Sporosarcina pasteurii (Bacillus pasteurii)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A380BJH9|A0A380BJH9_SPOPA Glycerol-3-phosphate dehydrogenase OS=Sporosarcina pasteurii OX=1474 GN=glpD PE=3 SV=1
MM1 pKa = 7.31LRR3 pKa = 11.84SKK5 pKa = 10.8KK6 pKa = 9.96RR7 pKa = 11.84GKK9 pKa = 9.1TYY11 pKa = 9.12MPKK14 pKa = 9.11YY15 pKa = 9.1TIVDD19 pKa = 3.32QDD21 pKa = 3.54TCIACGACGAAAPDD35 pKa = 3.53IYY37 pKa = 10.99DD38 pKa = 3.8YY39 pKa = 11.65DD40 pKa = 4.89DD41 pKa = 5.12DD42 pKa = 6.45GIAFVILDD50 pKa = 4.45DD51 pKa = 3.86NTGTVQVPEE60 pKa = 4.18EE61 pKa = 4.25LLEE64 pKa = 4.41DD65 pKa = 3.78MEE67 pKa = 6.6DD68 pKa = 3.82GFEE71 pKa = 4.27GCPTDD76 pKa = 3.92SIKK79 pKa = 11.12VADD82 pKa = 4.41APFDD86 pKa = 4.06GDD88 pKa = 3.54ALKK91 pKa = 11.37YY92 pKa = 10.52EE93 pKa = 4.55DD94 pKa = 4.06

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A380CK83|A0A380CK83_SPOPA 50S ribosomal protein L30 OS=Sporosarcina pasteurii OX=1474 GN=rpmD PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.08RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84KK12 pKa = 9.73RR13 pKa = 11.84SKK15 pKa = 9.74VHH17 pKa = 5.79GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 9.53SGRR29 pKa = 11.84RR30 pKa = 11.84IIAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.83GRR40 pKa = 11.84KK41 pKa = 8.75VLSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3142

0

3142

927722

30

1727

295.3

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.536 ± 0.047

0.652 ± 0.017

5.12 ± 0.033

7.815 ± 0.047

4.531 ± 0.035

7.05 ± 0.042

2.104 ± 0.021

7.98 ± 0.037

6.23 ± 0.036

9.57 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.913 ± 0.02

4.185 ± 0.033

3.651 ± 0.023

3.505 ± 0.027

4.221 ± 0.032

5.756 ± 0.028

5.68 ± 0.023

7.253 ± 0.03

0.965 ± 0.015

3.282 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski