Amino acid dipepetide frequency for Hungateiclostridiaceae bacterium KB18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.726AlaAla: 9.726 ± 0.127	1.225AlaCys: 1.225 ± 0.036	4.403AlaAsp: 4.403 ± 0.069	6.038AlaGlu: 6.038 ± 0.08	3.159AlaPhe: 3.159 ± 0.058	7.394AlaGly: 7.394 ± 0.108	1.397AlaHis: 1.397 ± 0.032	4.404AlaIle: 4.404 ± 0.059	4.209AlaLys: 4.209 ± 0.068	9.251AlaLeu: 9.251 ± 0.111	2.461AlaMet: 2.461 ± 0.047	2.415AlaAsn: 2.415 ± 0.055	3.34AlaPro: 3.34 ± 0.071	3.054AlaGln: 3.054 ± 0.052	4.34AlaArg: 4.34 ± 0.076	3.851AlaSer: 3.851 ± 0.065	3.242AlaThr: 3.242 ± 0.082	7.056AlaVal: 7.056 ± 0.102	0.836AlaTrp: 0.836 ± 0.027	2.762AlaTyr: 2.762 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.323CysAla: 1.323 ± 0.038	0.345CysCys: 0.345 ± 0.019	0.871CysAsp: 0.871 ± 0.032	0.973CysGlu: 0.973 ± 0.028	0.642CysPhe: 0.642 ± 0.023	1.908CysGly: 1.908 ± 0.047	0.328CysHis: 0.328 ± 0.018	0.809CysIle: 0.809 ± 0.03	0.772CysLys: 0.772 ± 0.031	1.279CysLeu: 1.279 ± 0.032	0.402CysMet: 0.402 ± 0.019	0.483CysAsn: 0.483 ± 0.021	0.845CysPro: 0.845 ± 0.027	0.485CysGln: 0.485 ± 0.022	1.146CysArg: 1.146 ± 0.038	0.883CysSer: 0.883 ± 0.031	0.846CysThr: 0.846 ± 0.027	1.095CysVal: 1.095 ± 0.039	0.214CysTrp: 0.214 ± 0.014	0.537CysTyr: 0.537 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.77AspAla: 3.77 ± 0.059	0.929AspCys: 0.929 ± 0.032	2.45AspAsp: 2.45 ± 0.059	3.898AspGlu: 3.898 ± 0.07	2.792AspPhe: 2.792 ± 0.054	4.802AspGly: 4.802 ± 0.081	0.829AspHis: 0.829 ± 0.029	4.025AspIle: 4.025 ± 0.063	3.095AspLys: 3.095 ± 0.065	4.76AspLeu: 4.76 ± 0.072	1.837AspMet: 1.837 ± 0.04	1.964AspAsn: 1.964 ± 0.048	2.575AspPro: 2.575 ± 0.051	1.303AspGln: 1.303 ± 0.039	3.069AspArg: 3.069 ± 0.057	3.367AspSer: 3.367 ± 0.056	2.916AspThr: 2.916 ± 0.054	3.395AspVal: 3.395 ± 0.055	0.777AspTrp: 0.777 ± 0.024	2.353AspTyr: 2.353 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	5.424GluAla: 5.424 ± 0.082	0.822GluCys: 0.822 ± 0.029	4.301GluAsp: 4.301 ± 0.064	5.943GluGlu: 5.943 ± 0.087	2.542GluPhe: 2.542 ± 0.05	5.239GluGly: 5.239 ± 0.082	1.356GluHis: 1.356 ± 0.039	4.419GluIle: 4.419 ± 0.076	5.04GluLys: 5.04 ± 0.074	7.777GluLeu: 7.777 ± 0.105	2.088GluMet: 2.088 ± 0.038	3.438GluAsn: 3.438 ± 0.054	2.9GluPro: 2.9 ± 0.069	2.859GluGln: 2.859 ± 0.062	4.519GluArg: 4.519 ± 0.089	3.865GluSer: 3.865 ± 0.061	3.638GluThr: 3.638 ± 0.067	4.264GluVal: 4.264 ± 0.068	0.671GluTrp: 0.671 ± 0.028	2.913GluTyr: 2.913 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.315PheAla: 3.315 ± 0.059	0.848PheCys: 0.848 ± 0.027	2.593PheAsp: 2.593 ± 0.059	2.422PheGlu: 2.422 ± 0.051	1.763PhePhe: 1.763 ± 0.048	3.205PheGly: 3.205 ± 0.066	0.744PheHis: 0.744 ± 0.03	2.268PheIle: 2.268 ± 0.051	1.689PheLys: 1.689 ± 0.046	3.792PheLeu: 3.792 ± 0.071	1.031PheMet: 1.031 ± 0.031	1.369PheAsn: 1.369 ± 0.037	1.639PhePro: 1.639 ± 0.038	1.297PheGln: 1.297 ± 0.035	1.959PheArg: 1.959 ± 0.043	3.027PheSer: 3.027 ± 0.058	2.593PheThr: 2.593 ± 0.053	2.471PheVal: 2.471 ± 0.058	0.477PheTrp: 0.477 ± 0.024	1.554PheTyr: 1.554 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	6.335GlyAla: 6.335 ± 0.108	1.397GlyCys: 1.397 ± 0.038	3.931GlyAsp: 3.931 ± 0.07	5.623GlyGlu: 5.623 ± 0.081	3.322GlyPhe: 3.322 ± 0.062	6.358GlyGly: 6.358 ± 0.103	1.429GlyHis: 1.429 ± 0.037	4.684GlyIle: 4.684 ± 0.075	4.801GlyLys: 4.801 ± 0.086	7.439GlyLeu: 7.439 ± 0.096	2.47GlyMet: 2.47 ± 0.051	2.735GlyAsn: 2.735 ± 0.061	2.186GlyPro: 2.186 ± 0.051	2.58GlyGln: 2.58 ± 0.05	4.737GlyArg: 4.737 ± 0.071	4.561GlySer: 4.561 ± 0.066	4.263GlyThr: 4.263 ± 0.09	5.548GlyVal: 5.548 ± 0.077	1.0GlyTrp: 1.0 ± 0.037	3.221GlyTyr: 3.221 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.118HisAla: 1.118 ± 0.031	0.337HisCys: 0.337 ± 0.018	0.844HisAsp: 0.844 ± 0.026	1.049HisGlu: 1.049 ± 0.035	0.787HisPhe: 0.787 ± 0.028	1.476HisGly: 1.476 ± 0.039	0.399HisHis: 0.399 ± 0.021	1.285HisIle: 1.285 ± 0.035	0.853HisLys: 0.853 ± 0.032	1.551HisLeu: 1.551 ± 0.042	0.536HisMet: 0.536 ± 0.022	0.72HisAsn: 0.72 ± 0.025	0.947HisPro: 0.947 ± 0.031	0.555HisGln: 0.555 ± 0.022	1.087HisArg: 1.087 ± 0.038	1.033HisSer: 1.033 ± 0.033	0.994HisThr: 0.994 ± 0.03	0.991HisVal: 0.991 ± 0.032	0.253HisTrp: 0.253 ± 0.016	0.677HisTyr: 0.677 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.984IleAla: 4.984 ± 0.066	1.074IleCys: 1.074 ± 0.035	3.623IleAsp: 3.623 ± 0.065	3.91IleGlu: 3.91 ± 0.068	2.353IlePhe: 2.353 ± 0.049	4.022IleGly: 4.022 ± 0.06	0.989IleHis: 0.989 ± 0.032	3.483IleIle: 3.483 ± 0.068	2.964IleLys: 2.964 ± 0.061	5.326IleLeu: 5.326 ± 0.085	1.564IleMet: 1.564 ± 0.04	2.199IleAsn: 2.199 ± 0.042	2.547IlePro: 2.547 ± 0.051	1.611IleGln: 1.611 ± 0.044	3.017IleArg: 3.017 ± 0.056	4.195IleSer: 4.195 ± 0.06	3.536IleThr: 3.536 ± 0.065	3.745IleVal: 3.745 ± 0.075	0.57IleTrp: 0.57 ± 0.024	1.966IleTyr: 1.966 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.773LysAla: 4.773 ± 0.073	0.649LysCys: 0.649 ± 0.025	2.791LysAsp: 2.791 ± 0.054	4.639LysGlu: 4.639 ± 0.072	1.535LysPhe: 1.535 ± 0.037	3.692LysGly: 3.692 ± 0.062	0.877LysHis: 0.877 ± 0.028	3.027LysIle: 3.027 ± 0.06	3.782LysLys: 3.782 ± 0.064	5.305LysLeu: 5.305 ± 0.07	1.512LysMet: 1.512 ± 0.037	2.341LysAsn: 2.341 ± 0.052	2.185LysPro: 2.185 ± 0.049	1.718LysGln: 1.718 ± 0.049	3.246LysArg: 3.246 ± 0.06	3.018LysSer: 3.018 ± 0.058	2.916LysThr: 2.916 ± 0.066	3.337LysVal: 3.337 ± 0.061	0.6LysTrp: 0.6 ± 0.03	2.058LysTyr: 2.058 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	8.041LeuAla: 8.041 ± 0.1	2.113LeuCys: 2.113 ± 0.048	5.538LeuAsp: 5.538 ± 0.074	6.865LeuGlu: 6.865 ± 0.091	3.852LeuPhe: 3.852 ± 0.064	7.075LeuGly: 7.075 ± 0.104	1.79LeuHis: 1.79 ± 0.043	5.102LeuIle: 5.102 ± 0.074	4.881LeuLys: 4.881 ± 0.069	10.44LeuLeu: 10.44 ± 0.142	2.785LeuMet: 2.785 ± 0.055	3.279LeuAsn: 3.279 ± 0.072	4.841LeuPro: 4.841 ± 0.07	2.929LeuGln: 2.929 ± 0.059	5.82LeuArg: 5.82 ± 0.083	7.119LeuSer: 7.119 ± 0.088	5.492LeuThr: 5.492 ± 0.08	5.651LeuVal: 5.651 ± 0.073	1.004LeuTrp: 1.004 ± 0.031	3.35LeuTyr: 3.35 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.742MetAla: 2.742 ± 0.051	0.378MetCys: 0.378 ± 0.018	1.794MetAsp: 1.794 ± 0.042	2.358MetGlu: 2.358 ± 0.05	0.907MetPhe: 0.907 ± 0.027	2.167MetGly: 2.167 ± 0.05	0.377MetHis: 0.377 ± 0.019	1.325MetIle: 1.325 ± 0.035	1.884MetLys: 1.884 ± 0.049	2.771MetLeu: 2.771 ± 0.056	0.748MetMet: 0.748 ± 0.027	1.279MetAsn: 1.279 ± 0.036	1.241MetPro: 1.241 ± 0.036	0.879MetGln: 0.879 ± 0.03	1.595MetArg: 1.595 ± 0.039	1.762MetSer: 1.762 ± 0.039	1.5MetThr: 1.5 ± 0.037	1.764MetVal: 1.764 ± 0.043	0.253MetTrp: 0.253 ± 0.013	0.817MetTyr: 0.817 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.996AsnAla: 2.996 ± 0.058	0.576AsnCys: 0.576 ± 0.025	1.665AsnAsp: 1.665 ± 0.041	2.143AsnGlu: 2.143 ± 0.048	1.476AsnPhe: 1.476 ± 0.045	3.165AsnGly: 3.165 ± 0.067	0.662AsnHis: 0.662 ± 0.027	2.559AsnIle: 2.559 ± 0.051	1.623AsnLys: 1.623 ± 0.039	3.384AsnLeu: 3.384 ± 0.057	1.083AsnMet: 1.083 ± 0.033	1.32AsnAsn: 1.32 ± 0.038	1.872AsnPro: 1.872 ± 0.038	1.084AsnGln: 1.084 ± 0.034	1.93AsnArg: 1.93 ± 0.043	2.275AsnSer: 2.275 ± 0.049	2.024AsnThr: 2.024 ± 0.048	2.312AsnVal: 2.312 ± 0.046	0.442AsnTrp: 0.442 ± 0.021	1.356AsnTyr: 1.356 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	3.702ProAla: 3.702 ± 0.076	0.572ProCys: 0.572 ± 0.026	2.768ProAsp: 2.768 ± 0.06	4.563ProGlu: 4.563 ± 0.085	1.62ProPhe: 1.62 ± 0.042	3.653ProGly: 3.653 ± 0.061	0.688ProHis: 0.688 ± 0.026	2.157ProIle: 2.157 ± 0.045	2.173ProLys: 2.173 ± 0.05	3.473ProLeu: 3.473 ± 0.06	1.188ProMet: 1.188 ± 0.034	1.328ProAsn: 1.328 ± 0.037	1.582ProPro: 1.582 ± 0.051	1.51ProGln: 1.51 ± 0.039	1.833ProArg: 1.833 ± 0.047	2.266ProSer: 2.266 ± 0.05	2.083ProThr: 2.083 ± 0.058	3.363ProVal: 3.363 ± 0.061	0.436ProTrp: 0.436 ± 0.021	1.491ProTyr: 1.491 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.894GlnAla: 2.894 ± 0.061	0.403GlnCys: 0.403 ± 0.018	1.516GlnAsp: 1.516 ± 0.037	2.868GlnGlu: 2.868 ± 0.063	1.126GlnPhe: 1.126 ± 0.033	2.307GlnGly: 2.307 ± 0.049	0.521GlnHis: 0.521 ± 0.023	1.948GlnIle: 1.948 ± 0.046	2.199GlnLys: 2.199 ± 0.051	3.038GlnLeu: 3.038 ± 0.064	1.007GlnMet: 1.007 ± 0.028	1.361GlnAsn: 1.361 ± 0.042	1.279GlnPro: 1.279 ± 0.041	1.312GlnGln: 1.312 ± 0.04	2.168GlnArg: 2.168 ± 0.049	1.824GlnSer: 1.824 ± 0.041	1.577GlnThr: 1.577 ± 0.046	2.157GlnVal: 2.157 ± 0.048	0.402GlnTrp: 0.402 ± 0.02	1.224GlnTyr: 1.224 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	4.569ArgAla: 4.569 ± 0.075	0.806ArgCys: 0.806 ± 0.025	3.099ArgAsp: 3.099 ± 0.056	5.316ArgGlu: 5.316 ± 0.089	2.457ArgPhe: 2.457 ± 0.049	3.614ArgGly: 3.614 ± 0.067	1.041ArgHis: 1.041 ± 0.034	3.185ArgIle: 3.185 ± 0.063	2.994ArgLys: 2.994 ± 0.063	5.692ArgLeu: 5.692 ± 0.098	1.527ArgMet: 1.527 ± 0.041	1.931ArgAsn: 1.931 ± 0.038	2.259ArgPro: 2.259 ± 0.046	2.429ArgGln: 2.429 ± 0.055	4.06ArgArg: 4.06 ± 0.079	2.733ArgSer: 2.733 ± 0.06	2.579ArgThr: 2.579 ± 0.05	3.785ArgVal: 3.785 ± 0.059	0.724ArgTrp: 0.724 ± 0.027	2.193ArgTyr: 2.193 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.138SerAla: 5.138 ± 0.068	0.834SerCys: 0.834 ± 0.029	3.126SerAsp: 3.126 ± 0.055	4.036SerGlu: 4.036 ± 0.06	2.734SerPhe: 2.734 ± 0.053	5.754SerGly: 5.754 ± 0.082	1.062SerHis: 1.062 ± 0.035	3.263SerIle: 3.263 ± 0.054	2.711SerLys: 2.711 ± 0.051	5.641SerLeu: 5.641 ± 0.08	1.753SerMet: 1.753 ± 0.043	1.817SerAsn: 1.817 ± 0.045	2.535SerPro: 2.535 ± 0.053	2.039SerGln: 2.039 ± 0.044	3.407SerArg: 3.407 ± 0.063	3.613SerSer: 3.613 ± 0.078	2.808SerThr: 2.808 ± 0.056	4.279SerVal: 4.279 ± 0.067	0.655SerTrp: 0.655 ± 0.029	2.204SerTyr: 2.204 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	5.184ThrAla: 5.184 ± 0.091	0.611ThrCys: 0.611 ± 0.025	3.08ThrAsp: 3.08 ± 0.053	3.594ThrGlu: 3.594 ± 0.06	2.029ThrPhe: 2.029 ± 0.051	4.81ThrGly: 4.81 ± 0.107	0.899ThrHis: 0.899 ± 0.027	2.954ThrIle: 2.954 ± 0.064	2.292ThrLys: 2.292 ± 0.049	5.237ThrLeu: 5.237 ± 0.073	1.327ThrMet: 1.327 ± 0.035	1.532ThrAsn: 1.532 ± 0.049	2.659ThrPro: 2.659 ± 0.06	1.576ThrGln: 1.576 ± 0.044	2.348ThrArg: 2.348 ± 0.049	2.572ThrSer: 2.572 ± 0.049	2.442ThrThr: 2.442 ± 0.066	4.974ThrVal: 4.974 ± 0.094	0.494ThrTrp: 0.494 ± 0.022	1.835ThrTyr: 1.835 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.751ValAla: 4.751 ± 0.074	1.356ValCys: 1.356 ± 0.036	3.716ValAsp: 3.716 ± 0.054	4.503ValGlu: 4.503 ± 0.072	2.924ValPhe: 2.924 ± 0.056	4.371ValGly: 4.371 ± 0.074	1.116ValHis: 1.116 ± 0.03	4.037ValIle: 4.037 ± 0.069	3.724ValLys: 3.724 ± 0.066	7.098ValLeu: 7.098 ± 0.095	1.954ValMet: 1.954 ± 0.044	2.507ValAsn: 2.507 ± 0.052	3.137ValPro: 3.137 ± 0.058	2.093ValGln: 2.093 ± 0.042	3.697ValArg: 3.697 ± 0.065	4.634ValSer: 4.634 ± 0.065	4.229ValThr: 4.229 ± 0.084	4.377ValVal: 4.377 ± 0.085	0.725ValTrp: 0.725 ± 0.028	2.52ValTyr: 2.52 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.957TrpAla: 0.957 ± 0.029	0.204TrpCys: 0.204 ± 0.016	0.643TrpAsp: 0.643 ± 0.025	0.854TrpGlu: 0.854 ± 0.03	0.432TrpPhe: 0.432 ± 0.02	0.84TrpGly: 0.84 ± 0.029	0.267TrpHis: 0.267 ± 0.016	0.491TrpIle: 0.491 ± 0.024	0.493TrpLys: 0.493 ± 0.022	1.173TrpLeu: 1.173 ± 0.037	0.281TrpMet: 0.281 ± 0.017	0.468TrpAsn: 0.468 ± 0.02	0.346TrpPro: 0.346 ± 0.018	0.478TrpGln: 0.478 ± 0.023	0.73TrpArg: 0.73 ± 0.024	0.552TrpSer: 0.552 ± 0.025	0.551TrpThr: 0.551 ± 0.028	0.664TrpVal: 0.664 ± 0.028	0.152TrpTrp: 0.152 ± 0.012	0.492TrpTyr: 0.492 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.824TyrAla: 2.824 ± 0.053	0.708TyrCys: 0.708 ± 0.026	2.153TyrAsp: 2.153 ± 0.043	2.427TyrGlu: 2.427 ± 0.044	1.605TyrPhe: 1.605 ± 0.042	2.771TyrGly: 2.771 ± 0.054	0.723TyrHis: 0.723 ± 0.028	2.25TyrIle: 2.25 ± 0.049	1.822TyrLys: 1.822 ± 0.046	3.535TyrLeu: 3.535 ± 0.069	0.986TyrMet: 0.986 ± 0.031	1.461TyrAsn: 1.461 ± 0.034	1.534TyrPro: 1.534 ± 0.042	1.338TyrGln: 1.338 ± 0.037	2.286TyrArg: 2.286 ± 0.047	2.264TyrSer: 2.264 ± 0.052	2.218TyrThr: 2.218 ± 0.057	2.239TyrVal: 2.239 ± 0.046	0.383TyrTrp: 0.383 ± 0.02	1.528TyrTyr: 1.528 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3593 proteins (1066074 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---