Amino acid dipepetide frequency for Clostridium pasteurianum BC1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.673AlaAla: 4.673 ± 0.08	0.723AlaCys: 0.723 ± 0.025	2.889AlaAsp: 2.889 ± 0.057	3.55AlaGlu: 3.55 ± 0.057	2.663AlaPhe: 2.663 ± 0.054	3.937AlaGly: 3.937 ± 0.069	0.85AlaHis: 0.85 ± 0.023	5.712AlaIle: 5.712 ± 0.072	4.681AlaLys: 4.681 ± 0.071	5.872AlaLeu: 5.872 ± 0.09	1.644AlaMet: 1.644 ± 0.037	2.719AlaAsn: 2.719 ± 0.051	1.481AlaPro: 1.481 ± 0.044	1.545AlaGln: 1.545 ± 0.039	1.863AlaArg: 1.863 ± 0.041	3.568AlaSer: 3.568 ± 0.06	2.668AlaThr: 2.668 ± 0.056	4.496AlaVal: 4.496 ± 0.062	0.426AlaTrp: 0.426 ± 0.019	2.17AlaTyr: 2.17 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.651CysAla: 0.651 ± 0.021	0.212CysCys: 0.212 ± 0.014	0.611CysAsp: 0.611 ± 0.023	0.75CysGlu: 0.75 ± 0.025	0.462CysPhe: 0.462 ± 0.021	1.14CysGly: 1.14 ± 0.031	0.222CysHis: 0.222 ± 0.013	1.137CysIle: 1.137 ± 0.035	0.993CysLys: 0.993 ± 0.031	0.884CysLeu: 0.884 ± 0.025	0.277CysMet: 0.277 ± 0.017	0.715CysAsn: 0.715 ± 0.025	0.49CysPro: 0.49 ± 0.023	0.195CysGln: 0.195 ± 0.012	0.399CysArg: 0.399 ± 0.018	0.865CysSer: 0.865 ± 0.027	0.659CysThr: 0.659 ± 0.02	0.64CysVal: 0.64 ± 0.025	0.084CysTrp: 0.084 ± 0.009	0.439CysTyr: 0.439 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	2.801AspAla: 2.801 ± 0.054	0.58AspCys: 0.58 ± 0.024	2.6AspAsp: 2.6 ± 0.058	4.056AspGlu: 4.056 ± 0.065	2.691AspPhe: 2.691 ± 0.051	3.252AspGly: 3.252 ± 0.062	0.603AspHis: 0.603 ± 0.023	6.351AspIle: 6.351 ± 0.072	5.461AspLys: 5.461 ± 0.072	4.684AspLeu: 4.684 ± 0.061	1.446AspMet: 1.446 ± 0.037	3.608AspAsn: 3.608 ± 0.057	1.443AspPro: 1.443 ± 0.036	0.913AspGln: 0.913 ± 0.029	1.869AspArg: 1.869 ± 0.045	3.254AspSer: 3.254 ± 0.054	2.744AspThr: 2.744 ± 0.051	3.46AspVal: 3.46 ± 0.06	0.403AspTrp: 0.403 ± 0.019	2.546AspTyr: 2.546 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	4.122GluAla: 4.122 ± 0.07	0.616GluCys: 0.616 ± 0.025	3.982GluAsp: 3.982 ± 0.068	5.841GluGlu: 5.841 ± 0.079	2.831GluPhe: 2.831 ± 0.049	3.643GluGly: 3.643 ± 0.055	0.936GluHis: 0.936 ± 0.028	6.699GluIle: 6.699 ± 0.084	7.254GluLys: 7.254 ± 0.09	6.384GluLeu: 6.384 ± 0.077	1.69GluMet: 1.69 ± 0.033	5.124GluAsn: 5.124 ± 0.068	1.389GluPro: 1.389 ± 0.032	1.768GluGln: 1.768 ± 0.038	2.53GluArg: 2.53 ± 0.049	3.207GluSer: 3.207 ± 0.054	2.802GluThr: 2.802 ± 0.045	4.134GluVal: 4.134 ± 0.064	0.473GluTrp: 0.473 ± 0.021	2.702GluTyr: 2.702 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.33PheAla: 2.33 ± 0.05	0.55PheCys: 0.55 ± 0.02	2.429PheAsp: 2.429 ± 0.052	2.502PheGlu: 2.502 ± 0.05	2.104PhePhe: 2.104 ± 0.05	2.926PheGly: 2.926 ± 0.057	0.648PheHis: 0.648 ± 0.022	4.767PheIle: 4.767 ± 0.075	3.789PheLys: 3.789 ± 0.056	4.061PheLeu: 4.061 ± 0.066	1.134PheMet: 1.134 ± 0.032	3.041PheAsn: 3.041 ± 0.052	1.332PhePro: 1.332 ± 0.032	1.176PheGln: 1.176 ± 0.029	1.345PheArg: 1.345 ± 0.033	3.296PheSer: 3.296 ± 0.053	2.396PheThr: 2.396 ± 0.048	2.638PheVal: 2.638 ± 0.054	0.343PheTrp: 0.343 ± 0.019	1.797PheTyr: 1.797 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	3.969GlyAla: 3.969 ± 0.065	0.953GlyCys: 0.953 ± 0.033	3.083GlyAsp: 3.083 ± 0.05	3.889GlyGlu: 3.889 ± 0.06	3.023GlyPhe: 3.023 ± 0.059	4.275GlyGly: 4.275 ± 0.072	1.028GlyHis: 1.028 ± 0.026	7.039GlyIle: 7.039 ± 0.082	5.55GlyLys: 5.55 ± 0.069	5.31GlyLeu: 5.31 ± 0.07	1.729GlyMet: 1.729 ± 0.042	3.494GlyAsn: 3.494 ± 0.065	1.262GlyPro: 1.262 ± 0.036	1.549GlyGln: 1.549 ± 0.034	2.221GlyArg: 2.221 ± 0.042	3.841GlySer: 3.841 ± 0.061	3.546GlyThr: 3.546 ± 0.06	4.368GlyVal: 4.368 ± 0.069	0.538GlyTrp: 0.538 ± 0.022	2.958GlyTyr: 2.958 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.694HisAla: 0.694 ± 0.022	0.232HisCys: 0.232 ± 0.013	0.725HisAsp: 0.725 ± 0.023	0.907HisGlu: 0.907 ± 0.027	0.67HisPhe: 0.67 ± 0.023	1.03HisGly: 1.03 ± 0.03	0.286HisHis: 0.286 ± 0.017	1.421HisIle: 1.421 ± 0.035	1.148HisLys: 1.148 ± 0.028	1.144HisLeu: 1.144 ± 0.031	0.401HisMet: 0.401 ± 0.02	0.89HisAsn: 0.89 ± 0.026	0.613HisPro: 0.613 ± 0.023	0.322HisGln: 0.322 ± 0.017	0.593HisArg: 0.593 ± 0.02	0.975HisSer: 0.975 ± 0.029	0.747HisThr: 0.747 ± 0.023	0.788HisVal: 0.788 ± 0.024	0.143HisTrp: 0.143 ± 0.01	0.596HisTyr: 0.596 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.01IleAla: 6.01 ± 0.072	1.273IleCys: 1.273 ± 0.036	5.942IleAsp: 5.942 ± 0.073	6.738IleGlu: 6.738 ± 0.075	4.496IlePhe: 4.496 ± 0.072	6.272IleGly: 6.272 ± 0.085	1.353IleHis: 1.353 ± 0.03	9.904IleIle: 9.904 ± 0.121	8.894IleLys: 8.894 ± 0.087	9.103IleLeu: 9.103 ± 0.111	2.366IleMet: 2.366 ± 0.046	6.616IleAsn: 6.616 ± 0.081	3.419IlePro: 3.419 ± 0.047	2.324IleGln: 2.324 ± 0.05	3.151IleArg: 3.151 ± 0.054	7.044IleSer: 7.044 ± 0.094	5.002IleThr: 5.002 ± 0.067	6.307IleVal: 6.307 ± 0.073	0.676IleTrp: 0.676 ± 0.027	3.786IleTyr: 3.786 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.2LysAla: 5.2 ± 0.068	0.93LysCys: 0.93 ± 0.026	5.863LysAsp: 5.863 ± 0.073	7.369LysGlu: 7.369 ± 0.092	3.411LysPhe: 3.411 ± 0.055	4.892LysGly: 4.892 ± 0.059	1.131LysHis: 1.131 ± 0.031	8.551LysIle: 8.551 ± 0.086	8.045LysLys: 8.045 ± 0.101	7.953LysLeu: 7.953 ± 0.073	2.242LysMet: 2.242 ± 0.041	6.767LysAsn: 6.767 ± 0.09	2.192LysPro: 2.192 ± 0.05	2.428LysGln: 2.428 ± 0.044	3.025LysArg: 3.025 ± 0.048	5.175LysSer: 5.175 ± 0.061	4.067LysThr: 4.067 ± 0.058	5.504LysVal: 5.504 ± 0.07	0.688LysTrp: 0.688 ± 0.026	4.107LysTyr: 4.107 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	5.094LeuAla: 5.094 ± 0.068	1.081LeuCys: 1.081 ± 0.029	4.949LeuAsp: 4.949 ± 0.061	5.691LeuGlu: 5.691 ± 0.09	3.746LeuPhe: 3.746 ± 0.065	5.833LeuGly: 5.833 ± 0.075	1.257LeuHis: 1.257 ± 0.037	8.489LeuIle: 8.489 ± 0.114	8.636LeuLys: 8.636 ± 0.087	7.8LeuLeu: 7.8 ± 0.094	2.252LeuMet: 2.252 ± 0.041	6.016LeuAsn: 6.016 ± 0.071	2.872LeuPro: 2.872 ± 0.048	2.462LeuGln: 2.462 ± 0.046	3.163LeuArg: 3.163 ± 0.051	6.58LeuSer: 6.58 ± 0.076	4.438LeuThr: 4.438 ± 0.066	5.069LeuVal: 5.069 ± 0.064	0.636LeuTrp: 0.636 ± 0.023	3.236LeuTyr: 3.236 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.619MetAla: 1.619 ± 0.042	0.257MetCys: 0.257 ± 0.017	1.573MetAsp: 1.573 ± 0.035	1.772MetGlu: 1.772 ± 0.037	0.926MetPhe: 0.926 ± 0.028	1.725MetGly: 1.725 ± 0.039	0.367MetHis: 0.367 ± 0.019	2.106MetIle: 2.106 ± 0.042	2.414MetLys: 2.414 ± 0.044	2.311MetLeu: 2.311 ± 0.041	0.632MetMet: 0.632 ± 0.032	1.687MetAsn: 1.687 ± 0.038	0.894MetPro: 0.894 ± 0.027	0.649MetGln: 0.649 ± 0.023	0.866MetArg: 0.866 ± 0.026	1.744MetSer: 1.744 ± 0.035	1.166MetThr: 1.166 ± 0.028	1.417MetVal: 1.417 ± 0.037	0.169MetTrp: 0.169 ± 0.011	0.853MetTyr: 0.853 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.151AsnAla: 3.151 ± 0.06	0.763AsnCys: 0.763 ± 0.024	2.994AsnAsp: 2.994 ± 0.048	4.025AsnGlu: 4.025 ± 0.064	2.928AsnPhe: 2.928 ± 0.05	3.874AsnGly: 3.874 ± 0.067	0.82AsnHis: 0.82 ± 0.026	7.468AsnIle: 7.468 ± 0.084	6.081AsnLys: 6.081 ± 0.075	5.642AsnLeu: 5.642 ± 0.071	1.739AsnMet: 1.739 ± 0.037	4.937AsnAsn: 4.937 ± 0.089	2.256AsnPro: 2.256 ± 0.043	1.438AsnGln: 1.438 ± 0.034	2.173AsnArg: 2.173 ± 0.038	4.44AsnSer: 4.44 ± 0.079	3.406AsnThr: 3.406 ± 0.06	3.776AsnVal: 3.776 ± 0.06	0.519AsnTrp: 0.519 ± 0.026	2.886AsnTyr: 2.886 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	1.558ProAla: 1.558 ± 0.042	0.34ProCys: 0.34 ± 0.016	1.622ProAsp: 1.622 ± 0.032	2.246ProGlu: 2.246 ± 0.048	1.438ProPhe: 1.438 ± 0.039	1.811ProGly: 1.811 ± 0.045	0.483ProHis: 0.483 ± 0.02	2.758ProIle: 2.758 ± 0.041	2.38ProLys: 2.38 ± 0.045	2.562ProLeu: 2.562 ± 0.05	0.734ProMet: 0.734 ± 0.025	1.608ProAsn: 1.608 ± 0.038	0.706ProPro: 0.706 ± 0.022	0.855ProGln: 0.855 ± 0.027	0.895ProArg: 0.895 ± 0.028	1.911ProSer: 1.911 ± 0.036	1.606ProThr: 1.606 ± 0.038	2.222ProVal: 2.222 ± 0.046	0.268ProTrp: 0.268 ± 0.016	1.286ProTyr: 1.286 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	1.473GlnAla: 1.473 ± 0.04	0.329GlnCys: 0.329 ± 0.017	1.343GlnAsp: 1.343 ± 0.036	1.637GlnGlu: 1.637 ± 0.036	1.113GlnPhe: 1.113 ± 0.032	1.636GlnGly: 1.636 ± 0.037	0.425GlnHis: 0.425 ± 0.02	2.333GlnIle: 2.333 ± 0.048	2.048GlnLys: 2.048 ± 0.049	2.402GlnLeu: 2.402 ± 0.047	0.635GlnMet: 0.635 ± 0.021	1.623GlnAsn: 1.623 ± 0.04	0.684GlnPro: 0.684 ± 0.027	0.985GlnGln: 0.985 ± 0.039	0.986GlnArg: 0.986 ± 0.024	1.499GlnSer: 1.499 ± 0.041	1.175GlnThr: 1.175 ± 0.035	1.564GlnVal: 1.564 ± 0.039	0.285GlnTrp: 0.285 ± 0.017	1.184GlnTyr: 1.184 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	1.798ArgAla: 1.798 ± 0.038	0.353ArgCys: 0.353 ± 0.018	1.881ArgAsp: 1.881 ± 0.04	2.904ArgGlu: 2.904 ± 0.06	1.453ArgPhe: 1.453 ± 0.036	2.005ArgGly: 2.005 ± 0.042	0.54ArgHis: 0.54 ± 0.019	3.251ArgIle: 3.251 ± 0.051	3.101ArgLys: 3.101 ± 0.048	3.01ArgLeu: 3.01 ± 0.061	0.833ArgMet: 0.833 ± 0.025	2.205ArgAsn: 2.205 ± 0.041	0.928ArgPro: 0.928 ± 0.031	1.017ArgGln: 1.017 ± 0.028	1.446ArgArg: 1.446 ± 0.045	1.709ArgSer: 1.709 ± 0.035	1.633ArgThr: 1.633 ± 0.036	2.133ArgVal: 2.133 ± 0.046	0.278ArgTrp: 0.278 ± 0.017	1.377ArgTyr: 1.377 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.417SerAla: 3.417 ± 0.054	0.68SerCys: 0.68 ± 0.025	3.204SerAsp: 3.204 ± 0.058	3.982SerGlu: 3.982 ± 0.061	3.123SerPhe: 3.123 ± 0.06	4.488SerGly: 4.488 ± 0.066	0.95SerHis: 0.95 ± 0.027	6.856SerIle: 6.856 ± 0.082	5.745SerLys: 5.745 ± 0.079	5.747SerLeu: 5.747 ± 0.076	1.605SerMet: 1.605 ± 0.037	4.237SerAsn: 4.237 ± 0.082	1.831SerPro: 1.831 ± 0.04	1.768SerGln: 1.768 ± 0.042	2.095SerArg: 2.095 ± 0.045	4.607SerSer: 4.607 ± 0.084	3.36SerThr: 3.36 ± 0.062	3.92SerVal: 3.92 ± 0.049	0.507SerTrp: 0.507 ± 0.022	2.495SerTyr: 2.495 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	3.369ThrAla: 3.369 ± 0.064	0.53ThrCys: 0.53 ± 0.02	2.502ThrAsp: 2.502 ± 0.051	3.061ThrGlu: 3.061 ± 0.051	2.248ThrPhe: 2.248 ± 0.043	3.737ThrGly: 3.737 ± 0.057	0.752ThrHis: 0.752 ± 0.023	4.854ThrIle: 4.854 ± 0.069	3.726ThrLys: 3.726 ± 0.064	4.642ThrLeu: 4.642 ± 0.064	1.128ThrMet: 1.128 ± 0.03	2.715ThrAsn: 2.715 ± 0.05	1.885ThrPro: 1.885 ± 0.043	1.23ThrGln: 1.23 ± 0.035	1.486ThrArg: 1.486 ± 0.031	3.326ThrSer: 3.326 ± 0.061	2.815ThrThr: 2.815 ± 0.065	3.555ThrVal: 3.555 ± 0.057	0.399ThrTrp: 0.399 ± 0.019	1.945ThrTyr: 1.945 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	3.738ValAla: 3.738 ± 0.059	0.761ValCys: 0.761 ± 0.028	3.694ValAsp: 3.694 ± 0.064	4.145ValGlu: 4.145 ± 0.065	2.893ValPhe: 2.893 ± 0.047	4.043ValGly: 4.043 ± 0.065	0.901ValHis: 0.901 ± 0.028	6.223ValIle: 6.223 ± 0.069	5.37ValLys: 5.37 ± 0.067	5.614ValLeu: 5.614 ± 0.067	1.46ValMet: 1.46 ± 0.032	3.868ValAsn: 3.868 ± 0.055	2.128ValPro: 2.128 ± 0.041	1.626ValGln: 1.626 ± 0.042	1.945ValArg: 1.945 ± 0.041	4.244ValSer: 4.244 ± 0.065	3.331ValThr: 3.331 ± 0.056	4.273ValVal: 4.273 ± 0.064	0.43ValTrp: 0.43 ± 0.018	2.369ValTyr: 2.369 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.433TrpAla: 0.433 ± 0.021	0.103TrpCys: 0.103 ± 0.009	0.435TrpAsp: 0.435 ± 0.017	0.436TrpGlu: 0.436 ± 0.02	0.363TrpPhe: 0.363 ± 0.017	0.52TrpGly: 0.52 ± 0.021	0.156TrpHis: 0.156 ± 0.012	0.737TrpIle: 0.737 ± 0.025	0.659TrpLys: 0.659 ± 0.025	0.656TrpLeu: 0.656 ± 0.022	0.2TrpMet: 0.2 ± 0.012	0.527TrpAsn: 0.527 ± 0.023	0.183TrpPro: 0.183 ± 0.012	0.271TrpGln: 0.271 ± 0.017	0.29TrpArg: 0.29 ± 0.016	0.497TrpSer: 0.497 ± 0.024	0.355TrpThr: 0.355 ± 0.018	0.446TrpVal: 0.446 ± 0.019	0.106TrpTrp: 0.106 ± 0.009	0.303TrpTyr: 0.303 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.032TyrAla: 2.032 ± 0.044	0.557TyrCys: 0.557 ± 0.023	2.444TyrAsp: 2.444 ± 0.049	2.627TyrGlu: 2.627 ± 0.051	2.022TyrPhe: 2.022 ± 0.047	2.585TyrGly: 2.585 ± 0.046	0.616TyrHis: 0.616 ± 0.022	4.017TyrIle: 4.017 ± 0.058	3.566TyrLys: 3.566 ± 0.053	3.519TyrLeu: 3.519 ± 0.058	1.003TyrMet: 1.003 ± 0.03	2.92TyrAsn: 2.92 ± 0.052	1.306TyrPro: 1.306 ± 0.031	0.838TyrGln: 0.838 ± 0.026	1.513TyrArg: 1.513 ± 0.034	2.831TyrSer: 2.831 ± 0.05	1.99TyrThr: 1.99 ± 0.04	2.344TyrVal: 2.344 ± 0.037	0.303TyrTrp: 0.303 ± 0.017	1.835TyrTyr: 1.835 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4454 proteins (1282591 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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