Amino acid dipepetide frequency for Synechococcus phage S-CAM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.204AlaAla: 6.204 ± 0.449
0.659AlaCys: 0.659 ± 0.122
4.018AlaAsp: 4.018 ± 0.24
4.323AlaGlu: 4.323 ± 0.406
2.909AlaPhe: 2.909 ± 0.268
6.396AlaGly: 6.396 ± 0.461
0.932AlaHis: 0.932 ± 0.103
3.584AlaIle: 3.584 ± 0.248
3.552AlaLys: 3.552 ± 0.278
4.468AlaLeu: 4.468 ± 0.261
1.446AlaMet: 1.446 ± 0.179
3.664AlaAsn: 3.664 ± 0.259
3.134AlaPro: 3.134 ± 0.242
2.443AlaGln: 2.443 ± 0.242
2.636AlaArg: 2.636 ± 0.212
5.143AlaSer: 5.143 ± 0.322
5.416AlaThr: 5.416 ± 0.389
4.661AlaVal: 4.661 ± 0.342
0.691AlaTrp: 0.691 ± 0.12
2.507AlaTyr: 2.507 ± 0.227
0.0AlaXaa: 0.0 ± 0.0
Cys
0.595CysAla: 0.595 ± 0.112
0.45CysCys: 0.45 ± 0.193
0.627CysAsp: 0.627 ± 0.102
0.948CysGlu: 0.948 ± 0.143
0.354CysPhe: 0.354 ± 0.089
0.755CysGly: 0.755 ± 0.164
0.177CysHis: 0.177 ± 0.047
0.482CysIle: 0.482 ± 0.097
0.53CysLys: 0.53 ± 0.129
0.418CysLeu: 0.418 ± 0.09
0.402CysMet: 0.402 ± 0.09
0.611CysAsn: 0.611 ± 0.128
0.321CysPro: 0.321 ± 0.079
0.289CysGln: 0.289 ± 0.071
0.434CysArg: 0.434 ± 0.108
0.611CysSer: 0.611 ± 0.118
0.45CysThr: 0.45 ± 0.086
0.643CysVal: 0.643 ± 0.093
0.161CysTrp: 0.161 ± 0.076
0.37CysTyr: 0.37 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
4.661AspAla: 4.661 ± 0.329
0.546AspCys: 0.546 ± 0.099
4.886AspAsp: 4.886 ± 0.351
4.243AspGlu: 4.243 ± 0.256
3.391AspPhe: 3.391 ± 0.224
5.738AspGly: 5.738 ± 0.349
1.141AspHis: 1.141 ± 0.148
4.613AspIle: 4.613 ± 0.259
3.568AspLys: 3.568 ± 0.242
5.095AspLeu: 5.095 ± 0.326
1.511AspMet: 1.511 ± 0.173
3.086AspAsn: 3.086 ± 0.24
3.311AspPro: 3.311 ± 0.388
2.041AspGln: 2.041 ± 0.178
2.539AspArg: 2.539 ± 0.195
3.921AspSer: 3.921 ± 0.305
4.307AspThr: 4.307 ± 0.224
4.307AspVal: 4.307 ± 0.232
1.189AspTrp: 1.189 ± 0.17
3.021AspTyr: 3.021 ± 0.253
0.0AspXaa: 0.0 ± 0.0
Glu
3.841GluAla: 3.841 ± 0.313
0.627GluCys: 0.627 ± 0.114
3.921GluAsp: 3.921 ± 0.252
4.693GluGlu: 4.693 ± 0.418
2.845GluPhe: 2.845 ± 0.229
4.323GluGly: 4.323 ± 0.27
1.029GluHis: 1.029 ± 0.14
4.163GluIle: 4.163 ± 0.245
3.729GluLys: 3.729 ± 0.362
4.404GluLeu: 4.404 ± 0.294
1.591GluMet: 1.591 ± 0.191
3.166GluAsn: 3.166 ± 0.228
1.72GluPro: 1.72 ± 0.155
2.604GluGln: 2.604 ± 0.191
2.957GluArg: 2.957 ± 0.239
4.018GluSer: 4.018 ± 0.324
3.905GluThr: 3.905 ± 0.228
4.098GluVal: 4.098 ± 0.281
0.82GluTrp: 0.82 ± 0.125
3.021GluTyr: 3.021 ± 0.253
0.0GluXaa: 0.0 ± 0.0
Phe
2.668PheAla: 2.668 ± 0.252
0.595PheCys: 0.595 ± 0.116
3.568PheAsp: 3.568 ± 0.214
2.732PheGlu: 2.732 ± 0.237
1.832PhePhe: 1.832 ± 0.164
3.198PheGly: 3.198 ± 0.32
0.707PheHis: 0.707 ± 0.114
2.266PheIle: 2.266 ± 0.19
2.33PheLys: 2.33 ± 0.233
3.182PheLeu: 3.182 ± 0.262
0.755PheMet: 0.755 ± 0.122
2.909PheAsn: 2.909 ± 0.252
1.88PhePro: 1.88 ± 0.172
1.495PheGln: 1.495 ± 0.158
1.671PheArg: 1.671 ± 0.159
3.182PheSer: 3.182 ± 0.349
3.311PheThr: 3.311 ± 0.262
3.391PheVal: 3.391 ± 0.302
0.466PheTrp: 0.466 ± 0.101
1.559PheTyr: 1.559 ± 0.177
0.0PheXaa: 0.0 ± 0.0
Gly
5.946GlyAla: 5.946 ± 0.448
0.804GlyCys: 0.804 ± 0.156
5.561GlyAsp: 5.561 ± 0.341
4.339GlyGlu: 4.339 ± 0.279
2.957GlyPhe: 2.957 ± 0.196
8.904GlyGly: 8.904 ± 0.776
1.061GlyHis: 1.061 ± 0.138
4.034GlyIle: 4.034 ± 0.264
3.632GlyLys: 3.632 ± 0.348
4.709GlyLeu: 4.709 ± 0.28
1.688GlyMet: 1.688 ± 0.176
5.095GlyAsn: 5.095 ± 0.415
2.234GlyPro: 2.234 ± 0.201
2.668GlyGln: 2.668 ± 0.204
3.214GlyArg: 3.214 ± 0.25
7.489GlySer: 7.489 ± 0.625
7.409GlyThr: 7.409 ± 0.481
5.513GlyVal: 5.513 ± 0.277
1.157GlyTrp: 1.157 ± 0.15
3.327GlyTyr: 3.327 ± 0.256
0.0GlyXaa: 0.0 ± 0.0
His
0.788HisAla: 0.788 ± 0.114
0.321HisCys: 0.321 ± 0.093
1.045HisAsp: 1.045 ± 0.124
0.916HisGlu: 0.916 ± 0.131
0.82HisPhe: 0.82 ± 0.109
1.077HisGly: 1.077 ± 0.147
0.402HisHis: 0.402 ± 0.091
0.852HisIle: 0.852 ± 0.137
0.964HisLys: 0.964 ± 0.144
1.173HisLeu: 1.173 ± 0.187
0.402HisMet: 0.402 ± 0.087
0.884HisAsn: 0.884 ± 0.129
0.964HisPro: 0.964 ± 0.14
0.627HisGln: 0.627 ± 0.119
0.771HisArg: 0.771 ± 0.106
0.884HisSer: 0.884 ± 0.143
1.141HisThr: 1.141 ± 0.129
0.836HisVal: 0.836 ± 0.127
0.193HisTrp: 0.193 ± 0.051
0.836HisTyr: 0.836 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.773IleAla: 4.773 ± 0.324
0.563IleCys: 0.563 ± 0.097
5.207IleAsp: 5.207 ± 0.281
4.42IleGlu: 4.42 ± 0.284
2.25IlePhe: 2.25 ± 0.2
4.339IleGly: 4.339 ± 0.299
0.723IleHis: 0.723 ± 0.102
3.777IleIle: 3.777 ± 0.311
3.423IleLys: 3.423 ± 0.273
4.13IleLeu: 4.13 ± 0.232
1.109IleMet: 1.109 ± 0.129
3.825IleAsn: 3.825 ± 0.254
2.796IlePro: 2.796 ± 0.185
2.138IleGln: 2.138 ± 0.207
2.684IleArg: 2.684 ± 0.218
4.468IleSer: 4.468 ± 0.221
5.271IleThr: 5.271 ± 0.422
3.938IleVal: 3.938 ± 0.219
0.45IleTrp: 0.45 ± 0.096
1.945IleTyr: 1.945 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
3.504LysAla: 3.504 ± 0.27
0.514LysCys: 0.514 ± 0.092
3.246LysAsp: 3.246 ± 0.233
3.809LysGlu: 3.809 ± 0.374
2.25LysPhe: 2.25 ± 0.19
3.439LysGly: 3.439 ± 0.285
1.093LysHis: 1.093 ± 0.19
3.664LysIle: 3.664 ± 0.233
4.114LysLys: 4.114 ± 0.444
4.709LysLeu: 4.709 ± 0.321
1.623LysMet: 1.623 ± 0.211
2.652LysAsn: 2.652 ± 0.247
2.073LysPro: 2.073 ± 0.203
2.395LysGln: 2.395 ± 0.206
2.202LysArg: 2.202 ± 0.198
3.118LysSer: 3.118 ± 0.274
3.166LysThr: 3.166 ± 0.228
4.163LysVal: 4.163 ± 0.241
0.659LysTrp: 0.659 ± 0.114
2.555LysTyr: 2.555 ± 0.192
0.0LysXaa: 0.0 ± 0.0
Leu
4.307LeuAla: 4.307 ± 0.278
0.627LeuCys: 0.627 ± 0.115
5.175LeuAsp: 5.175 ± 0.278
4.5LeuGlu: 4.5 ± 0.27
3.134LeuPhe: 3.134 ± 0.433
4.918LeuGly: 4.918 ± 0.289
1.366LeuHis: 1.366 ± 0.195
4.323LeuIle: 4.323 ± 0.241
4.773LeuLys: 4.773 ± 0.313
4.902LeuLeu: 4.902 ± 0.355
1.511LeuMet: 1.511 ± 0.185
4.307LeuAsn: 4.307 ± 0.252
3.054LeuPro: 3.054 ± 0.247
2.861LeuGln: 2.861 ± 0.227
3.038LeuArg: 3.038 ± 0.195
4.902LeuSer: 4.902 ± 0.258
5.207LeuThr: 5.207 ± 0.373
4.339LeuVal: 4.339 ± 0.276
0.643LeuTrp: 0.643 ± 0.109
3.038LeuTyr: 3.038 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
1.414MetAla: 1.414 ± 0.164
0.145MetCys: 0.145 ± 0.059
1.238MetAsp: 1.238 ± 0.157
1.205MetGlu: 1.205 ± 0.161
0.739MetPhe: 0.739 ± 0.117
1.205MetGly: 1.205 ± 0.151
0.354MetHis: 0.354 ± 0.086
1.43MetIle: 1.43 ± 0.176
1.607MetLys: 1.607 ± 0.225
1.736MetLeu: 1.736 ± 0.191
0.45MetMet: 0.45 ± 0.087
1.414MetAsn: 1.414 ± 0.184
1.221MetPro: 1.221 ± 0.152
0.82MetGln: 0.82 ± 0.128
0.916MetArg: 0.916 ± 0.142
1.559MetSer: 1.559 ± 0.153
1.623MetThr: 1.623 ± 0.181
1.27MetVal: 1.27 ± 0.156
0.209MetTrp: 0.209 ± 0.069
0.755MetTyr: 0.755 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.713AsnAla: 3.713 ± 0.322
0.434AsnCys: 0.434 ± 0.086
3.134AsnAsp: 3.134 ± 0.219
2.925AsnGlu: 2.925 ± 0.221
2.989AsnPhe: 2.989 ± 0.224
4.645AsnGly: 4.645 ± 0.349
0.916AsnHis: 0.916 ± 0.105
4.082AsnIle: 4.082 ± 0.235
2.829AsnLys: 2.829 ± 0.242
4.082AsnLeu: 4.082 ± 0.255
1.189AsnMet: 1.189 ± 0.135
3.407AsnAsn: 3.407 ± 0.329
2.7AsnPro: 2.7 ± 0.29
2.041AsnGln: 2.041 ± 0.193
2.282AsnArg: 2.282 ± 0.197
3.584AsnSer: 3.584 ± 0.3
4.05AsnThr: 4.05 ± 0.273
4.082AsnVal: 4.082 ± 0.298
0.755AsnTrp: 0.755 ± 0.125
2.443AsnTyr: 2.443 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
2.941ProAla: 2.941 ± 0.222
0.161ProCys: 0.161 ± 0.056
3.166ProAsp: 3.166 ± 0.281
2.893ProGlu: 2.893 ± 0.277
1.736ProPhe: 1.736 ± 0.187
3.841ProGly: 3.841 ± 0.27
0.643ProHis: 0.643 ± 0.106
1.832ProIle: 1.832 ± 0.164
1.913ProLys: 1.913 ± 0.211
2.78ProLeu: 2.78 ± 0.25
0.868ProMet: 0.868 ± 0.145
2.459ProAsn: 2.459 ± 0.235
1.768ProPro: 1.768 ± 0.214
1.43ProGln: 1.43 ± 0.167
1.382ProArg: 1.382 ± 0.161
3.246ProSer: 3.246 ± 0.253
3.166ProThr: 3.166 ± 0.229
3.182ProVal: 3.182 ± 0.253
0.498ProTrp: 0.498 ± 0.087
1.88ProTyr: 1.88 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
2.17GlnAla: 2.17 ± 0.195
0.37GlnCys: 0.37 ± 0.072
1.961GlnAsp: 1.961 ± 0.207
2.62GlnGlu: 2.62 ± 0.245
1.511GlnPhe: 1.511 ± 0.155
2.62GlnGly: 2.62 ± 0.205
0.611GlnHis: 0.611 ± 0.11
2.796GlnIle: 2.796 ± 0.203
2.138GlnLys: 2.138 ± 0.213
2.764GlnLeu: 2.764 ± 0.226
0.739GlnMet: 0.739 ± 0.123
1.784GlnAsn: 1.784 ± 0.181
1.318GlnPro: 1.318 ± 0.151
1.302GlnGln: 1.302 ± 0.153
1.43GlnArg: 1.43 ± 0.133
2.764GlnSer: 2.764 ± 0.19
2.218GlnThr: 2.218 ± 0.188
2.186GlnVal: 2.186 ± 0.167
0.402GlnTrp: 0.402 ± 0.075
2.041GlnTyr: 2.041 ± 0.169
0.0GlnXaa: 0.0 ± 0.0
Arg
2.7ArgAla: 2.7 ± 0.209
0.354ArgCys: 0.354 ± 0.079
2.604ArgAsp: 2.604 ± 0.205
2.411ArgGlu: 2.411 ± 0.247
2.363ArgPhe: 2.363 ± 0.194
3.118ArgGly: 3.118 ± 0.199
0.82ArgHis: 0.82 ± 0.132
2.78ArgIle: 2.78 ± 0.221
2.427ArgLys: 2.427 ± 0.221
3.246ArgLeu: 3.246 ± 0.219
0.932ArgMet: 0.932 ± 0.128
1.913ArgAsn: 1.913 ± 0.156
1.334ArgPro: 1.334 ± 0.151
1.639ArgGln: 1.639 ± 0.167
1.993ArgArg: 1.993 ± 0.187
2.652ArgSer: 2.652 ± 0.16
2.234ArgThr: 2.234 ± 0.277
2.62ArgVal: 2.62 ± 0.269
0.611ArgTrp: 0.611 ± 0.087
2.298ArgTyr: 2.298 ± 0.182
0.0ArgXaa: 0.0 ± 0.0
Ser
4.821SerAla: 4.821 ± 0.341
0.546SerCys: 0.546 ± 0.111
4.371SerAsp: 4.371 ± 0.292
3.439SerGlu: 3.439 ± 0.239
3.214SerPhe: 3.214 ± 0.28
7.489SerGly: 7.489 ± 0.838
0.804SerHis: 0.804 ± 0.108
4.918SerIle: 4.918 ± 0.303
3.166SerLys: 3.166 ± 0.241
5.271SerLeu: 5.271 ± 0.264
1.205SerMet: 1.205 ± 0.147
3.873SerAsn: 3.873 ± 0.271
2.716SerPro: 2.716 ± 0.194
2.411SerGln: 2.411 ± 0.181
2.716SerArg: 2.716 ± 0.229
5.159SerSer: 5.159 ± 0.405
5.368SerThr: 5.368 ± 0.412
4.757SerVal: 4.757 ± 0.25
0.788SerTrp: 0.788 ± 0.125
3.295SerTyr: 3.295 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
5.738ThrAla: 5.738 ± 0.363
0.627ThrCys: 0.627 ± 0.112
4.42ThrAsp: 4.42 ± 0.28
3.584ThrGlu: 3.584 ± 0.259
3.616ThrPhe: 3.616 ± 0.312
6.863ThrGly: 6.863 ± 0.527
1.013ThrHis: 1.013 ± 0.115
5.046ThrIle: 5.046 ± 0.345
3.455ThrLys: 3.455 ± 0.217
5.561ThrLeu: 5.561 ± 0.321
1.157ThrMet: 1.157 ± 0.149
3.713ThrAsn: 3.713 ± 0.312
3.664ThrPro: 3.664 ± 0.221
2.25ThrGln: 2.25 ± 0.209
2.652ThrArg: 2.652 ± 0.246
5.079ThrSer: 5.079 ± 0.339
5.882ThrThr: 5.882 ± 0.488
5.754ThrVal: 5.754 ± 0.32
0.884ThrTrp: 0.884 ± 0.113
2.989ThrTyr: 2.989 ± 0.256
0.0ThrXaa: 0.0 ± 0.0
Val
4.838ValAla: 4.838 ± 0.411
0.546ValCys: 0.546 ± 0.095
4.902ValAsp: 4.902 ± 0.351
4.275ValGlu: 4.275 ± 0.304
2.411ValPhe: 2.411 ± 0.177
5.03ValGly: 5.03 ± 0.268
1.045ValHis: 1.045 ± 0.144
4.082ValIle: 4.082 ± 0.348
3.841ValLys: 3.841 ± 0.278
4.436ValLeu: 4.436 ± 0.342
1.446ValMet: 1.446 ± 0.18
3.841ValAsn: 3.841 ± 0.271
3.327ValPro: 3.327 ± 0.201
2.379ValGln: 2.379 ± 0.226
3.005ValArg: 3.005 ± 0.217
5.127ValSer: 5.127 ± 0.367
5.529ValThr: 5.529 ± 0.362
4.275ValVal: 4.275 ± 0.256
0.627ValTrp: 0.627 ± 0.102
2.957ValTyr: 2.957 ± 0.184
0.0ValXaa: 0.0 ± 0.0
Trp
0.659TrpAla: 0.659 ± 0.098
0.241TrpCys: 0.241 ± 0.083
0.852TrpAsp: 0.852 ± 0.12
0.675TrpGlu: 0.675 ± 0.126
0.434TrpPhe: 0.434 ± 0.096
0.771TrpGly: 0.771 ± 0.103
0.354TrpHis: 0.354 ± 0.076
0.627TrpIle: 0.627 ± 0.116
0.771TrpLys: 0.771 ± 0.113
0.643TrpLeu: 0.643 ± 0.122
0.338TrpMet: 0.338 ± 0.071
0.852TrpAsn: 0.852 ± 0.138
0.209TrpPro: 0.209 ± 0.051
0.53TrpGln: 0.53 ± 0.102
0.691TrpArg: 0.691 ± 0.113
0.755TrpSer: 0.755 ± 0.128
0.868TrpThr: 0.868 ± 0.138
0.788TrpVal: 0.788 ± 0.12
0.161TrpTrp: 0.161 ± 0.054
0.546TrpTyr: 0.546 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.216
0.498TyrCys: 0.498 ± 0.113
3.214TyrAsp: 3.214 ± 0.205
2.523TyrGlu: 2.523 ± 0.213
1.945TyrPhe: 1.945 ± 0.201
3.07TyrGly: 3.07 ± 0.255
0.707TyrHis: 0.707 ± 0.121
2.877TyrIle: 2.877 ± 0.226
2.186TyrLys: 2.186 ± 0.181
3.23TyrLeu: 3.23 ± 0.222
0.932TyrMet: 0.932 ± 0.144
2.829TyrAsn: 2.829 ± 0.214
2.041TyrPro: 2.041 ± 0.188
1.382TyrGln: 1.382 ± 0.155
1.832TyrArg: 1.832 ± 0.185
2.668TyrSer: 2.668 ± 0.211
3.423TyrThr: 3.423 ± 0.242
3.118TyrVal: 3.118 ± 0.227
0.402TyrTrp: 0.402 ± 0.071
2.057TyrTyr: 2.057 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 231 proteins (62223 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski