Stachybotrys chlorohalonata (strain IBT 40285)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Stachybotryaceae; Stachybotrys; Stachybotrys chlorohalonata

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084QLP7|A0A084QLP7_STAC4 MHD domain-containing protein (Fragment) OS=Stachybotrys chlorohalonata (strain IBT 40285) OX=1283841 GN=S40285_02896 PE=4 SV=1
SS1 pKa = 6.49QLPNLLSLLSGLPGFPDD18 pKa = 3.15IATQTDD24 pKa = 4.09LPSIISALPNAPPALLPQLSTLFDD48 pKa = 4.46FLTQQPDD55 pKa = 3.62FPGILTGLPTPGLPLPTGLLSLAPPAASQLPNLLSLLSGLPGFPNIATQTDD106 pKa = 4.21LPSIISGLPAVPSGFLPQLPSLLDD130 pKa = 3.75FLTQQPDD137 pKa = 3.28LPGISTGLPTPGLPPTGLPSLAPSAASQLPNLLSLLSGLPGFPDD181 pKa = 3.06IATQIDD187 pKa = 4.51LPSIISGLPAVPSGLLPQLPTLFDD211 pKa = 3.94FLTRR215 pKa = 11.84QPNFPGILTGQPTPALPAFTGLPNIGSPVAGLPNFGFPSAPTLGGAQEE263 pKa = 4.29TEE265 pKa = 4.18LPKK268 pKa = 10.74VGSPTIPGWPNFGLPPFTGLPTLALPAASQLPNLLSILSGLPGFPNIATQTNLPSIISGLPNVPSGLLPQLPTLLDD344 pKa = 3.89FLTGQPNFPGILTAAPTFPGAPSFTGVPPVPGFPPLSGLPDD385 pKa = 3.76LPMPAMSQLPNLLNILSGLPGFPNIATQTNLPNIISGLPNVPSGLLPQLPTLLDD439 pKa = 3.89FLTGQPNFPGILTAAPTFPEE459 pKa = 4.74APSSTGLPNFGLPLASGLPPLTGLPGLPLSAISQLPNLLHH499 pKa = 6.15VLSGLPGFPNIATQTNLPSIVSGLPNAPSGLLPQLPTLLDD539 pKa = 3.68FLTRR543 pKa = 11.84QPNFPGVLTGTPTFPGLPTFPALPGFSGFPSAPGFPNIPGLPYY586 pKa = 10.76LPGLPTPPALPGPPGFPNIPGLPYY610 pKa = 10.76LPGLPTPPALPGFSGFPRR628 pKa = 11.84PPGFPNIPGLPYY640 pKa = 10.77LPDD643 pKa = 4.51LPTPPAFPSFPGFPGFPSPGGLPNKK668 pKa = 10.49LPGLPSASEE677 pKa = 4.42LPNQSGVPSIPGAPTFPSLPKK698 pKa = 10.31FSGPPTFPGLPNLPGLPNLPNPLPTALSQLPALLDD733 pKa = 3.78FLPGLPNFPEE743 pKa = 4.28VLTRR747 pKa = 11.84PSLPDD752 pKa = 3.89IISNAPNVPSGVLPQLPALLDD773 pKa = 3.81FLNGQPNFPGTPTSPPNLGNIFTGLPNLSPPAASQLPQLLNFLSEE818 pKa = 4.3LPSFPEE824 pKa = 3.97VLTQQALPSIISNAPNLPSGILPQLPEE851 pKa = 4.48LLNFLSGQPNFPAIPTGQPGLGSIFTGLPTLAPSAASQLPQLLSLLPSLPGFPNILTQPALPSIISNAPSVPSGVLPQLPEE932 pKa = 4.06LLNFLSQQPNFPGVLTGGLPGPGGIFNALPTLAPPAASQLPQLLNILPSLPGFPNILTQPALPSIISNAPNVPPGVLPEE1011 pKa = 4.33LPALLSFLSQQPNFPGILTGGPADD1035 pKa = 4.21LGSVFTGLPSLAPPAASQLPQLLSLLPSVPGFPNVLTASDD1075 pKa = 4.36LSSALAALPSLPPNLLTQLPTLLDD1099 pKa = 3.71FLTRR1103 pKa = 11.84QPNYY1107 pKa = 9.66PDD1109 pKa = 3.39IVTALPNFSLPAFVPAVPTSVPGMPQFPGIPTGGFQFPAAPTGVPGAPSFPGVPKK1164 pKa = 10.88APGKK1168 pKa = 9.64PSGVPAVPTGKK1179 pKa = 9.43PGTPGEE1185 pKa = 4.25PKK1187 pKa = 10.73LPVTNVLSGAPQFPGSPLFPSAPQLPEE1214 pKa = 4.54VPDD1217 pKa = 3.89KK1218 pKa = 10.37PTDD1221 pKa = 3.79PGAPQASDD1229 pKa = 3.64GADD1232 pKa = 3.57PLCPYY1237 pKa = 9.21PPWWEE1242 pKa = 3.87LQQPDD1247 pKa = 4.55DD1248 pKa = 5.27GVDD1251 pKa = 3.75EE1252 pKa = 4.38QCCCCDD1258 pKa = 2.48IWTGNIACRR1267 pKa = 11.84MLSGGCVCPAVEE1279 pKa = 4.72CAPGAPTVWPYY1290 pKa = 11.31GQDD1293 pKa = 2.89ATLPIATGIPGTDD1306 pKa = 3.55GYY1308 pKa = 11.05GIPAPP1313 pKa = 4.12

Molecular weight: 133.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084QN67|A0A084QN67_STAC4 Tryptophanyl-tRNA synthetase OS=Stachybotrys chlorohalonata (strain IBT 40285) OX=1283841 GN=S40285_00439 PE=3 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84WNGTRR7 pKa = 11.84TSSTLSSPTLTTRR20 pKa = 11.84RR21 pKa = 11.84PASAGSASGRR31 pKa = 11.84SRR33 pKa = 11.84GRR35 pKa = 11.84PSPRR39 pKa = 11.84TRR41 pKa = 11.84STTNHH46 pKa = 5.44RR47 pKa = 11.84RR48 pKa = 11.84TRR50 pKa = 11.84TII52 pKa = 2.95

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10676

0

10676

5257513

8

8218

492.5

54.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.075 ± 0.025

1.215 ± 0.01

5.889 ± 0.018

6.064 ± 0.023

3.623 ± 0.015

6.948 ± 0.024

2.445 ± 0.011

4.677 ± 0.018

4.45 ± 0.021

8.937 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.009

3.536 ± 0.012

6.135 ± 0.026

4.057 ± 0.016

6.308 ± 0.023

8.057 ± 0.024

5.891 ± 0.023

6.276 ± 0.018

1.488 ± 0.009

2.694 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski