Eubacteriaceae bacterium CHKCI005

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; unclassified Eubacteriaceae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143ZXI7|A0A143ZXI7_9FIRM Uncharacterized protein OS=Eubacteriaceae bacterium CHKCI005 OX=1780381 GN=BN3661_01470 PE=4 SV=1
MM1 pKa = 7.14STHH4 pKa = 5.64KK5 pKa = 10.23HH6 pKa = 5.71INTICCISLVAVLLITILFLGFGSTGIVAVSKK38 pKa = 10.77ALGYY42 pKa = 8.58EE43 pKa = 3.71EE44 pKa = 5.74RR45 pKa = 11.84LFDD48 pKa = 3.51TSRR51 pKa = 11.84VHH53 pKa = 6.27TLDD56 pKa = 3.04IVMDD60 pKa = 3.52NWDD63 pKa = 4.13EE64 pKa = 4.96FVDD67 pKa = 3.47NCQNEE72 pKa = 4.81EE73 pKa = 4.25YY74 pKa = 10.31VSCSVVIDD82 pKa = 3.57NEE84 pKa = 4.27SFKK87 pKa = 11.29NVAIRR92 pKa = 11.84AKK94 pKa = 10.67GNTSLSSVAAYY105 pKa = 10.51GNDD108 pKa = 3.09RR109 pKa = 11.84YY110 pKa = 11.01SFKK113 pKa = 10.67IEE115 pKa = 3.6FDD117 pKa = 3.18HH118 pKa = 8.53YY119 pKa = 11.24EE120 pKa = 4.2DD121 pKa = 3.77GKK123 pKa = 11.22SYY125 pKa = 10.87HH126 pKa = 6.77GLDD129 pKa = 3.58KK130 pKa = 11.3LSLNNIIQDD139 pKa = 3.55NTYY142 pKa = 9.84MKK144 pKa = 10.54DD145 pKa = 3.59YY146 pKa = 10.88LCYY149 pKa = 10.87QMMGAFGVDD158 pKa = 3.62SPLCSYY164 pKa = 10.78IYY166 pKa = 9.4ITVNGEE172 pKa = 3.56DD173 pKa = 3.09WGLYY177 pKa = 9.44LAVEE181 pKa = 4.5GVEE184 pKa = 4.28EE185 pKa = 4.84SFLQRR190 pKa = 11.84NYY192 pKa = 10.87GSDD195 pKa = 3.4YY196 pKa = 11.49GEE198 pKa = 4.55LYY200 pKa = 10.75KK201 pKa = 10.4PDD203 pKa = 3.78STSMGGGRR211 pKa = 11.84GNGGNFDD218 pKa = 3.57FDD220 pKa = 3.74RR221 pKa = 11.84FQEE224 pKa = 4.2EE225 pKa = 4.35QEE227 pKa = 4.46GQDD230 pKa = 3.41GTTDD234 pKa = 4.18DD235 pKa = 5.59DD236 pKa = 4.12DD237 pKa = 4.31TTGSGSGDD245 pKa = 3.33NTQPTPPDD253 pKa = 4.12GNWGDD258 pKa = 3.69NFSGNMPGPGEE269 pKa = 4.11FPGGSSDD276 pKa = 5.71DD277 pKa = 3.99GMPDD281 pKa = 2.92MGDD284 pKa = 3.07FPGFSEE290 pKa = 5.0DD291 pKa = 3.85DD292 pKa = 3.69SSGEE296 pKa = 4.07TQGNIPAIKK305 pKa = 10.04DD306 pKa = 3.53FTGGSSPEE314 pKa = 4.14GDD316 pKa = 3.95SEE318 pKa = 4.73DD319 pKa = 4.28GPGNMEE325 pKa = 4.4GPGGMGDD332 pKa = 3.5SDD334 pKa = 3.83VSLIYY339 pKa = 10.59TDD341 pKa = 5.42DD342 pKa = 5.38DD343 pKa = 4.19YY344 pKa = 12.13DD345 pKa = 3.81SYY347 pKa = 12.21RR348 pKa = 11.84NIFDD352 pKa = 3.6NAKK355 pKa = 9.47TDD357 pKa = 3.1ITDD360 pKa = 3.29NDD362 pKa = 3.82KK363 pKa = 11.54DD364 pKa = 3.89RR365 pKa = 11.84LIASLKK371 pKa = 10.16QLGDD375 pKa = 3.51HH376 pKa = 6.08QNISDD381 pKa = 3.73IVDD384 pKa = 2.99IDD386 pKa = 3.42EE387 pKa = 4.01VLRR390 pKa = 11.84YY391 pKa = 8.68FVVHH395 pKa = 6.37NFVCNFDD402 pKa = 4.16SYY404 pKa = 9.87TGSMIHH410 pKa = 6.56NYY412 pKa = 9.82YY413 pKa = 10.48LYY415 pKa = 10.83EE416 pKa = 4.34EE417 pKa = 5.32DD418 pKa = 4.64GQLSMIPWDD427 pKa = 3.59YY428 pKa = 11.69NLAFGGFMSMGGATSLVNYY447 pKa = 9.6PIDD450 pKa = 3.82TPVSGGTVDD459 pKa = 4.2SRR461 pKa = 11.84PMLAWIFQNDD471 pKa = 4.36EE472 pKa = 4.05YY473 pKa = 10.95TQMYY477 pKa = 9.52HH478 pKa = 7.8DD479 pKa = 4.97YY480 pKa = 9.56FAQFIADD487 pKa = 3.98TFDD490 pKa = 3.63NGCFASMMDD499 pKa = 3.94KK500 pKa = 10.51VVEE503 pKa = 4.64LISPYY508 pKa = 8.05VQKK511 pKa = 11.01DD512 pKa = 3.23PTKK515 pKa = 8.66FCTYY519 pKa = 10.71EE520 pKa = 3.82EE521 pKa = 4.72FEE523 pKa = 4.45TGISTLEE530 pKa = 3.87QFCTLRR536 pKa = 11.84AQSISGQLDD545 pKa = 3.31GSIPSTSDD553 pKa = 3.02GQSSDD558 pKa = 3.16SSSLIDD564 pKa = 4.48ASQLSIDD571 pKa = 4.0AMGSMGIGGGSGNGHH586 pKa = 5.37GQMNHH591 pKa = 6.04SDD593 pKa = 3.74EE594 pKa = 4.56DD595 pKa = 4.46TNPSNSGDD603 pKa = 3.6IGDD606 pKa = 4.35IPQNNQGDD614 pKa = 4.97FISLLNGTPLSSSSDD629 pKa = 3.21ALFASTSSAFSVSPAAFPAAPDD651 pKa = 3.52STQGNRR657 pKa = 11.84PPSGDD662 pKa = 3.24FDD664 pKa = 4.77GGMGGMGGGPAGSPPGSTDD683 pKa = 3.13DD684 pKa = 4.05SQPSGSTSQSDD695 pKa = 3.53SSSPHH700 pKa = 5.41STNDD704 pKa = 3.3SNDD707 pKa = 3.42NGQSLPSEE715 pKa = 4.46DD716 pKa = 4.12SSPGSGSNSDD726 pKa = 3.46SDD728 pKa = 4.19STQGMQPPSDD738 pKa = 3.63NSGGGMPGQDD748 pKa = 2.99EE749 pKa = 4.31NTPNGMQNPFDD760 pKa = 3.95SQTASSQWGGMDD772 pKa = 3.31TQGWILLGISIFVLGVGLLVACLMKK797 pKa = 10.64GKK799 pKa = 9.69NWW801 pKa = 3.39

Molecular weight: 85.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143ZSJ1|A0A143ZSJ1_9FIRM DNA topoisomerase (ATP-hydrolyzing) OS=Eubacteriaceae bacterium CHKCI005 OX=1780381 GN=gyrB_2 PE=4 SV=1
MM1 pKa = 7.91IYY3 pKa = 9.68TPKK6 pKa = 10.18QRR8 pKa = 11.84GLMMRR13 pKa = 11.84WKK15 pKa = 10.24QNRR18 pKa = 11.84LHH20 pKa = 6.94RR21 pKa = 11.84LNLLEE26 pKa = 4.67GSVSSGKK33 pKa = 7.53TWISLVLWAFWVATMPMDD51 pKa = 3.43KK52 pKa = 10.48LYY54 pKa = 10.71DD55 pKa = 3.51VRR57 pKa = 11.84EE58 pKa = 4.42IPHH61 pKa = 6.67HH62 pKa = 6.32AQAQLPCTVGRR73 pKa = 11.84VSWVIQFCIFSCVQRR88 pKa = 11.84RR89 pKa = 11.84IFVWQEE95 pKa = 3.33SSFGRR100 pKa = 11.84RR101 pKa = 11.84KK102 pKa = 10.04

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2248

0

2248

719107

30

2541

319.9

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.006 ± 0.06

1.536 ± 0.024

5.99 ± 0.047

6.714 ± 0.047

3.82 ± 0.035

7.517 ± 0.048

1.882 ± 0.024

6.388 ± 0.051

5.42 ± 0.048

9.342 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.025

3.718 ± 0.041

4.041 ± 0.039

4.102 ± 0.035

4.965 ± 0.052

6.431 ± 0.049

5.488 ± 0.051

7.249 ± 0.044

0.998 ± 0.023

3.525 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski