Amino acid dipepetide frequency for Escherichia phage vB_EcoS_HASG4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.262AlaAla: 7.262 ± 1.051
0.669AlaCys: 0.669 ± 0.143
3.886AlaAsp: 3.886 ± 0.363
5.064AlaGlu: 5.064 ± 0.387
3.28AlaPhe: 3.28 ± 0.291
5.064AlaGly: 5.064 ± 0.578
1.465AlaHis: 1.465 ± 0.252
4.618AlaIle: 4.618 ± 0.411
6.784AlaLys: 6.784 ± 0.5
6.529AlaLeu: 6.529 ± 0.469
2.102AlaMet: 2.102 ± 0.291
3.28AlaAsn: 3.28 ± 0.367
2.548AlaPro: 2.548 ± 0.305
3.472AlaGln: 3.472 ± 0.358
2.994AlaArg: 2.994 ± 0.29
5.0AlaSer: 5.0 ± 0.683
3.822AlaThr: 3.822 ± 0.441
4.714AlaVal: 4.714 ± 0.411
0.796AlaTrp: 0.796 ± 0.16
2.771AlaTyr: 2.771 ± 0.252
0.0AlaXaa: 0.0 ± 0.0
Cys
0.51CysAla: 0.51 ± 0.13
0.255CysCys: 0.255 ± 0.109
0.478CysAsp: 0.478 ± 0.119
0.796CysGlu: 0.796 ± 0.162
0.478CysPhe: 0.478 ± 0.111
0.924CysGly: 0.924 ± 0.206
0.382CysHis: 0.382 ± 0.099
0.669CysIle: 0.669 ± 0.144
0.796CysLys: 0.796 ± 0.162
0.828CysLeu: 0.828 ± 0.15
0.255CysMet: 0.255 ± 0.099
0.541CysAsn: 0.541 ± 0.136
0.478CysPro: 0.478 ± 0.141
0.478CysGln: 0.478 ± 0.136
0.573CysArg: 0.573 ± 0.14
0.892CysSer: 0.892 ± 0.145
0.541CysThr: 0.541 ± 0.132
0.414CysVal: 0.414 ± 0.157
0.127CysTrp: 0.127 ± 0.064
0.382CysTyr: 0.382 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.395AspAla: 4.395 ± 0.332
0.637AspCys: 0.637 ± 0.159
3.058AspAsp: 3.058 ± 0.388
4.809AspGlu: 4.809 ± 0.441
2.643AspPhe: 2.643 ± 0.314
3.917AspGly: 3.917 ± 0.305
1.083AspHis: 1.083 ± 0.173
4.236AspIle: 4.236 ± 0.43
4.3AspLys: 4.3 ± 0.431
5.191AspLeu: 5.191 ± 0.376
1.879AspMet: 1.879 ± 0.238
2.739AspAsn: 2.739 ± 0.297
2.612AspPro: 2.612 ± 0.217
1.624AspGln: 1.624 ± 0.213
2.293AspArg: 2.293 ± 0.324
3.535AspSer: 3.535 ± 0.321
3.949AspThr: 3.949 ± 0.339
3.408AspVal: 3.408 ± 0.351
0.764AspTrp: 0.764 ± 0.162
2.643AspTyr: 2.643 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
5.701GluAla: 5.701 ± 0.454
0.605GluCys: 0.605 ± 0.131
3.503GluAsp: 3.503 ± 0.345
4.873GluGlu: 4.873 ± 0.551
2.548GluPhe: 2.548 ± 0.276
4.077GluGly: 4.077 ± 0.339
1.21GluHis: 1.21 ± 0.195
5.287GluIle: 5.287 ± 0.41
5.032GluLys: 5.032 ± 0.415
7.039GluLeu: 7.039 ± 0.519
2.452GluMet: 2.452 ± 0.285
2.898GluAsn: 2.898 ± 0.346
1.433GluPro: 1.433 ± 0.242
3.185GluGln: 3.185 ± 0.365
3.089GluArg: 3.089 ± 0.344
3.408GluSer: 3.408 ± 0.345
3.917GluThr: 3.917 ± 0.314
4.65GluVal: 4.65 ± 0.349
1.115GluTrp: 1.115 ± 0.186
3.026GluTyr: 3.026 ± 0.35
0.0GluXaa: 0.0 ± 0.0
Phe
2.675PheAla: 2.675 ± 0.285
0.287PheCys: 0.287 ± 0.12
2.803PheAsp: 2.803 ± 0.38
2.293PheGlu: 2.293 ± 0.275
1.37PhePhe: 1.37 ± 0.207
2.994PheGly: 2.994 ± 0.384
1.178PheHis: 1.178 ± 0.236
2.421PheIle: 2.421 ± 0.288
2.962PheLys: 2.962 ± 0.282
3.376PheLeu: 3.376 ± 0.301
0.733PheMet: 0.733 ± 0.131
2.803PheAsn: 2.803 ± 0.313
1.561PhePro: 1.561 ± 0.206
1.051PheGln: 1.051 ± 0.159
1.752PheArg: 1.752 ± 0.295
3.376PheSer: 3.376 ± 0.355
2.389PheThr: 2.389 ± 0.289
2.771PheVal: 2.771 ± 0.268
0.541PheTrp: 0.541 ± 0.154
1.847PheTyr: 1.847 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
4.523GlyAla: 4.523 ± 0.57
0.987GlyCys: 0.987 ± 0.21
3.599GlyAsp: 3.599 ± 0.308
4.427GlyGlu: 4.427 ± 0.39
3.472GlyPhe: 3.472 ± 0.304
3.28GlyGly: 3.28 ± 0.407
1.083GlyHis: 1.083 ± 0.211
4.459GlyIle: 4.459 ± 0.377
5.383GlyLys: 5.383 ± 0.481
4.777GlyLeu: 4.777 ± 0.444
1.815GlyMet: 1.815 ± 0.258
3.472GlyAsn: 3.472 ± 0.419
1.083GlyPro: 1.083 ± 0.173
2.038GlyGln: 2.038 ± 0.251
2.771GlyArg: 2.771 ± 0.282
4.077GlySer: 4.077 ± 0.387
3.408GlyThr: 3.408 ± 0.391
4.65GlyVal: 4.65 ± 0.434
0.955GlyTrp: 0.955 ± 0.169
3.121GlyTyr: 3.121 ± 0.314
0.0GlyXaa: 0.0 ± 0.0
His
1.147HisAla: 1.147 ± 0.24
0.35HisCys: 0.35 ± 0.125
1.465HisAsp: 1.465 ± 0.216
1.147HisGlu: 1.147 ± 0.207
0.637HisPhe: 0.637 ± 0.175
1.306HisGly: 1.306 ± 0.218
0.51HisHis: 0.51 ± 0.154
1.37HisIle: 1.37 ± 0.21
1.401HisLys: 1.401 ± 0.191
1.815HisLeu: 1.815 ± 0.284
0.382HisMet: 0.382 ± 0.131
0.892HisAsn: 0.892 ± 0.209
0.987HisPro: 0.987 ± 0.209
0.541HisGln: 0.541 ± 0.132
1.21HisArg: 1.21 ± 0.197
0.987HisSer: 0.987 ± 0.201
0.701HisThr: 0.701 ± 0.15
0.796HisVal: 0.796 ± 0.153
0.096HisTrp: 0.096 ± 0.054
0.669HisTyr: 0.669 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
5.446IleAla: 5.446 ± 0.364
0.892IleCys: 0.892 ± 0.181
4.746IleAsp: 4.746 ± 0.437
4.427IleGlu: 4.427 ± 0.39
2.198IlePhe: 2.198 ± 0.248
4.109IleGly: 4.109 ± 0.373
1.115IleHis: 1.115 ± 0.171
4.268IleIle: 4.268 ± 0.345
4.586IleLys: 4.586 ± 0.484
5.637IleLeu: 5.637 ± 0.42
1.688IleMet: 1.688 ± 0.257
4.109IleAsn: 4.109 ± 0.371
2.898IlePro: 2.898 ± 0.357
2.07IleGln: 2.07 ± 0.281
2.675IleArg: 2.675 ± 0.321
4.491IleSer: 4.491 ± 0.356
4.3IleThr: 4.3 ± 0.342
3.472IleVal: 3.472 ± 0.333
0.573IleTrp: 0.573 ± 0.148
2.261IleTyr: 2.261 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
6.306LysAla: 6.306 ± 0.539
0.51LysCys: 0.51 ± 0.135
4.491LysAsp: 4.491 ± 0.423
5.128LysGlu: 5.128 ± 0.415
3.344LysPhe: 3.344 ± 0.33
3.089LysGly: 3.089 ± 0.333
1.242LysHis: 1.242 ± 0.218
4.109LysIle: 4.109 ± 0.433
4.427LysLys: 4.427 ± 0.462
6.784LysLeu: 6.784 ± 0.593
2.134LysMet: 2.134 ± 0.29
3.631LysAsn: 3.631 ± 0.428
2.421LysPro: 2.421 ± 0.294
3.344LysGln: 3.344 ± 0.306
3.854LysArg: 3.854 ± 0.357
4.554LysSer: 4.554 ± 0.426
4.427LysThr: 4.427 ± 0.369
4.714LysVal: 4.714 ± 0.443
0.637LysTrp: 0.637 ± 0.171
3.185LysTyr: 3.185 ± 0.282
0.0LysXaa: 0.0 ± 0.0
Leu
7.102LeuAla: 7.102 ± 0.525
0.764LeuCys: 0.764 ± 0.138
6.179LeuAsp: 6.179 ± 0.476
7.134LeuGlu: 7.134 ± 0.544
2.771LeuPhe: 2.771 ± 0.348
5.096LeuGly: 5.096 ± 0.464
1.847LeuHis: 1.847 ± 0.311
5.351LeuIle: 5.351 ± 0.432
5.701LeuLys: 5.701 ± 0.314
6.465LeuLeu: 6.465 ± 0.493
2.325LeuMet: 2.325 ± 0.269
5.319LeuAsn: 5.319 ± 0.438
3.408LeuPro: 3.408 ± 0.389
3.344LeuGln: 3.344 ± 0.4
3.886LeuArg: 3.886 ± 0.285
5.51LeuSer: 5.51 ± 0.447
5.0LeuThr: 5.0 ± 0.429
4.968LeuVal: 4.968 ± 0.474
0.701LeuTrp: 0.701 ± 0.178
2.643LeuTyr: 2.643 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
1.688MetAla: 1.688 ± 0.261
0.223MetCys: 0.223 ± 0.078
1.147MetAsp: 1.147 ± 0.193
1.975MetGlu: 1.975 ± 0.308
0.892MetPhe: 0.892 ± 0.179
1.592MetGly: 1.592 ± 0.2
0.478MetHis: 0.478 ± 0.139
2.102MetIle: 2.102 ± 0.303
2.612MetLys: 2.612 ± 0.353
2.293MetLeu: 2.293 ± 0.352
0.637MetMet: 0.637 ± 0.138
1.051MetAsn: 1.051 ± 0.191
0.987MetPro: 0.987 ± 0.161
1.433MetGln: 1.433 ± 0.3
1.115MetArg: 1.115 ± 0.171
2.229MetSer: 2.229 ± 0.305
1.911MetThr: 1.911 ± 0.196
1.338MetVal: 1.338 ± 0.211
0.191MetTrp: 0.191 ± 0.085
0.955MetTyr: 0.955 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.949AsnAla: 3.949 ± 0.585
0.541AsnCys: 0.541 ± 0.152
2.58AsnAsp: 2.58 ± 0.282
2.898AsnGlu: 2.898 ± 0.336
2.421AsnPhe: 2.421 ± 0.327
4.554AsnGly: 4.554 ± 0.531
0.828AsnHis: 0.828 ± 0.159
3.631AsnIle: 3.631 ± 0.316
3.631AsnLys: 3.631 ± 0.338
4.682AsnLeu: 4.682 ± 0.435
1.21AsnMet: 1.21 ± 0.17
3.376AsnAsn: 3.376 ± 0.374
2.325AsnPro: 2.325 ± 0.228
1.752AsnGln: 1.752 ± 0.322
2.452AsnArg: 2.452 ± 0.302
3.472AsnSer: 3.472 ± 0.404
3.567AsnThr: 3.567 ± 0.337
3.631AsnVal: 3.631 ± 0.361
0.573AsnTrp: 0.573 ± 0.153
1.847AsnTyr: 1.847 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
2.643ProAla: 2.643 ± 0.281
0.382ProCys: 0.382 ± 0.103
2.58ProAsp: 2.58 ± 0.285
3.185ProGlu: 3.185 ± 0.351
1.624ProPhe: 1.624 ± 0.236
2.166ProGly: 2.166 ± 0.274
0.573ProHis: 0.573 ± 0.13
2.452ProIle: 2.452 ± 0.281
1.847ProLys: 1.847 ± 0.244
2.357ProLeu: 2.357 ± 0.281
0.541ProMet: 0.541 ± 0.128
1.943ProAsn: 1.943 ± 0.252
1.338ProPro: 1.338 ± 0.299
0.955ProGln: 0.955 ± 0.203
1.497ProArg: 1.497 ± 0.222
2.102ProSer: 2.102 ± 0.273
2.006ProThr: 2.006 ± 0.242
2.643ProVal: 2.643 ± 0.257
0.446ProTrp: 0.446 ± 0.117
1.784ProTyr: 1.784 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
2.771GlnAla: 2.771 ± 0.467
0.446GlnCys: 0.446 ± 0.11
1.911GlnAsp: 1.911 ± 0.257
2.835GlnGlu: 2.835 ± 0.297
1.592GlnPhe: 1.592 ± 0.212
1.752GlnGly: 1.752 ± 0.212
0.764GlnHis: 0.764 ± 0.174
2.643GlnIle: 2.643 ± 0.304
2.421GlnLys: 2.421 ± 0.344
4.109GlnLeu: 4.109 ± 0.42
1.21GlnMet: 1.21 ± 0.284
1.815GlnAsn: 1.815 ± 0.237
0.733GlnPro: 0.733 ± 0.177
2.675GlnGln: 2.675 ± 0.569
1.847GlnArg: 1.847 ± 0.252
2.357GlnSer: 2.357 ± 0.324
2.102GlnThr: 2.102 ± 0.276
2.803GlnVal: 2.803 ± 0.323
0.573GlnTrp: 0.573 ± 0.141
1.401GlnTyr: 1.401 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
3.153ArgAla: 3.153 ± 0.297
0.35ArgCys: 0.35 ± 0.11
2.771ArgAsp: 2.771 ± 0.238
2.994ArgGlu: 2.994 ± 0.347
1.656ArgPhe: 1.656 ± 0.263
3.153ArgGly: 3.153 ± 0.273
0.541ArgHis: 0.541 ± 0.15
2.866ArgIle: 2.866 ± 0.331
3.121ArgLys: 3.121 ± 0.397
4.045ArgLeu: 4.045 ± 0.349
1.529ArgMet: 1.529 ± 0.239
2.643ArgAsn: 2.643 ± 0.27
1.433ArgPro: 1.433 ± 0.154
1.752ArgGln: 1.752 ± 0.236
2.325ArgArg: 2.325 ± 0.282
2.548ArgSer: 2.548 ± 0.291
2.452ArgThr: 2.452 ± 0.261
2.898ArgVal: 2.898 ± 0.323
0.478ArgTrp: 0.478 ± 0.12
1.752ArgTyr: 1.752 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
4.65SerAla: 4.65 ± 0.644
0.796SerCys: 0.796 ± 0.217
3.344SerAsp: 3.344 ± 0.349
3.981SerGlu: 3.981 ± 0.49
3.058SerPhe: 3.058 ± 0.267
5.0SerGly: 5.0 ± 0.431
0.955SerHis: 0.955 ± 0.175
4.013SerIle: 4.013 ± 0.39
4.873SerLys: 4.873 ± 0.43
5.988SerLeu: 5.988 ± 0.426
1.529SerMet: 1.529 ± 0.216
3.631SerAsn: 3.631 ± 0.412
1.975SerPro: 1.975 ± 0.258
2.261SerGln: 2.261 ± 0.318
2.771SerArg: 2.771 ± 0.339
4.363SerSer: 4.363 ± 0.432
3.726SerThr: 3.726 ± 0.334
4.491SerVal: 4.491 ± 0.353
1.083SerTrp: 1.083 ± 0.186
2.293SerTyr: 2.293 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
4.331ThrAla: 4.331 ± 0.608
0.605ThrCys: 0.605 ± 0.147
3.153ThrAsp: 3.153 ± 0.294
3.249ThrGlu: 3.249 ± 0.298
2.484ThrPhe: 2.484 ± 0.277
4.714ThrGly: 4.714 ± 0.403
0.892ThrHis: 0.892 ± 0.176
4.077ThrIle: 4.077 ± 0.382
3.663ThrLys: 3.663 ± 0.348
4.554ThrLeu: 4.554 ± 0.377
1.497ThrMet: 1.497 ± 0.225
3.249ThrAsn: 3.249 ± 0.336
2.548ThrPro: 2.548 ± 0.276
2.357ThrGln: 2.357 ± 0.266
2.739ThrArg: 2.739 ± 0.313
3.949ThrSer: 3.949 ± 0.435
3.28ThrThr: 3.28 ± 0.285
4.3ThrVal: 4.3 ± 0.446
0.764ThrTrp: 0.764 ± 0.183
2.261ThrTyr: 2.261 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
4.937ValAla: 4.937 ± 0.442
0.573ValCys: 0.573 ± 0.167
4.363ValAsp: 4.363 ± 0.503
4.363ValGlu: 4.363 ± 0.412
2.484ValPhe: 2.484 ± 0.33
3.44ValGly: 3.44 ± 0.394
1.019ValHis: 1.019 ± 0.201
4.682ValIle: 4.682 ± 0.335
4.809ValLys: 4.809 ± 0.397
5.064ValLeu: 5.064 ± 0.343
1.561ValMet: 1.561 ± 0.201
3.312ValAsn: 3.312 ± 0.407
2.643ValPro: 2.643 ± 0.329
2.261ValGln: 2.261 ± 0.305
2.516ValArg: 2.516 ± 0.276
4.523ValSer: 4.523 ± 0.403
4.045ValThr: 4.045 ± 0.372
4.109ValVal: 4.109 ± 0.36
0.669ValTrp: 0.669 ± 0.167
2.452ValTyr: 2.452 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
0.541TrpAla: 0.541 ± 0.152
0.255TrpCys: 0.255 ± 0.089
0.828TrpAsp: 0.828 ± 0.173
1.147TrpGlu: 1.147 ± 0.202
0.541TrpPhe: 0.541 ± 0.148
0.669TrpGly: 0.669 ± 0.165
0.191TrpHis: 0.191 ± 0.093
0.541TrpIle: 0.541 ± 0.142
0.987TrpLys: 0.987 ± 0.161
1.083TrpLeu: 1.083 ± 0.178
0.35TrpMet: 0.35 ± 0.105
0.701TrpAsn: 0.701 ± 0.173
0.159TrpPro: 0.159 ± 0.091
0.51TrpGln: 0.51 ± 0.122
0.541TrpArg: 0.541 ± 0.108
0.764TrpSer: 0.764 ± 0.19
0.541TrpThr: 0.541 ± 0.116
0.764TrpVal: 0.764 ± 0.137
0.191TrpTrp: 0.191 ± 0.105
0.478TrpTyr: 0.478 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.389TyrAla: 2.389 ± 0.315
0.669TyrCys: 0.669 ± 0.139
2.835TyrAsp: 2.835 ± 0.327
2.325TyrGlu: 2.325 ± 0.259
1.688TyrPhe: 1.688 ± 0.263
2.484TyrGly: 2.484 ± 0.304
1.083TyrHis: 1.083 ± 0.153
2.166TyrIle: 2.166 ± 0.267
2.994TyrLys: 2.994 ± 0.325
2.898TyrLeu: 2.898 ± 0.342
0.987TyrMet: 0.987 ± 0.164
2.516TyrAsn: 2.516 ± 0.289
1.497TyrPro: 1.497 ± 0.203
1.656TyrGln: 1.656 ± 0.229
1.561TyrArg: 1.561 ± 0.224
2.643TyrSer: 2.643 ± 0.267
2.58TyrThr: 2.58 ± 0.244
2.293TyrVal: 2.293 ± 0.291
0.51TyrTrp: 0.51 ± 0.142
1.656TyrTyr: 1.656 ± 0.234
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 161 proteins (31399 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski