Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) (Campylobacter mustelae)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter mustelae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3UI31|D3UI31_HELM1 Putative integral membrane protein OS=Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) OX=679897 GN=HMU08970 PE=4 SV=1
MM1 pKa = 7.58CLAIPSKK8 pKa = 10.73VISIDD13 pKa = 3.17EE14 pKa = 4.33STNTATIQTLGVQRR28 pKa = 11.84NASLDD33 pKa = 3.63LMQDD37 pKa = 3.16PVEE40 pKa = 5.13IGDD43 pKa = 3.86YY44 pKa = 10.3VLLHH48 pKa = 6.57IGYY51 pKa = 9.87VMSKK55 pKa = 9.52IDD57 pKa = 4.05EE58 pKa = 4.23EE59 pKa = 4.52DD60 pKa = 3.31ALEE63 pKa = 4.07SLKK66 pKa = 10.89LYY68 pKa = 10.57EE69 pKa = 4.96QMIQNMEE76 pKa = 4.07EE77 pKa = 3.91EE78 pKa = 4.62EE79 pKa = 4.49EE80 pKa = 4.49YY81 pKa = 10.43IDD83 pKa = 5.59PIYY86 pKa = 11.03KK87 pKa = 10.09EE88 pKa = 4.0

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3UHN2|D3UHN2_HELM1 Polyribonucleotide nucleotidyltransferase OS=Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) OX=679897 GN=pnp PE=3 SV=1
MM1 pKa = 7.41NGFAFFSSLPQACFLRR17 pKa = 11.84GFFPNPLCGRR27 pKa = 11.84YY28 pKa = 8.51FFPLSPEE35 pKa = 3.79RR36 pKa = 11.84FLFSAYY42 pKa = 9.92HH43 pKa = 5.37VGCFFPSMTRR53 pKa = 11.84GAFSIPCGEE62 pKa = 4.12SLFFFDD68 pKa = 5.19KK69 pKa = 11.58GEE71 pKa = 3.93GLKK74 pKa = 10.1YY75 pKa = 10.51AKK77 pKa = 10.04SLPPPYY83 pKa = 9.95KK84 pKa = 10.26SPTPTRR90 pKa = 11.84STFYY94 pKa = 10.4RR95 pKa = 11.84GQLPTEE101 pKa = 4.33IFQILFSCALLALGFSPASWCKK123 pKa = 10.11KK124 pKa = 9.85RR125 pKa = 11.84EE126 pKa = 4.0LRR128 pKa = 11.84KK129 pKa = 9.83QKK131 pKa = 10.26CFHH134 pKa = 6.84PFFARR139 pKa = 11.84SGKK142 pKa = 10.14ARR144 pKa = 11.84LSSSLPKK151 pKa = 9.98KK152 pKa = 9.68RR153 pKa = 11.84RR154 pKa = 11.84HH155 pKa = 5.54FLGSAARR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84CTEE167 pKa = 3.61RR168 pKa = 11.84GGVKK172 pKa = 10.46AFFARR177 pKa = 11.84KK178 pKa = 9.37ASWAYY183 pKa = 10.26RR184 pKa = 11.84EE185 pKa = 4.29KK186 pKa = 10.89KK187 pKa = 10.29KK188 pKa = 10.91NPLKK192 pKa = 10.7LFEE195 pKa = 4.5EE196 pKa = 4.69RR197 pKa = 11.84KK198 pKa = 6.93PTQRR202 pKa = 11.84GWRR205 pKa = 11.84KK206 pKa = 8.46RR207 pKa = 11.84CLSHH211 pKa = 7.44RR212 pKa = 11.84EE213 pKa = 3.73SHH215 pKa = 6.94LPP217 pKa = 3.42

Molecular weight: 25.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1400

0

1400

463791

31

4094

331.3

37.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.177 ± 0.068

1.188 ± 0.03

4.874 ± 0.047

6.32 ± 0.086

5.43 ± 0.067

6.863 ± 0.103

2.207 ± 0.039

8.153 ± 0.062

7.615 ± 0.079

10.397 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.033

4.939 ± 0.121

3.523 ± 0.037

4.183 ± 0.048

3.984 ± 0.045

6.589 ± 0.063

4.492 ± 0.094

5.464 ± 0.057

0.725 ± 0.019

3.541 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski