Parry Creek virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Parry Creek hapavirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1I7|A0A0D3R1I7_9RHAB Phosphoprotein OS=Parry Creek virus OX=318845 PE=4 SV=1
MM1 pKa = 7.26ATNEE5 pKa = 4.11NFDD8 pKa = 4.19SKK10 pKa = 10.83LAWVVEE16 pKa = 4.18SLEE19 pKa = 4.02FHH21 pKa = 6.99PNPRR25 pKa = 11.84DD26 pKa = 3.55DD27 pKa = 4.0PVNFVISARR36 pKa = 11.84IDD38 pKa = 3.22VEE40 pKa = 4.47FPSNFDD46 pKa = 3.42EE47 pKa = 5.33VEE49 pKa = 4.04LLMHH53 pKa = 7.15ILQNLKK59 pKa = 10.06RR60 pKa = 11.84NKK62 pKa = 8.6MWPQKK67 pKa = 10.9GSFLGICAGLSLSHH81 pKa = 6.72SSFVPSDD88 pKa = 3.7PLKK91 pKa = 10.8KK92 pKa = 10.24RR93 pKa = 11.84LIGDD97 pKa = 3.54FMGVVNIPLVPSVGNDD113 pKa = 3.27YY114 pKa = 10.87IILNTTSYY122 pKa = 11.69NLDD125 pKa = 3.53LEE127 pKa = 4.51SWSEE131 pKa = 3.84IKK133 pKa = 9.48LTYY136 pKa = 10.88NFFICRR142 pKa = 11.84GNGNVTKK149 pKa = 10.56RR150 pKa = 11.84IDD152 pKa = 3.55TAWYY156 pKa = 9.0GGQPKK161 pKa = 10.08RR162 pKa = 11.84GEE164 pKa = 4.49DD165 pKa = 3.49FTFDD169 pKa = 3.94LLTVAVLYY177 pKa = 11.01GFDD180 pKa = 4.23DD181 pKa = 4.17WFASPLIDD189 pKa = 5.41RR190 pKa = 11.84DD191 pKa = 3.92DD192 pKa = 3.44

Molecular weight: 21.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1J0|A0A0D3R1J0_9RHAB Uncharacterized protein OS=Parry Creek virus OX=318845 PE=4 SV=1
MM1 pKa = 7.38GFSINFDD8 pKa = 4.02PIINKK13 pKa = 8.68FRR15 pKa = 11.84EE16 pKa = 4.06FQTNINNNINEE27 pKa = 4.17QLDD30 pKa = 3.8KK31 pKa = 11.25IKK33 pKa = 10.54IIWANLGTHH42 pKa = 5.68IKK44 pKa = 10.05YY45 pKa = 9.57WFILIISILIILAVLFLLIKK65 pKa = 9.17ITRR68 pKa = 11.84LILNCKK74 pKa = 10.05KK75 pKa = 10.28IFSCCCSWCCKK86 pKa = 8.71KK87 pKa = 10.73QKK89 pKa = 6.51TQRR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 9.82DD95 pKa = 3.62DD96 pKa = 3.33KK97 pKa = 11.62VKK99 pKa = 10.53IFSITPP105 pKa = 3.42

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4250

105

2118

472.2

54.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.012 ± 0.236

1.906 ± 0.192

6.071 ± 0.628

5.953 ± 0.422

5.059 ± 0.318

5.388 ± 0.493

2.659 ± 0.349

8.4 ± 0.702

7.435 ± 0.302

10.024 ± 0.624

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.187

6.235 ± 0.385

4.376 ± 0.333

2.988 ± 0.338

4.094 ± 0.2

8.165 ± 0.361

4.894 ± 0.427

4.776 ± 0.36

2.259 ± 0.168

3.906 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski