Paracoccus subflavus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9FXL5|A0A4Q9FXL5_9RHOB Class I SAM-dependent methyltransferase OS=Paracoccus subflavus OX=2528244 GN=EYE42_14230 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.26SPKK6 pKa = 8.77TAALLIILAAGPAMAQTPATQPQDD30 pKa = 2.88AAAASQPLGLGRR42 pKa = 11.84PALPEE47 pKa = 3.94EE48 pKa = 4.22VAAWNLDD55 pKa = 3.33VSPDD59 pKa = 3.49GTGLPEE65 pKa = 4.24GSGDD69 pKa = 3.45VMTGEE74 pKa = 4.02EE75 pKa = 4.92VYY77 pKa = 11.07LEE79 pKa = 4.13NCAACHH85 pKa = 6.32GDD87 pKa = 3.48FAEE90 pKa = 6.23GLDD93 pKa = 3.9NWPSLAGGEE102 pKa = 4.39GTLDD106 pKa = 3.23QQNPEE111 pKa = 4.09KK112 pKa = 10.31TIGSYY117 pKa = 8.64WPYY120 pKa = 11.18LSTVWDD126 pKa = 3.88YY127 pKa = 9.93VHH129 pKa = 7.42RR130 pKa = 11.84SMPFGNAQILSADD143 pKa = 3.47DD144 pKa = 3.56TYY146 pKa = 11.84AIVAYY151 pKa = 10.24LLYY154 pKa = 11.05SNALVEE160 pKa = 4.8DD161 pKa = 4.34DD162 pKa = 4.51FVLSRR167 pKa = 11.84EE168 pKa = 4.14NFTEE172 pKa = 4.07VQLPNVDD179 pKa = 3.88GFIPDD184 pKa = 3.65DD185 pKa = 4.17RR186 pKa = 11.84EE187 pKa = 3.83EE188 pKa = 4.18TEE190 pKa = 4.09YY191 pKa = 11.33GVFSAEE197 pKa = 3.66PCMEE201 pKa = 3.97NCKK204 pKa = 10.46EE205 pKa = 4.18SVEE208 pKa = 4.19VTMRR212 pKa = 11.84ASVLDD217 pKa = 3.81VTPGSSDD224 pKa = 3.29DD225 pKa = 3.6AAPAEE230 pKa = 4.35EE231 pKa = 5.37GEE233 pKa = 4.55DD234 pKa = 3.51AAEE237 pKa = 4.59DD238 pKa = 3.52VGLNN242 pKa = 3.53

Molecular weight: 25.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9G3D8|A0A4Q9G3D8_9RHOB Precorrin-2 C(20)-methyltransferase OS=Paracoccus subflavus OX=2528244 GN=EYE42_03260 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39GGQLVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3059

0

3059

961796

28

2123

314.4

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.126 ± 0.064

0.834 ± 0.015

6.312 ± 0.036

5.166 ± 0.033

3.458 ± 0.025

9.0 ± 0.041

2.087 ± 0.022

5.073 ± 0.033

2.398 ± 0.036

10.231 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.021

2.326 ± 0.02

5.568 ± 0.042

3.298 ± 0.024

7.75 ± 0.043

4.754 ± 0.026

5.212 ± 0.027

7.204 ± 0.034

1.457 ± 0.017

1.995 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski