Amino acid dipepetide frequency for Escherichia phage Lys12581Vzw

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.683AlaAla: 8.683 ± 0.796
0.852AlaCys: 0.852 ± 0.331
5.593AlaAsp: 5.593 ± 0.574
7.724AlaGlu: 7.724 ± 0.643
3.729AlaPhe: 3.729 ± 0.492
7.937AlaGly: 7.937 ± 1.105
1.278AlaHis: 1.278 ± 0.269
3.942AlaIle: 3.942 ± 0.516
5.167AlaLys: 5.167 ± 0.496
7.032AlaLeu: 7.032 ± 0.644
3.036AlaMet: 3.036 ± 0.417
2.77AlaAsn: 2.77 ± 0.388
3.196AlaPro: 3.196 ± 0.452
4.635AlaGln: 4.635 ± 0.737
5.966AlaArg: 5.966 ± 0.623
5.86AlaSer: 5.86 ± 0.587
5.007AlaThr: 5.007 ± 0.732
7.192AlaVal: 7.192 ± 0.552
1.651AlaTrp: 1.651 ± 0.274
2.45AlaTyr: 2.45 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
0.906CysAla: 0.906 ± 0.271
0.213CysCys: 0.213 ± 0.105
0.746CysAsp: 0.746 ± 0.229
0.746CysGlu: 0.746 ± 0.258
0.479CysPhe: 0.479 ± 0.16
0.906CysGly: 0.906 ± 0.316
0.213CysHis: 0.213 ± 0.113
0.479CysIle: 0.479 ± 0.168
0.426CysLys: 0.426 ± 0.183
1.012CysLeu: 1.012 ± 0.232
0.16CysMet: 0.16 ± 0.092
0.479CysAsn: 0.479 ± 0.195
0.639CysPro: 0.639 ± 0.219
0.266CysGln: 0.266 ± 0.128
0.852CysArg: 0.852 ± 0.245
0.906CysSer: 0.906 ± 0.279
0.32CysThr: 0.32 ± 0.156
1.065CysVal: 1.065 ± 0.256
0.16CysTrp: 0.16 ± 0.084
0.266CysTyr: 0.266 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.807AspAla: 5.807 ± 0.618
0.479AspCys: 0.479 ± 0.18
3.729AspAsp: 3.729 ± 0.468
4.581AspGlu: 4.581 ± 0.51
1.864AspPhe: 1.864 ± 0.292
4.848AspGly: 4.848 ± 0.525
0.852AspHis: 0.852 ± 0.294
3.303AspIle: 3.303 ± 0.435
4.421AspLys: 4.421 ± 0.439
4.155AspLeu: 4.155 ± 0.44
1.492AspMet: 1.492 ± 0.236
2.397AspAsn: 2.397 ± 0.405
2.184AspPro: 2.184 ± 0.415
1.385AspGln: 1.385 ± 0.338
3.676AspArg: 3.676 ± 0.446
2.61AspSer: 2.61 ± 0.377
3.463AspThr: 3.463 ± 0.387
4.421AspVal: 4.421 ± 0.379
0.852AspTrp: 0.852 ± 0.291
1.598AspTyr: 1.598 ± 0.31
0.0AspXaa: 0.0 ± 0.0
Glu
6.233GluAla: 6.233 ± 0.549
0.906GluCys: 0.906 ± 0.269
2.397GluAsp: 2.397 ± 0.363
4.208GluGlu: 4.208 ± 0.456
2.983GluPhe: 2.983 ± 0.454
3.835GluGly: 3.835 ± 0.532
1.758GluHis: 1.758 ± 0.39
3.782GluIle: 3.782 ± 0.446
4.741GluLys: 4.741 ± 0.524
5.807GluLeu: 5.807 ± 0.594
2.078GluMet: 2.078 ± 0.339
2.664GluAsn: 2.664 ± 0.387
2.078GluPro: 2.078 ± 0.371
4.049GluGln: 4.049 ± 0.517
5.114GluArg: 5.114 ± 0.573
3.729GluSer: 3.729 ± 0.401
3.889GluThr: 3.889 ± 0.691
4.688GluVal: 4.688 ± 0.601
0.852GluTrp: 0.852 ± 0.173
2.291GluTyr: 2.291 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
3.25PheAla: 3.25 ± 0.426
0.746PheCys: 0.746 ± 0.198
2.237PheAsp: 2.237 ± 0.353
1.545PheGlu: 1.545 ± 0.301
1.172PhePhe: 1.172 ± 0.252
2.131PheGly: 2.131 ± 0.315
0.639PheHis: 0.639 ± 0.177
2.184PheIle: 2.184 ± 0.385
1.705PheLys: 1.705 ± 0.281
2.237PheLeu: 2.237 ± 0.337
1.225PheMet: 1.225 ± 0.253
1.811PheAsn: 1.811 ± 0.373
1.545PhePro: 1.545 ± 0.271
0.693PheGln: 0.693 ± 0.177
2.983PheArg: 2.983 ± 0.463
3.09PheSer: 3.09 ± 0.507
2.078PheThr: 2.078 ± 0.278
2.664PheVal: 2.664 ± 0.416
0.639PheTrp: 0.639 ± 0.206
1.012PheTyr: 1.012 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
6.233GlyAla: 6.233 ± 0.905
0.586GlyCys: 0.586 ± 0.214
4.475GlyAsp: 4.475 ± 0.732
6.126GlyGlu: 6.126 ± 1.254
3.196GlyPhe: 3.196 ± 0.498
5.221GlyGly: 5.221 ± 0.716
1.225GlyHis: 1.225 ± 0.31
4.049GlyIle: 4.049 ± 0.505
5.487GlyLys: 5.487 ± 0.837
5.327GlyLeu: 5.327 ± 0.481
2.344GlyMet: 2.344 ± 0.307
2.823GlyAsn: 2.823 ± 0.348
3.942GlyPro: 3.942 ± 2.054
2.877GlyGln: 2.877 ± 0.489
4.741GlyArg: 4.741 ± 0.529
4.049GlySer: 4.049 ± 0.565
3.516GlyThr: 3.516 ± 0.414
5.061GlyVal: 5.061 ± 0.575
1.119GlyTrp: 1.119 ± 0.223
2.184GlyTyr: 2.184 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
1.918HisAla: 1.918 ± 0.237
0.053HisCys: 0.053 ± 0.053
0.959HisAsp: 0.959 ± 0.217
0.799HisGlu: 0.799 ± 0.251
0.746HisPhe: 0.746 ± 0.237
1.545HisGly: 1.545 ± 0.325
0.32HisHis: 0.32 ± 0.122
0.799HisIle: 0.799 ± 0.193
0.906HisLys: 0.906 ± 0.229
2.184HisLeu: 2.184 ± 0.518
0.479HisMet: 0.479 ± 0.201
0.639HisAsn: 0.639 ± 0.174
0.906HisPro: 0.906 ± 0.219
0.533HisGln: 0.533 ± 0.196
0.852HisArg: 0.852 ± 0.244
1.065HisSer: 1.065 ± 0.219
1.012HisThr: 1.012 ± 0.195
1.065HisVal: 1.065 ± 0.197
0.213HisTrp: 0.213 ± 0.131
0.746HisTyr: 0.746 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.167IleAla: 5.167 ± 0.452
0.693IleCys: 0.693 ± 0.167
3.409IleAsp: 3.409 ± 0.493
3.143IleGlu: 3.143 ± 0.453
1.225IlePhe: 1.225 ± 0.301
2.823IleGly: 2.823 ± 0.453
0.959IleHis: 0.959 ± 0.205
2.45IleIle: 2.45 ± 0.413
2.61IleLys: 2.61 ± 0.322
3.036IleLeu: 3.036 ± 0.539
1.119IleMet: 1.119 ± 0.217
2.823IleAsn: 2.823 ± 0.464
2.717IlePro: 2.717 ± 0.379
2.078IleGln: 2.078 ± 0.289
4.635IleArg: 4.635 ± 0.452
3.835IleSer: 3.835 ± 0.424
3.463IleThr: 3.463 ± 0.443
2.184IleVal: 2.184 ± 0.384
0.426IleTrp: 0.426 ± 0.173
1.119IleTyr: 1.119 ± 0.326
0.0IleXaa: 0.0 ± 0.0
Lys
6.499LysAla: 6.499 ± 0.543
0.586LysCys: 0.586 ± 0.189
3.303LysAsp: 3.303 ± 0.373
3.622LysGlu: 3.622 ± 0.371
1.651LysPhe: 1.651 ± 0.345
5.434LysGly: 5.434 ± 1.143
1.119LysHis: 1.119 ± 0.254
3.356LysIle: 3.356 ± 0.445
3.835LysLys: 3.835 ± 0.529
4.581LysLeu: 4.581 ± 0.503
1.492LysMet: 1.492 ± 0.293
2.983LysAsn: 2.983 ± 0.37
3.196LysPro: 3.196 ± 0.499
3.143LysGln: 3.143 ± 0.501
2.45LysArg: 2.45 ± 0.308
2.93LysSer: 2.93 ± 0.355
3.463LysThr: 3.463 ± 0.422
3.036LysVal: 3.036 ± 0.388
0.586LysTrp: 0.586 ± 0.168
1.758LysTyr: 1.758 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
7.884LeuAla: 7.884 ± 0.698
1.065LeuCys: 1.065 ± 0.286
3.835LeuAsp: 3.835 ± 0.582
4.315LeuGlu: 4.315 ± 0.516
2.877LeuPhe: 2.877 ± 0.464
4.901LeuGly: 4.901 ± 0.493
1.545LeuHis: 1.545 ± 0.285
3.25LeuIle: 3.25 ± 0.467
4.421LeuLys: 4.421 ± 0.501
6.392LeuLeu: 6.392 ± 0.692
2.237LeuMet: 2.237 ± 0.419
4.368LeuAsn: 4.368 ± 0.478
3.889LeuPro: 3.889 ± 0.473
3.942LeuGln: 3.942 ± 0.7
5.114LeuArg: 5.114 ± 0.588
5.7LeuSer: 5.7 ± 0.528
5.54LeuThr: 5.54 ± 0.56
4.741LeuVal: 4.741 ± 0.436
0.479LeuTrp: 0.479 ± 0.196
2.344LeuTyr: 2.344 ± 0.458
0.0LeuXaa: 0.0 ± 0.0
Met
2.823MetAla: 2.823 ± 0.385
0.053MetCys: 0.053 ± 0.051
1.598MetAsp: 1.598 ± 0.236
1.438MetGlu: 1.438 ± 0.255
0.693MetPhe: 0.693 ± 0.169
1.864MetGly: 1.864 ± 0.301
0.426MetHis: 0.426 ± 0.108
1.012MetIle: 1.012 ± 0.214
1.758MetLys: 1.758 ± 0.336
2.131MetLeu: 2.131 ± 0.313
0.746MetMet: 0.746 ± 0.214
1.651MetAsn: 1.651 ± 0.325
1.811MetPro: 1.811 ± 0.306
1.119MetGln: 1.119 ± 0.215
1.492MetArg: 1.492 ± 0.293
2.131MetSer: 2.131 ± 0.317
2.717MetThr: 2.717 ± 0.377
1.332MetVal: 1.332 ± 0.318
0.32MetTrp: 0.32 ± 0.15
0.746MetTyr: 0.746 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
5.061AsnAla: 5.061 ± 0.519
0.586AsnCys: 0.586 ± 0.188
1.918AsnAsp: 1.918 ± 0.344
2.983AsnGlu: 2.983 ± 0.453
1.225AsnPhe: 1.225 ± 0.287
3.463AsnGly: 3.463 ± 0.297
1.172AsnHis: 1.172 ± 0.261
2.344AsnIle: 2.344 ± 0.424
2.504AsnLys: 2.504 ± 0.381
3.25AsnLeu: 3.25 ± 0.326
1.225AsnMet: 1.225 ± 0.236
2.237AsnAsn: 2.237 ± 0.454
2.131AsnPro: 2.131 ± 0.303
1.811AsnGln: 1.811 ± 0.27
2.504AsnArg: 2.504 ± 0.442
2.184AsnSer: 2.184 ± 0.338
1.971AsnThr: 1.971 ± 0.354
2.557AsnVal: 2.557 ± 0.398
0.586AsnTrp: 0.586 ± 0.185
1.172AsnTyr: 1.172 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
3.516ProAla: 3.516 ± 0.603
0.426ProCys: 0.426 ± 0.173
4.155ProAsp: 4.155 ± 0.572
5.061ProGlu: 5.061 ± 0.884
1.598ProPhe: 1.598 ± 0.294
3.356ProGly: 3.356 ± 0.479
0.479ProHis: 0.479 ± 0.171
1.172ProIle: 1.172 ± 0.262
2.557ProLys: 2.557 ± 0.681
2.983ProLeu: 2.983 ± 0.329
0.746ProMet: 0.746 ± 0.171
1.119ProAsn: 1.119 ± 0.258
1.278ProPro: 1.278 ± 0.29
1.971ProGln: 1.971 ± 0.559
2.557ProArg: 2.557 ± 0.405
2.504ProSer: 2.504 ± 0.372
2.504ProThr: 2.504 ± 0.345
5.007ProVal: 5.007 ± 0.535
0.639ProTrp: 0.639 ± 0.184
1.811ProTyr: 1.811 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
4.208GlnAla: 4.208 ± 0.506
0.906GlnCys: 0.906 ± 0.251
2.024GlnAsp: 2.024 ± 0.316
2.93GlnGlu: 2.93 ± 0.431
1.172GlnPhe: 1.172 ± 0.215
3.463GlnGly: 3.463 ± 0.719
0.852GlnHis: 0.852 ± 0.218
2.45GlnIle: 2.45 ± 0.465
2.983GlnLys: 2.983 ± 0.504
3.995GlnLeu: 3.995 ± 0.434
0.906GlnMet: 0.906 ± 0.251
1.811GlnAsn: 1.811 ± 0.388
2.184GlnPro: 2.184 ± 0.386
3.356GlnGln: 3.356 ± 0.941
2.504GlnArg: 2.504 ± 0.576
1.971GlnSer: 1.971 ± 0.321
1.651GlnThr: 1.651 ± 0.277
2.557GlnVal: 2.557 ± 0.525
0.746GlnTrp: 0.746 ± 0.184
1.225GlnTyr: 1.225 ± 0.304
0.0GlnXaa: 0.0 ± 0.0
Arg
4.421ArgAla: 4.421 ± 0.448
0.373ArgCys: 0.373 ± 0.154
4.049ArgAsp: 4.049 ± 0.548
5.221ArgGlu: 5.221 ± 0.655
3.356ArgPhe: 3.356 ± 0.442
4.688ArgGly: 4.688 ± 0.833
1.278ArgHis: 1.278 ± 0.256
3.303ArgIle: 3.303 ± 0.468
4.368ArgLys: 4.368 ± 0.494
5.061ArgLeu: 5.061 ± 0.422
1.545ArgMet: 1.545 ± 0.265
2.983ArgAsn: 2.983 ± 0.31
2.131ArgPro: 2.131 ± 0.377
2.877ArgGln: 2.877 ± 0.419
5.38ArgArg: 5.38 ± 0.718
3.889ArgSer: 3.889 ± 0.503
3.463ArgThr: 3.463 ± 0.443
3.729ArgVal: 3.729 ± 0.473
1.332ArgTrp: 1.332 ± 0.217
2.237ArgTyr: 2.237 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
5.647SerAla: 5.647 ± 0.636
0.959SerCys: 0.959 ± 0.267
4.262SerAsp: 4.262 ± 0.497
3.942SerGlu: 3.942 ± 0.377
1.971SerPhe: 1.971 ± 0.337
5.7SerGly: 5.7 ± 0.574
0.959SerHis: 0.959 ± 0.23
2.237SerIle: 2.237 ± 0.331
2.717SerLys: 2.717 ± 0.308
5.167SerLeu: 5.167 ± 0.469
1.811SerMet: 1.811 ± 0.383
2.237SerAsn: 2.237 ± 0.329
3.196SerPro: 3.196 ± 0.373
2.504SerGln: 2.504 ± 0.324
4.208SerArg: 4.208 ± 0.444
2.77SerSer: 2.77 ± 0.398
3.569SerThr: 3.569 ± 0.374
3.835SerVal: 3.835 ± 0.469
0.746SerTrp: 0.746 ± 0.185
1.438SerTyr: 1.438 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
5.274ThrAla: 5.274 ± 0.437
0.32ThrCys: 0.32 ± 0.154
3.25ThrAsp: 3.25 ± 0.42
4.102ThrGlu: 4.102 ± 0.393
1.864ThrPhe: 1.864 ± 0.381
5.753ThrGly: 5.753 ± 0.747
1.012ThrHis: 1.012 ± 0.245
3.729ThrIle: 3.729 ± 0.496
2.664ThrLys: 2.664 ± 0.322
5.38ThrLeu: 5.38 ± 0.541
1.332ThrMet: 1.332 ± 0.282
1.545ThrAsn: 1.545 ± 0.263
3.729ThrPro: 3.729 ± 0.361
1.971ThrGln: 1.971 ± 0.379
2.557ThrArg: 2.557 ± 0.435
3.622ThrSer: 3.622 ± 0.51
3.622ThrThr: 3.622 ± 0.477
3.995ThrVal: 3.995 ± 0.429
0.959ThrTrp: 0.959 ± 0.24
1.385ThrTyr: 1.385 ± 0.295
0.0ThrXaa: 0.0 ± 0.0
Val
6.606ValAla: 6.606 ± 0.542
1.012ValCys: 1.012 ± 0.294
3.889ValAsp: 3.889 ± 0.494
3.463ValGlu: 3.463 ± 0.344
2.131ValPhe: 2.131 ± 0.329
3.09ValGly: 3.09 ± 0.538
0.852ValHis: 0.852 ± 0.233
3.463ValIle: 3.463 ± 0.497
4.049ValLys: 4.049 ± 0.492
5.7ValLeu: 5.7 ± 0.672
2.344ValMet: 2.344 ± 0.289
3.25ValAsn: 3.25 ± 0.426
2.717ValPro: 2.717 ± 0.38
2.397ValGln: 2.397 ± 0.443
4.635ValArg: 4.635 ± 0.695
4.528ValSer: 4.528 ± 0.473
4.794ValThr: 4.794 ± 0.439
4.368ValVal: 4.368 ± 0.439
0.746ValTrp: 0.746 ± 0.185
2.024ValTyr: 2.024 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
0.746TrpAla: 0.746 ± 0.217
0.213TrpCys: 0.213 ± 0.105
0.639TrpAsp: 0.639 ± 0.219
0.799TrpGlu: 0.799 ± 0.173
0.639TrpPhe: 0.639 ± 0.158
1.119TrpGly: 1.119 ± 0.334
0.373TrpHis: 0.373 ± 0.138
0.852TrpIle: 0.852 ± 0.206
0.852TrpLys: 0.852 ± 0.2
1.385TrpLeu: 1.385 ± 0.301
0.586TrpMet: 0.586 ± 0.147
0.693TrpAsn: 0.693 ± 0.222
0.693TrpPro: 0.693 ± 0.197
0.799TrpGln: 0.799 ± 0.188
1.172TrpArg: 1.172 ± 0.255
0.799TrpSer: 0.799 ± 0.19
0.426TrpThr: 0.426 ± 0.167
0.852TrpVal: 0.852 ± 0.21
0.479TrpTrp: 0.479 ± 0.155
0.213TrpTyr: 0.213 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.823TyrAla: 2.823 ± 0.464
0.213TyrCys: 0.213 ± 0.116
1.651TyrAsp: 1.651 ± 0.339
1.332TyrGlu: 1.332 ± 0.34
1.065TyrPhe: 1.065 ± 0.222
2.504TyrGly: 2.504 ± 0.456
0.426TyrHis: 0.426 ± 0.177
1.758TyrIle: 1.758 ± 0.336
0.852TyrLys: 0.852 ± 0.204
2.024TyrLeu: 2.024 ± 0.4
0.906TyrMet: 0.906 ± 0.242
1.598TyrAsn: 1.598 ± 0.278
1.278TyrPro: 1.278 ± 0.306
1.492TyrGln: 1.492 ± 0.25
2.291TyrArg: 2.291 ± 0.434
1.705TyrSer: 1.705 ± 0.238
1.598TyrThr: 1.598 ± 0.437
1.705TyrVal: 1.705 ± 0.277
0.799TyrTrp: 0.799 ± 0.191
1.065TyrTyr: 1.065 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (18773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski