Trypanosoma brucei brucei (strain 927/4 GUTat10.1)

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Trypanozoon; Trypanosoma brucei; Trypanosoma brucei brucei

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8587 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38EE3|Q38EE3_TRYB2 Pescadillo homolog OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb09.211.0180 PE=3 SV=1
MM1 pKa = 7.75SDD3 pKa = 4.24DD4 pKa = 4.65DD5 pKa = 5.4DD6 pKa = 5.32DD7 pKa = 4.98VVVLQVCANKK17 pKa = 9.86QCLGIEE23 pKa = 4.14DD24 pKa = 4.34LQFDD28 pKa = 3.89EE29 pKa = 6.16AMGEE33 pKa = 4.3MYY35 pKa = 10.42CANCRR40 pKa = 11.84EE41 pKa = 4.41LYY43 pKa = 10.55NRR45 pKa = 11.84AAQEE49 pKa = 3.94GFRR52 pKa = 11.84ILLSPEE58 pKa = 3.94DD59 pKa = 3.81VVLVRR64 pKa = 11.84MIFDD68 pKa = 3.71RR69 pKa = 11.84FDD71 pKa = 3.1NGKK74 pKa = 10.7GYY76 pKa = 7.36WTHH79 pKa = 7.25DD80 pKa = 3.24DD81 pKa = 3.57FVRR84 pKa = 11.84FQEE87 pKa = 4.21STLQDD92 pKa = 3.32VEE94 pKa = 4.12TDD96 pKa = 3.72INSHH100 pKa = 5.45EE101 pKa = 4.02ALKK104 pKa = 10.89EE105 pKa = 4.0FFKK108 pKa = 11.08DD109 pKa = 3.4EE110 pKa = 4.09YY111 pKa = 10.97DD112 pKa = 3.64IEE114 pKa = 4.42LTPSSSGEE122 pKa = 3.88YY123 pKa = 10.44NILPEE128 pKa = 4.52NLEE131 pKa = 4.02EE132 pKa = 4.29MYY134 pKa = 10.86GGYY137 pKa = 10.67AYY139 pKa = 11.34NNINALHH146 pKa = 6.92KK147 pKa = 11.03DD148 pKa = 3.3CDD150 pKa = 3.88ALEE153 pKa = 4.57SIGLINTAVLEE164 pKa = 4.25

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q584Q9|Q584Q9_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb927.6.2470 PE=4 SV=1
MM1 pKa = 7.39NLPFPTHH8 pKa = 6.09NKK10 pKa = 8.63EE11 pKa = 3.91ALPFNEE17 pKa = 4.48DD18 pKa = 3.03AGPEE22 pKa = 4.33SRR24 pKa = 11.84SHH26 pKa = 6.79PSSDD30 pKa = 2.77SVRR33 pKa = 11.84IYY35 pKa = 10.81FSLGEE40 pKa = 3.92MAAAVSPLHH49 pKa = 6.61RR50 pKa = 11.84CFSFPVTRR58 pKa = 11.84PFVSPCIRR66 pKa = 11.84CPNCKK71 pKa = 9.81GCQLHH76 pKa = 5.82MQFCSGAYY84 pKa = 9.71GLRR87 pKa = 11.84HH88 pKa = 4.7TLAWIVRR95 pKa = 11.84RR96 pKa = 11.84HH97 pKa = 5.58PPAAEE102 pKa = 3.73EE103 pKa = 3.84RR104 pKa = 11.84RR105 pKa = 11.84TIKK108 pKa = 10.44LRR110 pKa = 11.84SQPPSAGGGAACRR123 pKa = 11.84MVRR126 pKa = 11.84RR127 pKa = 11.84VCRR130 pKa = 11.84FEE132 pKa = 3.89VAPVVITQHH141 pKa = 4.86VTHH144 pKa = 6.67VDD146 pKa = 3.33YY147 pKa = 11.48NQTIDD152 pKa = 3.44TARR155 pKa = 11.84HH156 pKa = 5.19GGSRR160 pKa = 11.84GPFAGMVCPRR170 pKa = 11.84PQEE173 pKa = 3.71IRR175 pKa = 11.84SVYY178 pKa = 10.08SRR180 pKa = 11.84IFHH183 pKa = 4.84EE184 pKa = 4.99HH185 pKa = 5.63IRR187 pKa = 11.84TGGSRR192 pKa = 11.84LPFRR196 pKa = 11.84HH197 pKa = 5.55FQRR200 pKa = 11.84TAMRR204 pKa = 11.84SLAGEE209 pKa = 4.18AQALWEE215 pKa = 4.48KK216 pKa = 10.17LTFAPEE222 pKa = 4.42GSRR225 pKa = 11.84LDD227 pKa = 3.91EE228 pKa = 4.28PPMRR232 pKa = 11.84TEE234 pKa = 4.72RR235 pKa = 11.84GDD237 pKa = 3.42KK238 pKa = 10.08VPRR241 pKa = 11.84RR242 pKa = 11.84VPIGWKK248 pKa = 9.78CPMFFVSLLPRR259 pKa = 11.84AAAGNNGITFKK270 pKa = 10.9LWLPEE275 pKa = 3.88ALVHH279 pKa = 7.4DD280 pKa = 5.39FLTAHH285 pKa = 5.96IVEE288 pKa = 4.35VEE290 pKa = 4.19RR291 pKa = 11.84VRR293 pKa = 11.84EE294 pKa = 4.02LPHH297 pKa = 5.37TCRR300 pKa = 11.84FRR302 pKa = 11.84CLSTDD307 pKa = 2.85TMLEE311 pKa = 3.93VEE313 pKa = 4.62DD314 pKa = 5.84AIASVNALLPRR325 pKa = 11.84WEE327 pKa = 5.02GEE329 pKa = 4.3LKK331 pKa = 10.66CPRR334 pKa = 11.84CYY336 pKa = 10.64GRR338 pKa = 11.84RR339 pKa = 11.84NHH341 pKa = 6.92WGFICNRR348 pKa = 11.84HH349 pKa = 5.47RR350 pKa = 11.84RR351 pKa = 11.84QLDD354 pKa = 3.67PIITLLRR361 pKa = 11.84VRR363 pKa = 11.84QSSVRR368 pKa = 11.84RR369 pKa = 11.84RR370 pKa = 11.84VQRR373 pKa = 11.84RR374 pKa = 11.84AEE376 pKa = 4.03LSGHH380 pKa = 5.17VVDD383 pKa = 5.56GAMKK387 pKa = 10.93NEE389 pKa = 4.05VVWMNKK395 pKa = 8.51RR396 pKa = 11.84KK397 pKa = 7.95TRR399 pKa = 11.84AQVMALMLANRR410 pKa = 11.84MPTTVCGPFRR420 pKa = 11.84QRR422 pKa = 11.84HH423 pKa = 5.07LPAPPSFKK431 pKa = 10.45EE432 pKa = 4.05GCARR436 pKa = 3.88

Molecular weight: 49.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8561

26

8587

4345578

17

8214

506.1

56.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.202 ± 0.029

2.251 ± 0.014

5.066 ± 0.021

6.994 ± 0.048

3.677 ± 0.017

6.599 ± 0.025

2.499 ± 0.012

3.998 ± 0.017

4.616 ± 0.025

9.5 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.011

3.646 ± 0.016

4.868 ± 0.027

3.833 ± 0.027

7.011 ± 0.022

8.002 ± 0.032

5.671 ± 0.019

7.585 ± 0.024

1.109 ± 0.009

2.521 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski