Drosophila melanogaster sigmavirus HAP23

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Drosophila melanogaster sigmavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8CAQ0|C8CAQ0_9RHAB Uncharacterized protein X OS=Drosophila melanogaster sigmavirus HAP23 OX=666961 GN=X PE=4 SV=1
MM1 pKa = 7.54NLTQEE6 pKa = 4.2DD7 pKa = 4.14EE8 pKa = 4.22RR9 pKa = 11.84RR10 pKa = 11.84LALLRR15 pKa = 11.84EE16 pKa = 4.19RR17 pKa = 11.84AAAWDD22 pKa = 3.5HH23 pKa = 6.62ANALGEE29 pKa = 4.22FEE31 pKa = 5.82SNAEE35 pKa = 3.65LDD37 pKa = 3.97VYY39 pKa = 10.99HH40 pKa = 7.07EE41 pKa = 5.32GIAYY45 pKa = 7.94PPSIDD50 pKa = 3.37EE51 pKa = 4.39MEE53 pKa = 4.31VGEE56 pKa = 5.75DD57 pKa = 4.17RR58 pKa = 11.84PTLDD62 pKa = 5.05KK63 pKa = 10.87ILEE66 pKa = 4.08SSIGSEE72 pKa = 3.97SLPNLEE78 pKa = 5.59DD79 pKa = 4.15GPPDD83 pKa = 3.29QEE85 pKa = 4.05AAYY88 pKa = 10.18HH89 pKa = 5.52EE90 pKa = 4.71GEE92 pKa = 4.67MYY94 pKa = 9.8STMTTSYY101 pKa = 9.49MDD103 pKa = 3.34QGNEE107 pKa = 4.46SMDD110 pKa = 3.68LSAGPTCSTPQPITGIHH127 pKa = 6.11HH128 pKa = 6.96KK129 pKa = 8.77EE130 pKa = 4.18TINGRR135 pKa = 11.84VTQMVYY141 pKa = 10.01IGEE144 pKa = 4.29TSDD147 pKa = 3.03MALINRR153 pKa = 11.84LQDD156 pKa = 3.57EE157 pKa = 4.42FDD159 pKa = 3.48VLYY162 pKa = 11.3NMCPNRR168 pKa = 11.84NRR170 pKa = 11.84RR171 pKa = 11.84GEE173 pKa = 4.12IVHH176 pKa = 6.84LIVMWSTPLKK186 pKa = 10.1PSTRR190 pKa = 11.84YY191 pKa = 9.89SLSPAQPPGKK201 pKa = 9.49KK202 pKa = 9.58RR203 pKa = 11.84KK204 pKa = 8.95IVQHH208 pKa = 6.33SSDD211 pKa = 4.34PEE213 pKa = 3.95PEE215 pKa = 3.88IVFVPEE221 pKa = 4.2PTSSEE226 pKa = 3.6QSLPEE231 pKa = 4.01RR232 pKa = 11.84QIKK235 pKa = 10.25AYY237 pKa = 8.41LQRR240 pKa = 11.84GIEE243 pKa = 4.16LKK245 pKa = 10.67GKK247 pKa = 9.54SAEE250 pKa = 4.11VPNFKK255 pKa = 8.75ITNEE259 pKa = 4.01TLGFSDD265 pKa = 5.4ANLKK269 pKa = 10.15LLYY272 pKa = 9.26PDD274 pKa = 3.44TSLYY278 pKa = 9.8PDD280 pKa = 3.49NPVFILEE287 pKa = 4.53DD288 pKa = 3.56VFTSTRR294 pKa = 11.84QLNKK298 pKa = 10.16IKK300 pKa = 10.8VNYY303 pKa = 9.18QLYY306 pKa = 9.82EE307 pKa = 3.92PVLPSII313 pKa = 4.87

Molecular weight: 35.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8CAQ2|C8CAQ2_9RHAB Glycoprotein OS=Drosophila melanogaster sigmavirus HAP23 OX=666961 GN=G PE=4 SV=1
MM1 pKa = 7.43NKK3 pKa = 9.12MNQLVRR9 pKa = 11.84FVKK12 pKa = 10.03DD13 pKa = 3.36TVAVRR18 pKa = 11.84KK19 pKa = 8.27PQSEE23 pKa = 4.25DD24 pKa = 3.1QLYY27 pKa = 10.81LPIPSTIGGHH37 pKa = 5.33EE38 pKa = 4.04VDD40 pKa = 4.88SPFAEE45 pKa = 4.34PTAPTLGIIQSKK57 pKa = 9.57CKK59 pKa = 10.16RR60 pKa = 11.84ADD62 pKa = 3.27WLIKK66 pKa = 10.12SHH68 pKa = 6.41LTITTNYY75 pKa = 9.13EE76 pKa = 3.56IKK78 pKa = 10.27EE79 pKa = 4.06WEE81 pKa = 4.01TWDD84 pKa = 4.09RR85 pKa = 11.84AISDD89 pKa = 3.89ILDD92 pKa = 4.21LYY94 pKa = 11.07DD95 pKa = 4.0GNPVFKK101 pKa = 10.42PILLFVYY108 pKa = 10.2YY109 pKa = 10.35VLAYY113 pKa = 9.41NARR116 pKa = 11.84KK117 pKa = 9.69IPGPFNGVRR126 pKa = 11.84YY127 pKa = 8.89GAYY130 pKa = 9.82FDD132 pKa = 4.64EE133 pKa = 5.29LTTVWHH139 pKa = 7.39AIPEE143 pKa = 4.28LMNQEE148 pKa = 3.24TDD150 pKa = 2.81YY151 pKa = 11.23SYY153 pKa = 10.95NHH155 pKa = 6.5RR156 pKa = 11.84VIHH159 pKa = 6.69RR160 pKa = 11.84KK161 pKa = 7.12IQYY164 pKa = 9.38VISFKK169 pKa = 10.67IQMSSTKK176 pKa = 10.13RR177 pKa = 11.84RR178 pKa = 11.84TSPIEE183 pKa = 3.66SFIEE187 pKa = 4.23VTSEE191 pKa = 4.07GLKK194 pKa = 8.66HH195 pKa = 5.87TPQFTTILDD204 pKa = 3.58RR205 pKa = 11.84ARR207 pKa = 11.84FVYY210 pKa = 10.56SLTGGRR216 pKa = 11.84YY217 pKa = 9.15VIHH220 pKa = 7.08PFF222 pKa = 3.4

Molecular weight: 25.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3973

222

2129

662.2

75.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.48 ± 0.277

1.46 ± 0.2

5.512 ± 0.299

5.663 ± 0.623

4.027 ± 0.319

5.99 ± 0.394

2.794 ± 0.289

6.871 ± 0.215

4.631 ± 0.404

9.665 ± 0.487

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.176

4.531 ± 0.197

5.361 ± 0.439

3.599 ± 0.062

5.512 ± 0.442

8.256 ± 0.349

6.947 ± 0.357

6.393 ± 0.527

1.611 ± 0.289

3.927 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski