Pontibacillus halophilus JSM 076056 = DSM 19796

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pontibacillus; Pontibacillus halophilus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A5GEA1|A0A0A5GEA1_9BACI NADH-dependent butanol dehydrogenase A OS=Pontibacillus halophilus JSM 076056 = DSM 19796 OX=1385510 GN=N781_07500 PE=4 SV=1
MM1 pKa = 7.45AFVNRR6 pKa = 11.84FSTLDD11 pKa = 3.4CGMMTFTGNTLGLSQEE27 pKa = 4.33LNLNEE32 pKa = 4.24AGTRR36 pKa = 11.84GSIGAFITLDD46 pKa = 3.36NTSQVPTFPPGTTLNYY62 pKa = 10.16QEE64 pKa = 4.71NSSSAEE70 pKa = 3.81LGFAPGSNTVLYY82 pKa = 10.91AEE84 pKa = 5.47LIWGGNYY91 pKa = 7.55LTRR94 pKa = 11.84DD95 pKa = 3.25EE96 pKa = 6.13DD97 pKa = 3.56ITSEE101 pKa = 3.87IDD103 pKa = 3.18NDD105 pKa = 4.11VLFTDD110 pKa = 4.64PLGVAHH116 pKa = 7.52PISPDD121 pKa = 3.39PATSNQFTFTIGGVTRR137 pKa = 11.84GFYY140 pKa = 9.49MRR142 pKa = 11.84SNDD145 pKa = 3.14VTSIVQSAAEE155 pKa = 4.08GTYY158 pKa = 9.5TCGSVPGLLDD168 pKa = 3.66PLIASTGDD176 pKa = 3.27TNHH179 pKa = 7.11AGWTLAVVYY188 pKa = 9.1GNPEE192 pKa = 3.81LPNRR196 pKa = 11.84SMNLFVGGEE205 pKa = 4.39GIVFSQGTPIIDD217 pKa = 3.34IAVSGFMTPPSGDD230 pKa = 2.59VDD232 pKa = 3.5ARR234 pKa = 11.84LLISAQEE241 pKa = 3.93GDD243 pKa = 3.68ANIPGDD249 pKa = 3.6QALFGPDD256 pKa = 3.78SLTLTNLSGPNNPTFNFSASQINGDD281 pKa = 4.41DD282 pKa = 3.93GLVDD286 pKa = 3.37TSGTFGTRR294 pKa = 11.84NQDD297 pKa = 2.74AATQTNIVAGRR308 pKa = 11.84QGWDD312 pKa = 2.85ITNVSGLNYY321 pKa = 10.25LPNNQTTAVFRR332 pKa = 11.84FTSTGDD338 pKa = 3.04AYY340 pKa = 10.65MPNALGIQIDD350 pKa = 4.21EE351 pKa = 4.48GDD353 pKa = 4.07ADD355 pKa = 3.99PVLVKK360 pKa = 10.04TVDD363 pKa = 3.15KK364 pKa = 10.7PFAVVNDD371 pKa = 3.41VLTYY375 pKa = 10.36IITITNEE382 pKa = 3.79GVIEE386 pKa = 4.22ANNVVFIDD394 pKa = 4.79EE395 pKa = 4.81IPPGTLFVEE404 pKa = 4.7DD405 pKa = 3.83SLYY408 pKa = 10.26IDD410 pKa = 4.92GVQFPGVNPQFGVPLPNIDD429 pKa = 3.51VGDD432 pKa = 3.84TVTVFFQVIVRR443 pKa = 11.84RR444 pKa = 11.84CDD446 pKa = 3.39CFVQNRR452 pKa = 11.84AEE454 pKa = 4.43LEE456 pKa = 4.19FSCGKK461 pKa = 9.82VAEE464 pKa = 4.7SNLVTSTVCFQCYY477 pKa = 9.37CGSGFICC484 pKa = 5.34

Molecular weight: 51.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A5GP05|A0A0A5GP05_9BACI Aspartate racemase OS=Pontibacillus halophilus JSM 076056 = DSM 19796 OX=1385510 GN=N781_00460 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.1NGRR28 pKa = 11.84EE29 pKa = 3.63VLKK32 pKa = 10.46RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.96GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3559

0

3559

1015912

26

2105

285.4

32.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.855 ± 0.038

0.592 ± 0.013

5.276 ± 0.037

8.051 ± 0.059

4.371 ± 0.038

7.041 ± 0.041

2.343 ± 0.025

6.743 ± 0.037

5.672 ± 0.033

9.755 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.019

3.981 ± 0.028

3.659 ± 0.024

4.188 ± 0.027

4.398 ± 0.032

6.192 ± 0.032

5.727 ± 0.03

7.516 ± 0.036

1.085 ± 0.018

3.696 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski