Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; Deinococcus radiodurans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9RZT0|Q9RZT0_DEIRA Arylesterase/monoxygenase OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=DR_B0033 PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 5.29LSEE5 pKa = 4.12LAHH8 pKa = 6.9RR9 pKa = 11.84ITYY12 pKa = 9.63RR13 pKa = 11.84AYY15 pKa = 10.05EE16 pKa = 4.16LDD18 pKa = 4.33GDD20 pKa = 4.81DD21 pKa = 5.1LDD23 pKa = 5.42SLAGLCGLMSWHH35 pKa = 6.33TLIAPLTFQEE45 pKa = 5.14FGTEE49 pKa = 3.9DD50 pKa = 3.93GRR52 pKa = 11.84TLLCAADD59 pKa = 4.85EE60 pKa = 4.47SGLWITLTDD69 pKa = 3.98GAAGVPTSPDD79 pKa = 3.11TFQLSLAEE87 pKa = 4.81DD88 pKa = 4.08LLSEE92 pKa = 4.17PVYY95 pKa = 10.53TLDD98 pKa = 3.25VVNGHH103 pKa = 5.84VVQTAPGLNN112 pKa = 3.47

Molecular weight:
12.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9RWZ6|Q9RWZ6_DEIRA Uncharacterized protein OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=DR_0519 PE=4 SV=1
MM1 pKa = 7.58LCGGTRR7 pKa = 11.84GVVSKK12 pKa = 11.03AVGPFPGQGGEE23 pKa = 4.01QARR26 pKa = 11.84QRR28 pKa = 11.84GPQRR32 pKa = 11.84QGGSEE37 pKa = 4.33MVWEE41 pKa = 4.49TLPEE45 pKa = 4.18AASLFACSSPGAKK58 pKa = 9.73AGRR61 pKa = 11.84TARR64 pKa = 11.84QHH66 pKa = 4.98STLARR71 pKa = 11.84ALAAASPSDD80 pKa = 3.53RR81 pKa = 11.84TCTVLSQKK89 pKa = 10.49AQARR93 pKa = 11.84PAGQNFRR100 pKa = 11.84WGPTQGNVKK109 pKa = 9.35PRR111 pKa = 11.84KK112 pKa = 8.39RR113 pKa = 11.84NVRR116 pKa = 11.84NFRR119 pKa = 11.84LLYY122 pKa = 8.44GQAGAGIPVTANAISRR138 pKa = 11.84QRR140 pKa = 11.84GTIHH144 pKa = 6.49TLSAAPNHH152 pKa = 6.55RR153 pKa = 11.84RR154 pKa = 11.84FTMKK158 pKa = 9.45NTTQGFTLIEE168 pKa = 4.17LLIVIAIIGILAAVLIPNLLGARR191 pKa = 11.84AKK193 pKa = 10.99ANDD196 pKa = 3.96SATQSYY202 pKa = 9.98IRR204 pKa = 11.84NCYY207 pKa = 8.03TAVEE211 pKa = 4.5VARR214 pKa = 11.84GADD217 pKa = 3.33GRR219 pKa = 11.84LPAAGNCDD227 pKa = 3.02STANLGTNAVATPGAITAAGAYY249 pKa = 9.94SLDD252 pKa = 3.44GTGSKK257 pKa = 10.3FAVTATSVTNKK268 pKa = 8.3TFCFDD273 pKa = 3.43GAKK276 pKa = 9.61MGEE279 pKa = 4.29GTGATGACTFSTSTGTGTGTGTGTGTGTGTGTGSNN314 pKa = 3.8

Molecular weight:
31.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3085

0

3085

949308

37

1940

307.7

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.205 ± 0.064

0.668 ± 0.013

5.069 ± 0.035

5.731 ± 0.046

3.156 ± 0.027

9.198 ± 0.053

2.085 ± 0.025

3.284 ± 0.037

2.717 ± 0.041

11.65 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.891 ± 0.023

2.407 ± 0.027

6.049 ± 0.041

4.118 ± 0.035

7.375 ± 0.042

5.2 ± 0.031

5.815 ± 0.041

7.687 ± 0.035

1.388 ± 0.017

2.303 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski