Sweet potato leaf curl Hubei virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A345GSG2|A0A345GSG2_9GEMI Protein V2 OS=Sweet potato leaf curl Hubei virus OX=2282485 PE=3 SV=1
MM1 pKa = 7.17EE2 pKa = 4.15QSLWDD7 pKa = 4.38PLTHH11 pKa = 6.93PLPEE15 pKa = 4.21TLHH18 pKa = 6.51GFRR21 pKa = 11.84CMLSLKK27 pKa = 10.18YY28 pKa = 10.11MSNIRR33 pKa = 11.84DD34 pKa = 3.47KK35 pKa = 11.56YY36 pKa = 9.73EE37 pKa = 4.39PGTLGHH43 pKa = 6.61EE44 pKa = 4.23LAVLLIRR51 pKa = 11.84SLRR54 pKa = 11.84GKK56 pKa = 10.16NYY58 pKa = 9.19VRR60 pKa = 11.84STSSYY65 pKa = 10.99EE66 pKa = 3.99EE67 pKa = 4.04VCALLSEE74 pKa = 4.68TAGSSPSKK82 pKa = 10.03LHH84 pKa = 6.56DD85 pKa = 3.16RR86 pKa = 11.84HH87 pKa = 6.5RR88 pKa = 11.84PVCGEE93 pKa = 4.33CCPACCSNVRR103 pKa = 11.84SSSCEE108 pKa = 3.54GKK110 pKa = 10.29EE111 pKa = 3.64ADD113 pKa = 3.54LSKK116 pKa = 10.75EE117 pKa = 3.77RR118 pKa = 11.84RR119 pKa = 11.84LDD121 pKa = 3.48SS122 pKa = 3.79

Molecular weight:
13.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A345GSF9|A0A345GSF9_9GEMI AC4 OS=Sweet potato leaf curl Hubei virus OX=2282485 PE=3 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.26RR4 pKa = 11.84LRR6 pKa = 11.84VTRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 10.01APYY14 pKa = 10.32SRR16 pKa = 11.84RR17 pKa = 11.84PQAARR22 pKa = 11.84RR23 pKa = 11.84LNFTTDD29 pKa = 2.51IVPYY33 pKa = 9.65VGNAAPLAAATYY45 pKa = 10.31VPVPVKK51 pKa = 10.37ARR53 pKa = 11.84KK54 pKa = 7.52RR55 pKa = 11.84TSRR58 pKa = 11.84KK59 pKa = 9.25RR60 pKa = 11.84GDD62 pKa = 3.66WIPRR66 pKa = 11.84GCVGPCKK73 pKa = 10.08VQDD76 pKa = 3.86YY77 pKa = 8.72EE78 pKa = 4.55FKK80 pKa = 10.3MDD82 pKa = 3.6VPHH85 pKa = 7.17GGTFVCVSDD94 pKa = 3.96FTRR97 pKa = 11.84GTGLTHH103 pKa = 7.28RR104 pKa = 11.84LGKK107 pKa = 9.86RR108 pKa = 11.84VCIKK112 pKa = 11.1SMGFSGKK119 pKa = 7.94VWMDD123 pKa = 3.53DD124 pKa = 3.61NIAKK128 pKa = 9.84KK129 pKa = 10.52DD130 pKa = 3.43HH131 pKa = 6.37TNIITFWLVRR141 pKa = 11.84DD142 pKa = 3.76RR143 pKa = 11.84RR144 pKa = 11.84PNKK147 pKa = 10.42DD148 pKa = 3.21PLTFSQLFHH157 pKa = 6.56MFDD160 pKa = 4.05NEE162 pKa = 3.9PLTAKK167 pKa = 10.05VRR169 pKa = 11.84TDD171 pKa = 2.9LRR173 pKa = 11.84DD174 pKa = 3.17RR175 pKa = 11.84FQVLRR180 pKa = 11.84TFSVTVSGGPYY191 pKa = 9.23AHH193 pKa = 7.66KK194 pKa = 10.09EE195 pKa = 3.71QAQVRR200 pKa = 11.84RR201 pKa = 11.84FFKK204 pKa = 10.71GLNNHH209 pKa = 6.26VIYY212 pKa = 10.49NHH214 pKa = 6.53KK215 pKa = 10.73EE216 pKa = 3.41EE217 pKa = 4.8AKK219 pKa = 10.88YY220 pKa = 10.75EE221 pKa = 4.12NQLEE225 pKa = 4.18NAMLVYY231 pKa = 10.27SASSHH236 pKa = 6.1ASNPVYY242 pKa = 10.32QSLRR246 pKa = 11.84VRR248 pKa = 11.84AYY250 pKa = 10.48FYY252 pKa = 10.58DD253 pKa = 3.16SHH255 pKa = 6.48MNN257 pKa = 3.42

Molecular weight:
29.7 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1165

122

360

194.2

22.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.408 ± 0.805

2.661 ± 0.861

4.807 ± 0.412

4.979 ± 1.091

4.206 ± 0.581

5.236 ± 0.278

3.691 ± 0.346

5.15 ± 1.054

5.923 ± 0.395

8.24 ± 1.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.608

4.549 ± 0.883

6.438 ± 0.748

4.378 ± 0.66

6.867 ± 1.138

9.871 ± 1.614

5.408 ± 0.851

5.15 ± 1.032

1.459 ± 0.214

3.605 ± 0.49

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski