Mycoplasma pulmonis (strain UAB CTIP)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis pulmonis

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q98PW0|RS18_MYCPU 30S ribosomal protein S18 OS=Mycoplasma pulmonis (strain UAB CTIP) OX=272635 GN=rpsR PE=3 SV=1
MM1 pKa = 7.39NMSLDD6 pKa = 3.86DD7 pKa = 4.94DD8 pKa = 4.12FLKK11 pKa = 11.19VVDD14 pKa = 4.69LHH16 pKa = 8.04GLDD19 pKa = 3.54QQDD22 pKa = 3.62ALGKK26 pKa = 10.16IINLLFDD33 pKa = 5.02LEE35 pKa = 4.36NSDD38 pKa = 3.86DD39 pKa = 3.92QKK41 pKa = 11.79VLVITGRR48 pKa = 11.84GYY50 pKa = 11.35GVLKK54 pKa = 9.22QTLEE58 pKa = 4.16QILDD62 pKa = 3.71EE63 pKa = 3.85QDD65 pKa = 3.25YY66 pKa = 11.09YY67 pKa = 11.86YY68 pKa = 10.39EE69 pKa = 4.07IQNNGGAYY77 pKa = 10.53LIYY80 pKa = 10.44AKK82 pKa = 10.4HH83 pKa = 6.15EE84 pKa = 4.08KK85 pKa = 9.93TFDD88 pKa = 3.21FDD90 pKa = 5.51LIEE93 pKa = 5.53DD94 pKa = 4.1YY95 pKa = 10.99EE96 pKa = 4.2

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q98PZ8|RL18_MYCPU 50S ribosomal protein L18 OS=Mycoplasma pulmonis (strain UAB CTIP) OX=272635 GN=rplR PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.19KK4 pKa = 10.15SLKK7 pKa = 10.21VKK9 pKa = 10.01AARR12 pKa = 11.84VAKK15 pKa = 10.27FSTRR19 pKa = 11.84AYY21 pKa = 8.8TRR23 pKa = 11.84CQLCGRR29 pKa = 11.84PHH31 pKa = 5.94AVLRR35 pKa = 11.84KK36 pKa = 9.33FEE38 pKa = 3.78ICRR41 pKa = 11.84ICFRR45 pKa = 11.84KK46 pKa = 9.48LAHH49 pKa = 6.58EE50 pKa = 4.43GKK52 pKa = 10.17IPGLKK57 pKa = 9.11KK58 pKa = 10.82ASWW61 pKa = 3.28

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

778

0

778

288921

37

3216

371.4

42.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.694 ± 0.07

0.302 ± 0.015

5.352 ± 0.064

6.895 ± 0.093

6.104 ± 0.087

4.499 ± 0.091

1.412 ± 0.03

9.481 ± 0.115

11.002 ± 0.105

9.571 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.596 ± 0.045

7.619 ± 0.112

2.707 ± 0.069

3.676 ± 0.07

2.828 ± 0.05

7.602 ± 0.095

4.667 ± 0.114

5.301 ± 0.072

0.947 ± 0.03

3.743 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski