Arthrobacter phage Noely

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KAD4|A0A3G2KAD4_9CAUD Minor tail protein OS=Arthrobacter phage Noely OX=2419964 GN=12 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.03EE3 pKa = 4.09HH4 pKa = 6.98GSLAPWPDD12 pKa = 4.09CLCGGPSWQEE22 pKa = 4.02CQDD25 pKa = 3.47GLAATARR32 pKa = 11.84AAGPPDD38 pKa = 4.33PPPLVTLVDD47 pKa = 3.67QARR50 pKa = 11.84IDD52 pKa = 3.64YY53 pKa = 8.97LFWRR57 pKa = 11.84GFDD60 pKa = 3.35TT61 pKa = 5.43

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KAF2|A0A3G2KAF2_9CAUD Helix-turn-helix DNA binding domain protein OS=Arthrobacter phage Noely OX=2419964 GN=3 PE=4 SV=1
MM1 pKa = 7.49NIKK4 pKa = 8.96PTDD7 pKa = 3.25KK8 pKa = 10.15MAAAGTGGAAALLLQWGARR27 pKa = 11.84QAGLDD32 pKa = 4.03LPPDD36 pKa = 3.42VAAAFVLVAAFVAGWLRR53 pKa = 11.84TEE55 pKa = 4.35GGRR58 pKa = 11.84RR59 pKa = 11.84EE60 pKa = 4.06GRR62 pKa = 11.84HH63 pKa = 5.59RR64 pKa = 11.84GG65 pKa = 3.39

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

4603

47

558

200.1

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.86 ± 0.599

0.608 ± 0.164

6.691 ± 0.328

5.301 ± 0.553

1.955 ± 0.126

9.255 ± 0.398

1.369 ± 0.205

2.955 ± 0.323

4.236 ± 0.445

8.799 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.262

2.911 ± 0.332

5.279 ± 0.582

3.91 ± 0.302

6.604 ± 0.566

4.541 ± 0.316

6.517 ± 0.369

8.038 ± 0.493

1.825 ± 0.192

2.194 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski