Sulfolobus polyhedral virus 1

Taxonomy: Viruses; Portogloboviridae; Alphaportoglobovirus; Sulfolobus alphaportoglobovirus 1

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6I169|A0A1W6I169_9VIRU Uncharacterized protein OS=Sulfolobus polyhedral virus 1 OX=1982658 PE=4 SV=1
MM1 pKa = 8.02SEE3 pKa = 4.71DD4 pKa = 3.5DD5 pKa = 4.61CSFCRR10 pKa = 11.84SIMEE14 pKa = 4.42NGEE17 pKa = 4.07EE18 pKa = 3.92EE19 pKa = 4.56QIWVLIDD26 pKa = 4.53LICDD30 pKa = 3.4NFDD33 pKa = 3.63EE34 pKa = 5.87FSSEE38 pKa = 3.84CDD40 pKa = 3.09KK41 pKa = 10.95IYY43 pKa = 11.27SMEE46 pKa = 4.36AKK48 pKa = 10.09PIVDD52 pKa = 3.25INSAIKK58 pKa = 10.67LIVGKK63 pKa = 10.57AKK65 pKa = 10.84LIDD68 pKa = 3.9NCSSLAQFIFDD79 pKa = 3.66VVDD82 pKa = 4.62FGG84 pKa = 4.79

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6I182|A0A1W6I182_9VIRU Uncharacterized protein OS=Sulfolobus polyhedral virus 1 OX=1982658 PE=4 SV=1
MM1 pKa = 6.89FTDD4 pKa = 5.16LKK6 pKa = 10.79FSKK9 pKa = 10.65NLTAYY14 pKa = 10.0RR15 pKa = 11.84QTTYY19 pKa = 10.54RR20 pKa = 11.84QTPGTQSKK28 pKa = 10.89NSLEE32 pKa = 3.93NSVYY36 pKa = 10.07IQYY39 pKa = 10.06TYY41 pKa = 10.1TQTHH45 pKa = 6.11RR46 pKa = 11.84QTKK49 pKa = 8.3YY50 pKa = 9.27RR51 pKa = 11.84QTRR54 pKa = 11.84RR55 pKa = 11.84QTHH58 pKa = 4.67QQTNPPTTDD67 pKa = 2.96TQFF70 pKa = 3.39

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

6008

34

400

133.5

14.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.925 ± 0.352

1.215 ± 0.196

4.361 ± 0.416

5.476 ± 0.646

3.612 ± 0.257

6.009 ± 0.525

1.115 ± 0.202

7.473 ± 0.345

5.959 ± 0.766

9.587 ± 0.423

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.254

7.041 ± 0.453

3.911 ± 0.447

4.627 ± 0.442

2.83 ± 0.456

7.989 ± 0.697

7.39 ± 0.752

6.009 ± 0.39

0.849 ± 0.127

6.042 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski