Saccharopolyspora shandongensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3MJL6|A0A1H3MJL6_9PSEU Assimilatory sulfite reductase (ferredoxin) OS=Saccharopolyspora shandongensis OX=418495 GN=SAMN05216215_103465 PE=4 SV=1
MM1 pKa = 7.36SWTIEE6 pKa = 3.89VDD8 pKa = 2.92ADD10 pKa = 3.79TCIGSGMCAGVAPDD24 pKa = 3.89HH25 pKa = 6.73FEE27 pKa = 4.39LVDD30 pKa = 4.1GYY32 pKa = 11.73GCARR36 pKa = 11.84KK37 pKa = 9.16AAVEE41 pKa = 4.02PADD44 pKa = 3.7QVIDD48 pKa = 3.72AAEE51 pKa = 4.26SCPVEE56 pKa = 4.8AILVKK61 pKa = 10.74DD62 pKa = 3.78ADD64 pKa = 4.13TGDD67 pKa = 3.44VVAPEE72 pKa = 4.07EE73 pKa = 4.11

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3JCZ3|A0A1H3JCZ3_9PSEU Cytochrome c biogenesis protein OS=Saccharopolyspora shandongensis OX=418495 GN=SAMN05216215_1025113 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9219

0

9219

2930089

24

17456

317.8

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.188 ± 0.038

0.811 ± 0.012

6.036 ± 0.025

5.899 ± 0.03

2.949 ± 0.017

9.115 ± 0.025

2.286 ± 0.017

3.691 ± 0.02

1.972 ± 0.017

10.407 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.013

1.978 ± 0.013

5.896 ± 0.024

3.117 ± 0.02

7.976 ± 0.031

5.224 ± 0.026

5.592 ± 0.024

8.597 ± 0.03

1.531 ± 0.011

1.903 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski