Thiomicrospira sp. WB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Thiomicrospira; unclassified Thiomicrospira

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2043 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X3TB83|A0A0X3TB83_9GAMM Allophanate hydrolase OS=Thiomicrospira sp. WB1 OX=1685380 GN=AVO41_03825 PE=4 SV=1
MM1 pKa = 8.99AMMLLILVVTPFFYY15 pKa = 10.41QAAMQTANEE24 pKa = 4.14MQRR27 pKa = 11.84DD28 pKa = 3.86QDD30 pKa = 3.46QKK32 pKa = 9.68IQQRR36 pKa = 11.84LEE38 pKa = 3.95DD39 pKa = 4.48LKK41 pKa = 11.47DD42 pKa = 4.08LIILFSKK49 pKa = 10.75YY50 pKa = 9.99PEE52 pKa = 4.44LFCAGADD59 pKa = 3.65NEE61 pKa = 4.36SDD63 pKa = 3.02AGYY66 pKa = 11.37LMVPDD71 pKa = 3.72AAGYY75 pKa = 8.51TEE77 pKa = 5.24VEE79 pKa = 4.31FQAFKK84 pKa = 10.35PYY86 pKa = 10.48EE87 pKa = 4.14NQIHH91 pKa = 5.86IQPLIDD97 pKa = 4.6NDD99 pKa = 3.73NDD101 pKa = 3.76PANANNYY108 pKa = 8.69VVGSCSDD115 pKa = 3.28VTTPSPGVEE124 pKa = 4.27KK125 pKa = 10.81ACDD128 pKa = 3.2VATDD132 pKa = 3.65QNLLLEE138 pKa = 4.81ISWQGDD144 pKa = 3.79SAGLPVINASTPVVYY159 pKa = 10.24LYY161 pKa = 10.57RR162 pKa = 11.84DD163 pKa = 3.4EE164 pKa = 5.43LGCC167 pKa = 5.15

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X3T7P2|A0A0X3T7P2_9GAMM MPT synthase subunit 2 OS=Thiomicrospira sp. WB1 OX=1685380 GN=AVO41_10265 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.27RR3 pKa = 11.84LSGDD7 pKa = 3.43LGRR10 pKa = 11.84RR11 pKa = 11.84WWLVALGLVLGLNSAIAGPPMAAFDD36 pKa = 4.29SPSQYY41 pKa = 10.41EE42 pKa = 4.18QYY44 pKa = 11.01QKK46 pKa = 10.04LTEE49 pKa = 3.94HH50 pKa = 6.67LRR52 pKa = 11.84CVVCANQSIQASQAPVAKK70 pKa = 10.49AMRR73 pKa = 11.84NDD75 pKa = 3.09VYY77 pKa = 11.25QRR79 pKa = 11.84LVKK82 pKa = 10.21KK83 pKa = 10.24AQRR86 pKa = 11.84PEE88 pKa = 3.8QIIAAMQRR96 pKa = 11.84EE97 pKa = 4.74YY98 pKa = 11.26GDD100 pKa = 3.49QVHH103 pKa = 5.89YY104 pKa = 9.99RR105 pKa = 11.84PPMRR109 pKa = 11.84GAHH112 pKa = 4.76WVLWLGPFVLLLLAVGMGGFWLTRR136 pKa = 11.84ARR138 pKa = 11.84HH139 pKa = 4.79RR140 pKa = 11.84HH141 pKa = 5.27ASQTPNQKK149 pKa = 8.44GTSLL153 pKa = 3.49

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2043

0

2043

688134

41

2012

336.8

37.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.603 ± 0.056

0.797 ± 0.017

5.884 ± 0.045

6.287 ± 0.048

3.823 ± 0.032

6.87 ± 0.054

2.534 ± 0.032

5.156 ± 0.044

4.46 ± 0.043

10.674 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.026

3.381 ± 0.041

4.536 ± 0.035

5.576 ± 0.056

5.367 ± 0.043

5.739 ± 0.036

5.451 ± 0.036

6.921 ± 0.047

1.487 ± 0.028

2.731 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski