Trifolium medium

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 116147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A392QUT1|A0A392QUT1_9FABA Calmodulin protein kinase OS=Trifolium medium OX=97028 PE=4 SV=1
MM1 pKa = 8.49DD2 pKa = 4.0SQQNNLFDD10 pKa = 3.9TASQPDD16 pKa = 4.14TGNDD20 pKa = 3.02AYY22 pKa = 10.11TFLEE26 pKa = 4.74FNTQGEE32 pKa = 4.36DD33 pKa = 2.85FDD35 pKa = 4.65YY36 pKa = 11.39PEE38 pKa = 4.26FRR40 pKa = 11.84DD41 pKa = 4.24PIRR44 pKa = 11.84SSVAWPTPSDD54 pKa = 3.72SLADD58 pKa = 3.84PSXXXXXXXXXXXXXXXXXXXXXXXXXXGRR88 pKa = 11.84SGSGSQIVDD97 pKa = 3.39SLAAGMSGLNFEE109 pKa = 5.2DD110 pKa = 4.21TGDD113 pKa = 3.78DD114 pKa = 3.79DD115 pKa = 5.21NYY117 pKa = 10.94EE118 pKa = 4.07FGKK121 pKa = 11.2GDD123 pKa = 3.79FTEE126 pKa = 4.59HH127 pKa = 6.33ACRR130 pKa = 11.84YY131 pKa = 9.49CGVSNPACVVRR142 pKa = 11.84CNVPSS147 pKa = 3.42

Molecular weight: 13.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A392Q6B9|A0A392Q6B9_9FABA Uncharacterized protein OS=Trifolium medium OX=97028 PE=4 SV=1
GG1 pKa = 5.99QASGGRR7 pKa = 11.84GGGRR11 pKa = 11.84GGGGFGRR18 pKa = 11.84GGGRR22 pKa = 11.84GFRR25 pKa = 11.84GRR27 pKa = 11.84GPPRR31 pKa = 11.84GGRR34 pKa = 11.84GPPRR38 pKa = 11.84GGRR41 pKa = 11.84GFRR44 pKa = 11.84GRR46 pKa = 11.84GRR48 pKa = 11.84FF49 pKa = 3.35

Molecular weight: 4.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

116147

0

116147

11926644

8

2652

102.7

11.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.679 ± 0.009

1.791 ± 0.005

5.586 ± 0.009

7.144 ± 0.013

3.905 ± 0.008

6.535 ± 0.011

2.335 ± 0.005

5.048 ± 0.008

6.455 ± 0.011

8.948 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.006

4.412 ± 0.008

4.932 ± 0.012

3.745 ± 0.008

5.447 ± 0.009

8.108 ± 0.011

4.919 ± 0.009

6.924 ± 0.011

1.44 ± 0.005

2.483 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski