Tobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain U1))

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus; Tobacco mosaic virus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69687|CAPSD_TMV Capsid protein OS=Tobacco mosaic virus (strain vulgare) OX=12243 GN=CP PE=1 SV=2
MM1 pKa = 7.62SYY3 pKa = 10.89SITTPSQFVFLSSAWADD20 pKa = 3.75PIEE23 pKa = 5.36LINLCTNALGNQFQTQQARR42 pKa = 11.84TVVQRR47 pKa = 11.84QFSEE51 pKa = 3.99VWKK54 pKa = 9.58PSPQVTVRR62 pKa = 11.84FPDD65 pKa = 3.15SDD67 pKa = 3.76FKK69 pKa = 11.2VYY71 pKa = 10.38RR72 pKa = 11.84YY73 pKa = 10.06NAVLDD78 pKa = 4.18PLVTALLGAFDD89 pKa = 3.55TRR91 pKa = 11.84NRR93 pKa = 11.84IIEE96 pKa = 4.22VEE98 pKa = 4.09NQANPTTAEE107 pKa = 4.03TLDD110 pKa = 3.11ATRR113 pKa = 11.84RR114 pKa = 11.84VDD116 pKa = 3.5DD117 pKa = 3.56ATVAIRR123 pKa = 11.84SAINNLIVEE132 pKa = 5.3LIRR135 pKa = 11.84GTGSYY140 pKa = 10.17NRR142 pKa = 11.84SSFEE146 pKa = 4.06SSSGLVWTSGPATT159 pKa = 3.54

Molecular weight: 17.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03586|RDRP_TMV Replicase large subunit OS=Tobacco mosaic virus (strain vulgare) OX=12243 PE=1 SV=2
MM1 pKa = 7.36ALVVKK6 pKa = 10.26GKK8 pKa = 10.77VNINEE13 pKa = 4.75FIDD16 pKa = 3.67LTKK19 pKa = 9.99MEE21 pKa = 5.53KK22 pKa = 9.76ILPSMFTPVKK32 pKa = 10.48SVMCSKK38 pKa = 10.25VDD40 pKa = 3.36KK41 pKa = 11.19IMVHH45 pKa = 5.51EE46 pKa = 4.48NEE48 pKa = 4.63SLSEE52 pKa = 4.11VNLLKK57 pKa = 10.53GVKK60 pKa = 9.92LIDD63 pKa = 3.41SGYY66 pKa = 8.75VCLAGLVVTGEE77 pKa = 3.84WNLPDD82 pKa = 3.53NCRR85 pKa = 11.84GGVSVCLVDD94 pKa = 5.03KK95 pKa = 10.99RR96 pKa = 11.84MEE98 pKa = 4.33RR99 pKa = 11.84ADD101 pKa = 3.63EE102 pKa = 4.26ATLGSYY108 pKa = 7.68YY109 pKa = 10.0TAAAKK114 pKa = 10.21KK115 pKa = 8.92RR116 pKa = 11.84FQFKK120 pKa = 9.97VVPNYY125 pKa = 10.93AITTQDD131 pKa = 2.4AMKK134 pKa = 10.59NVWQVLVNIRR144 pKa = 11.84NVKK147 pKa = 9.22MSAGFCPLSLEE158 pKa = 4.22FVSVCIVYY166 pKa = 10.53RR167 pKa = 11.84NNIKK171 pKa = 10.59LGLRR175 pKa = 11.84EE176 pKa = 4.48KK177 pKa = 9.44ITNVRR182 pKa = 11.84DD183 pKa = 3.72GGPMEE188 pKa = 4.18LTEE191 pKa = 4.11EE192 pKa = 4.32VVDD195 pKa = 4.43EE196 pKa = 4.56FMEE199 pKa = 4.33DD200 pKa = 3.01VPMSIRR206 pKa = 11.84LAKK209 pKa = 10.0FRR211 pKa = 11.84SRR213 pKa = 11.84TGKK216 pKa = 10.45KK217 pKa = 9.53SDD219 pKa = 3.21VRR221 pKa = 11.84KK222 pKa = 10.33GKK224 pKa = 10.15NSSNDD229 pKa = 2.77RR230 pKa = 11.84SVPNKK235 pKa = 10.1NYY237 pKa = 10.64RR238 pKa = 11.84NVKK241 pKa = 10.28DD242 pKa = 3.95FGGMSFKK249 pKa = 10.82KK250 pKa = 10.59NNLIDD255 pKa = 5.08DD256 pKa = 4.44DD257 pKa = 4.9SEE259 pKa = 4.37ATVAEE264 pKa = 4.3SDD266 pKa = 3.89SFF268 pKa = 3.87

Molecular weight: 30.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2043

159

1616

681.0

76.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.755 ± 0.582

1.811 ± 0.248

6.314 ± 0.301

5.874 ± 0.308

4.748 ± 0.189

4.503 ± 0.428

2.056 ± 0.897

4.993 ± 0.168

6.559 ± 1.19

9.3 ± 0.884

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.541

4.405 ± 1.282

3.377 ± 0.351

3.279 ± 0.664

5.384 ± 0.344

8.37 ± 0.355

5.776 ± 0.9

8.762 ± 1.05

0.979 ± 0.192

3.769 ± 0.697
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski