Escherichia virus CBA120

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Cvivirinae; Kuttervirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3M1B7|G3M1B7_9CAUD Conserved hypothetical phage protein OS=Escherichia virus CBA120 OX=1987159 GN=orf243 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.23LNKK5 pKa = 9.49ILLVCALAFSTTACSTLLDD24 pKa = 3.75VASTVDD30 pKa = 3.92LDD32 pKa = 4.74APTFTNQQAVNKK44 pKa = 9.85MEE46 pKa = 4.2DD47 pKa = 3.51TIKK50 pKa = 11.04AHH52 pKa = 6.87AALDD56 pKa = 3.77NTTPGPLQTVCNYY69 pKa = 10.58DD70 pKa = 3.97DD71 pKa = 5.54SIQEE75 pKa = 4.24DD76 pKa = 3.93EE77 pKa = 4.95TYY79 pKa = 10.8HH80 pKa = 5.37CTTYY84 pKa = 10.81VKK86 pKa = 10.2EE87 pKa = 4.33SSVVLYY93 pKa = 10.72ADD95 pKa = 3.77CTEE98 pKa = 4.32EE99 pKa = 3.89QCTATGYY106 pKa = 10.78DD107 pKa = 3.34QVEE110 pKa = 4.46KK111 pKa = 10.86SDD113 pKa = 3.61EE114 pKa = 3.95

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3M166|G3M166_9CAUD Uncharacterized protein OS=Escherichia virus CBA120 OX=1987159 GN=orf179 PE=4 SV=1
MM1 pKa = 7.37ACKK4 pKa = 9.41TPEE7 pKa = 3.85MKK9 pKa = 10.54LVVSSTGRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84SRR22 pKa = 11.84SRR24 pKa = 11.84NLITRR29 pKa = 11.84LDD31 pKa = 3.63ILRR34 pKa = 11.84QKK36 pKa = 9.72YY37 pKa = 9.03QGHH40 pKa = 5.49SVFTRR45 pKa = 11.84IRR47 pKa = 11.84NALKK51 pKa = 10.19EE52 pKa = 3.98GRR54 pKa = 11.84TEE56 pKa = 4.12LEE58 pKa = 3.91LYY60 pKa = 10.03RR61 pKa = 11.84PNGSTRR67 pKa = 11.84AYY69 pKa = 8.21QTTDD73 pKa = 2.69GLLEE77 pKa = 4.85LIRR80 pKa = 11.84LSGMSIEE87 pKa = 4.55PRR89 pKa = 11.84SSGTPLCSLYY99 pKa = 11.17VIGNLGALL107 pKa = 3.84

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

203

1

204

47877

42

1612

234.7

26.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.573 ± 0.155

1.168 ± 0.072

6.448 ± 0.1

6.508 ± 0.19

4.342 ± 0.102

6.73 ± 0.191

1.876 ± 0.104

6.36 ± 0.127

6.379 ± 0.16

7.983 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.098

5.197 ± 0.121

3.897 ± 0.112

3.833 ± 0.099

4.913 ± 0.125

6.479 ± 0.169

6.022 ± 0.176

7.145 ± 0.17

1.391 ± 0.067

3.895 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski