Orchesella cincta (Springtail)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Collembola; Entomobryomorpha; Entomobryoidea; Orchesellidae; Orchesellinae; Orchesella

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2N0T3|A0A1D2N0T3_ORCCI Uncharacterized protein OS=Orchesella cincta OX=48709 GN=Ocin01_07865 PE=4 SV=1
MM1 pKa = 7.49NSFSFALIFGLVAISQAQVPTLIEE25 pKa = 3.96STLDD29 pKa = 3.49GKK31 pKa = 11.01VLTITNKK38 pKa = 8.77TVPPTGPYY46 pKa = 9.6QRR48 pKa = 11.84LGVAALDD55 pKa = 3.76VDD57 pKa = 4.11NSQGFPQYY65 pKa = 9.47WAFDD69 pKa = 3.72VFGGDD74 pKa = 3.26INRR77 pKa = 11.84GQIGPVNGVVWNSLTAYY94 pKa = 9.49LPCDD98 pKa = 3.13DD99 pKa = 4.79CFAYY103 pKa = 9.85FYY105 pKa = 10.13TYY107 pKa = 11.24DD108 pKa = 3.47FGQIVSNGSRR118 pKa = 11.84LLKK121 pKa = 10.64RR122 pKa = 11.84IDD124 pKa = 3.47TDD126 pKa = 3.52SCVQNPGNGGFITEE140 pKa = 4.5ATCNEE145 pKa = 4.19TDD147 pKa = 4.52VSQLWIINNDD157 pKa = 3.8SITTNVNHH165 pKa = 7.55DD166 pKa = 4.41SIEE169 pKa = 4.49DD170 pKa = 3.5NSVSSEE176 pKa = 3.68EE177 pKa = 4.13LNIGEE182 pKa = 4.94HH183 pKa = 6.4KK184 pKa = 10.75SSSTSDD190 pKa = 3.57EE191 pKa = 3.89QSSASTSDD199 pKa = 3.06EE200 pKa = 4.47DD201 pKa = 3.77NN202 pKa = 3.51

Molecular weight: 21.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2MTQ8|A0A1D2MTQ8_ORCCI Chromodomain-helicase-DNA-binding protein 7 (Fragment) OS=Orchesella cincta OX=48709 GN=Ocin01_10391 PE=4 SV=1
MM1 pKa = 7.47SAHH4 pKa = 5.52KK5 pKa = 8.98TLRR8 pKa = 11.84IKK10 pKa = 10.75RR11 pKa = 11.84KK12 pKa = 8.86LAKK15 pKa = 10.19KK16 pKa = 9.11MNQNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20218

0

20218

8574862

9

8408

424.1

47.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.114 ± 0.013

1.991 ± 0.017

5.333 ± 0.012

6.512 ± 0.018

4.261 ± 0.013

6.101 ± 0.021

2.447 ± 0.01

5.566 ± 0.012

6.345 ± 0.021

8.873 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.007

4.936 ± 0.012

5.239 ± 0.019

4.011 ± 0.015

5.138 ± 0.013

8.429 ± 0.026

5.75 ± 0.015

6.294 ± 0.012

1.2 ± 0.006

3.107 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski