84VVIKK639 pKa = 8.8EE640 pKa = 4.01QKK642 pKa = 10.67ADD644 pKa = 3.67LQSDD648 pKa = 4.2VVTYY652 pKa = 10.52DD653 pKa = 3.19VSQLPAGVYY662 pKa = 9.43FFTVRR667 pKa = 11.84TAQGASSQKK676 pKa = 9.9FVKK679 pKa = 10.06QQ680 pKa = 3.29

Molecular weight: 72.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6L936|H6L936_SAPGL Uncharacterized protein OS=Saprospira grandis (strain Lewin) OX=984262 GN=SGRA_0433 PE=4 SV=1
MM1 pKa = 7.48SGSKK5 pKa = 10.0LVRR8 pKa = 11.84SGGKK12 pKa = 9.25LVRR15 pKa = 11.84SGGKK19 pKa = 9.25LVRR22 pKa = 11.84SGGKK26 pKa = 9.25LVRR29 pKa = 11.84SGGKK33 pKa = 9.27LVRR36 pKa = 11.84SGSKK40 pKa = 9.93LVRR43 pKa = 11.84FGGKK47 pKa = 9.63LVMSGGKK54 pKa = 9.25LVRR57 pKa = 11.84SGGKK61 pKa = 9.27LVRR64 pKa = 11.84SGSKK68 pKa = 10.53LVMSGSKK75 pKa = 10.0LVRR78 pKa = 11.84SGGKK82 pKa = 9.83LVMSGSKK89 pKa = 10.0LVRR92 pKa = 11.84SGGKK96 pKa = 9.19LVRR99 pKa = 11.84FGGKK103 pKa = 9.36LVMPIVAA110 pKa = 4.7

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4218

0

4218

1241634

30

3619

294.4

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.746 ± 0.054

1.016 ± 0.021

4.9 ± 0.035

6.991 ± 0.055

4.8 ± 0.033

6.647 ± 0.049

1.693 ± 0.021

5.636 ± 0.033

6.348 ± 0.071

11.369 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.022

4.263 ± 0.038

4.27 ± 0.029

5.409 ± 0.042

4.744 ± 0.042

6.46 ± 0.045

4.051 ± 0.071

5.033 ± 0.04

1.389 ± 0.018

4.018 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski