Streptococcus phage Javan583

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6AJM9|A0A4D6AJM9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan583 OX=2548273 GN=Javan583_0044 PE=4 SV=1
MM1 pKa = 7.17QFDD4 pKa = 3.89EE5 pKa = 5.04VIEE8 pKa = 4.64FNDD11 pKa = 3.32EE12 pKa = 4.16LYY14 pKa = 10.82QKK16 pKa = 10.78NIADD20 pKa = 3.7HH21 pKa = 6.52TFPEE25 pKa = 5.29IDD27 pKa = 4.23DD28 pKa = 4.62LDD30 pKa = 4.41GKK32 pKa = 11.21GGTNEE37 pKa = 3.71

Molecular weight:
4.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6AJX1|A0A4D6AJX1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan583 OX=2548273 GN=Javan583_0022 PE=4 SV=1
MM1 pKa = 7.71AKK3 pKa = 10.24NGAKK7 pKa = 10.32GGGRR11 pKa = 11.84HH12 pKa = 5.47GAVKK16 pKa = 10.34GRR18 pKa = 11.84SQSYY22 pKa = 9.31NPKK25 pKa = 8.86TGLYY29 pKa = 7.82TKK31 pKa = 10.46RR32 pKa = 11.84NTSTGKK38 pKa = 10.51FMDD41 pKa = 3.49VKK43 pKa = 8.92TTGGKK48 pKa = 9.72FKK50 pKa = 11.02GVTSEE55 pKa = 4.15KK56 pKa = 10.95

Molecular weight:
5.91 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

9404

37

1387

165.0

18.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.401 ± 0.556

0.5 ± 0.108

5.859 ± 0.266

7.21 ± 0.543

3.86 ± 0.216

6.933 ± 0.577

1.404 ± 0.173

6.699 ± 0.301

7.614 ± 0.487

8.55 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.242

5.296 ± 0.346

2.882 ± 0.289

4.381 ± 0.309

4.849 ± 0.368

6.114 ± 0.475

6.285 ± 0.406

6.402 ± 0.303

1.223 ± 0.121

3.849 ± 0.297

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski