Alteromonadaceae bacterium 2753L.S.0a.02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; unclassified Alteromonadaceae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559QPU8|A0A559QPU8_9ALTE Acetate kinase OS=Alteromonadaceae bacterium 2753L.S.0a.02 OX=1403320 GN=ackA PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.36FVFTTIARR10 pKa = 11.84LLGLSALTVSLPALATTVQFQTSMGDD36 pKa = 3.62FEE38 pKa = 5.21VVLYY42 pKa = 10.73DD43 pKa = 3.14QGTPATVANFLAYY56 pKa = 10.34VEE58 pKa = 4.77AGDD61 pKa = 3.82YY62 pKa = 10.05TDD64 pKa = 4.52TIIHH68 pKa = 6.59RR69 pKa = 11.84SVDD72 pKa = 3.34SFVIQGGGYY81 pKa = 9.59VFAEE85 pKa = 4.54SGAPDD90 pKa = 5.23AIDD93 pKa = 3.21TRR95 pKa = 11.84DD96 pKa = 3.53PVVNEE101 pKa = 3.67PVYY104 pKa = 11.01SNVRR108 pKa = 11.84GTIAMAKK115 pKa = 10.13VSGLLNSATSQWFINLSNNSFSLDD139 pKa = 3.3PYY141 pKa = 10.24NRR143 pKa = 11.84GGFTVFGEE151 pKa = 4.35VAEE154 pKa = 4.71GGMSIIDD161 pKa = 3.93SIAAVDD167 pKa = 4.02VFDD170 pKa = 4.44MGTGFDD176 pKa = 4.66EE177 pKa = 4.94IPLVDD182 pKa = 4.11YY183 pKa = 11.07TEE185 pKa = 4.31EE186 pKa = 4.71DD187 pKa = 4.08YY188 pKa = 11.85NNQIDD193 pKa = 4.07PTADD197 pKa = 3.24NAVTVYY203 pKa = 10.11TIVVTDD209 pKa = 4.86ADD211 pKa = 4.31PDD213 pKa = 3.89SAADD217 pKa = 4.38LDD219 pKa = 4.12PVPNNLYY226 pKa = 10.34EE227 pKa = 4.55EE228 pKa = 4.55PTPTPTPTPSSGDD241 pKa = 3.28GGGGGFPYY249 pKa = 11.02ALLLLLGGLSLVARR263 pKa = 11.84KK264 pKa = 9.69GSHH267 pKa = 5.6NSS269 pKa = 3.06

Molecular weight: 28.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559QVC9|A0A559QVC9_9ALTE Threonine/homoserine/homoserine lactone efflux protein OS=Alteromonadaceae bacterium 2753L.S.0a.02 OX=1403320 GN=P886_1179 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.64VLAAA44 pKa = 4.31

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4947

0

4947

1758315

19

7441

355.4

39.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.009 ± 0.037

1.134 ± 0.016

5.609 ± 0.041

6.141 ± 0.035

4.137 ± 0.022

7.008 ± 0.043

2.183 ± 0.019

5.818 ± 0.026

4.584 ± 0.034

10.25 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.02

4.559 ± 0.029

4.162 ± 0.024

4.324 ± 0.027

4.943 ± 0.029

7.182 ± 0.054

5.377 ± 0.033

6.798 ± 0.028

1.376 ± 0.016

3.232 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski