Duganella sp. CF402

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Duganella; unclassified Duganella

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5435 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8AE70|A0A1H8AE70_9BURK Cytochrome c554 and c-prime OS=Duganella sp. CF402 OX=1855289 GN=SAMN05216319_4480 PE=4 SV=1
MM1 pKa = 7.64TSPALLSVSSSDD13 pKa = 2.95NSTIVLHH20 pKa = 6.52FDD22 pKa = 3.4RR23 pKa = 11.84NVFAGDD29 pKa = 3.43GSIVVSDD36 pKa = 4.84GYY38 pKa = 11.52GQTFLSDD45 pKa = 2.69GGLRR49 pKa = 11.84TRR51 pKa = 11.84IVNASDD57 pKa = 3.37QRR59 pKa = 11.84ALDD62 pKa = 4.09ASDD65 pKa = 3.85SALTYY70 pKa = 10.22SGQNITIHH78 pKa = 7.01LSTPLKK84 pKa = 10.79NGLNYY89 pKa = 10.25SITMSGGTVIDD100 pKa = 5.0DD101 pKa = 4.03SDD103 pKa = 4.63DD104 pKa = 4.29GIARR108 pKa = 11.84ISSPNLFKK116 pKa = 10.42FTASGSAATVPTPSAVIGSTLHH138 pKa = 5.53FTDD141 pKa = 4.13TGTSSSDD148 pKa = 3.65YY149 pKa = 8.87ITASASQVMTGTYY162 pKa = 9.5TGTLGASDD170 pKa = 4.49FVQVSLDD177 pKa = 4.12NGASWHH183 pKa = 6.24KK184 pKa = 10.18ATVNASAKK192 pKa = 7.34TWSYY196 pKa = 10.4TGEE199 pKa = 3.98IDD201 pKa = 5.73LDD203 pKa = 3.94NLTDD207 pKa = 3.93GAGDD211 pKa = 3.95ALDD214 pKa = 3.95GTLLARR220 pKa = 11.84IGNTSGGSSGTASHH234 pKa = 6.47TYY236 pKa = 9.95VYY238 pKa = 10.52NSHH241 pKa = 6.52AVEE244 pKa = 4.38IDD246 pKa = 3.43VSSSFSFSADD256 pKa = 2.85TGTSSTDD263 pKa = 4.02LITKK267 pKa = 8.01TAAQTISGSYY277 pKa = 9.85SGSLLAGYY285 pKa = 6.8TLQVSVDD292 pKa = 3.87GGANWINASASAGSWHH308 pKa = 5.58TTGTVNLKK316 pKa = 10.43SGANEE321 pKa = 3.59VQARR325 pKa = 11.84VIDD328 pKa = 3.93TAGNTSGVASADD340 pKa = 3.49YY341 pKa = 11.02KK342 pKa = 11.21LITSTVSLAGHH353 pKa = 7.46ALTLAEE359 pKa = 4.98GSDD362 pKa = 3.99TGPSATDD369 pKa = 4.88GITRR373 pKa = 11.84SASKK377 pKa = 9.23VTLNVAGLHH386 pKa = 6.02GFHH389 pKa = 7.12AGDD392 pKa = 4.44TIQIVDD398 pKa = 3.99TSHH401 pKa = 6.52SSAVVGSYY409 pKa = 10.84VILDD413 pKa = 3.44ADD415 pKa = 4.59LYY417 pKa = 11.46YY418 pKa = 11.17GDD420 pKa = 5.79DD421 pKa = 3.73YY422 pKa = 11.38FTISKK427 pKa = 9.24FNPTARR433 pKa = 11.84TTVDD437 pKa = 2.86INLDD441 pKa = 3.52TMADD445 pKa = 3.83GAHH448 pKa = 5.99TLAARR453 pKa = 11.84IVDD456 pKa = 3.8VAGNTAAASGTTAVTVDD473 pKa = 3.36GKK475 pKa = 11.12VPLMLSSAPLEE486 pKa = 4.56DD487 pKa = 3.3ATGVSTGLTKK497 pKa = 10.16LTFTFNEE504 pKa = 4.23NIAIEE509 pKa = 4.86DD510 pKa = 3.84GTIVTITDD518 pKa = 4.04DD519 pKa = 3.83NNSANSQEE527 pKa = 4.04ITLSSTAASGNKK539 pKa = 8.23LTINLSSALTSGTSYY554 pKa = 10.37TVIGAIVTDD563 pKa = 4.09LAGNDD568 pKa = 3.86GVTGDD573 pKa = 3.85TPLLHH578 pKa = 6.42FTTEE582 pKa = 3.56GTYY585 pKa = 10.79AGGSTPDD592 pKa = 3.51SVAADD597 pKa = 3.91YY598 pKa = 11.71VDD600 pKa = 4.44TAPAWDD606 pKa = 3.98TDD608 pKa = 4.05SGSDD612 pKa = 3.3AHH614 pKa = 6.97NDD616 pKa = 3.09GVTSNTRR623 pKa = 11.84ISVTGAPLTEE633 pKa = 3.51WHH635 pKa = 6.54YY636 pKa = 11.45SVNGTTYY643 pKa = 10.59LGSGDD648 pKa = 4.4GFDD651 pKa = 5.68LEE653 pKa = 5.36DD654 pKa = 3.39GTYY657 pKa = 9.31TAGQIQVWQTVDD669 pKa = 3.52GVDD672 pKa = 3.81SAHH675 pKa = 5.94TSIGKK680 pKa = 7.87TLVIDD685 pKa = 4.04TLAPTAVAHH694 pKa = 6.16GVPEE698 pKa = 4.37SFSSGDD704 pKa = 3.43SSIEE708 pKa = 3.8GLVMGTTDD716 pKa = 3.04VAEE719 pKa = 4.39EE720 pKa = 4.03FVEE723 pKa = 4.52VTFDD727 pKa = 4.7HH728 pKa = 6.73GVTWVQAEE736 pKa = 4.34TTYY739 pKa = 11.49VNADD743 pKa = 3.26YY744 pKa = 9.44STWSLAGISASLGNNYY760 pKa = 9.45GLRR763 pKa = 11.84LSDD766 pKa = 3.21TAGNISTFNALSATNPVYY784 pKa = 10.87YY785 pKa = 10.42LADD788 pKa = 3.88GGVTYY793 pKa = 10.86SHH795 pKa = 7.22ASDD798 pKa = 5.51DD799 pKa = 3.91YY800 pKa = 8.31TTVFGGSGADD810 pKa = 3.61TITVGAHH817 pKa = 5.71ALVAGGDD824 pKa = 3.63GDD826 pKa = 5.59IITTGANSAIITRR839 pKa = 11.84ASSTVVTGGGTNVVEE854 pKa = 4.67ADD856 pKa = 3.7SNAHH860 pKa = 5.61ITTGSGTDD868 pKa = 3.69SITLTSLTGAVLAAGSGTDD887 pKa = 3.47TLILEE892 pKa = 4.57FDD894 pKa = 3.82GSFTLGTALGSSSGIDD910 pKa = 3.08IIDD913 pKa = 4.07FGSDD917 pKa = 2.98GTGHH921 pKa = 6.01MNILTSASVHH931 pKa = 5.99NLTDD935 pKa = 3.71SDD937 pKa = 3.91QLTVNDD943 pKa = 4.24SGGGATTMTLEE954 pKa = 4.2SLVWAHH960 pKa = 6.54TGTSGAYY967 pKa = 9.04EE968 pKa = 3.96IYY970 pKa = 10.52KK971 pKa = 8.71NTVSGGDD978 pKa = 3.4GTVVVLIANTIDD990 pKa = 3.31VTLDD994 pKa = 2.94TFTAA998 pKa = 3.89

Molecular weight: 101.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8E1C6|A0A1H8E1C6_9BURK Cholesterol transport system auxiliary component OS=Duganella sp. CF402 OX=1855289 GN=SAMN05216319_5389 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84QVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5435

0

5435

1935185

33

5078

356.1

38.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.503 ± 0.054

0.794 ± 0.011

5.42 ± 0.03

5.102 ± 0.034

3.518 ± 0.021

8.063 ± 0.046

2.151 ± 0.017

4.828 ± 0.021

3.913 ± 0.03

10.47 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.015

3.309 ± 0.027

4.795 ± 0.027

4.217 ± 0.029

5.955 ± 0.034

5.791 ± 0.031

5.415 ± 0.039

7.231 ± 0.028

1.344 ± 0.015

2.684 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski