Noviherbaspirillum denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Noviherbaspirillum

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5003 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A254TID7|A0A254TID7_9BURK Histidine kinase OS=Noviherbaspirillum denitrificans OX=1968433 GN=AYR66_25800 PE=4 SV=1
MM1 pKa = 7.15NAVTEE6 pKa = 4.27MPDD9 pKa = 3.74PIVFTDD15 pKa = 3.86SAASKK20 pKa = 9.42VAQLIEE26 pKa = 4.38EE27 pKa = 4.51EE28 pKa = 4.63GNPDD32 pKa = 2.85LKK34 pKa = 11.13LRR36 pKa = 11.84VFVQGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.69EE55 pKa = 4.93IVNEE59 pKa = 5.02DD60 pKa = 4.04DD61 pKa = 3.2TTMTKK66 pKa = 10.66NGVQLLIDD74 pKa = 3.77SMSYY78 pKa = 10.23QYY80 pKa = 11.55LVGAEE85 pKa = 4.01IDD87 pKa = 3.89YY88 pKa = 11.28KK89 pKa = 11.39DD90 pKa = 4.47DD91 pKa = 4.08LEE93 pKa = 4.56GAQFVIKK100 pKa = 10.5NPNATTTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A254TG60|A0A254TG60_9BURK ABC transporter permease OS=Noviherbaspirillum denitrificans OX=1968433 GN=AYR66_21315 PE=4 SV=1
MM1 pKa = 7.18LHH3 pKa = 6.33FVAPHH8 pKa = 4.91RR9 pKa = 11.84TKK11 pKa = 10.69RR12 pKa = 11.84AGRR15 pKa = 11.84PRR17 pKa = 11.84NRR19 pKa = 11.84RR20 pKa = 11.84SAWAVRR26 pKa = 11.84GGLPRR31 pKa = 11.84AASLSLALAGFLLPFAAWCMVSGAGWVDD59 pKa = 3.67PVFLPTPLQVVEE71 pKa = 5.17RR72 pKa = 11.84CLDD75 pKa = 3.48WFRR78 pKa = 11.84SDD80 pKa = 5.08GLTGDD85 pKa = 3.23IGISIGRR92 pKa = 11.84VFGGFFASALVAIPLGVLIGAYY114 pKa = 10.2APVRR118 pKa = 11.84SFFEE122 pKa = 4.07PLTDD126 pKa = 4.3FSRR129 pKa = 11.84YY130 pKa = 9.06LPAVAFIPLIMLWVGIDD147 pKa = 3.56EE148 pKa = 4.33SAKK151 pKa = 10.83LSVIWIGTFFQMVLMLAEE169 pKa = 5.2DD170 pKa = 3.9IARR173 pKa = 11.84VPNAPIEE180 pKa = 4.16AARR183 pKa = 11.84TMGATNGEE191 pKa = 4.28IVKK194 pKa = 10.37HH195 pKa = 4.68VLIPAARR202 pKa = 11.84PAMLDD207 pKa = 3.39TLRR210 pKa = 11.84STMGLAWTYY219 pKa = 10.92LVVAEE224 pKa = 4.69LVAANSGLGFSILKK238 pKa = 9.68AQRR241 pKa = 11.84FLQTDD246 pKa = 4.13KK247 pKa = 11.1IFVGILLIGLIGLLTDD263 pKa = 3.42QAFRR267 pKa = 11.84WLHH270 pKa = 4.92RR271 pKa = 11.84RR272 pKa = 11.84CFAWHH277 pKa = 6.27NARR280 pKa = 11.84SS281 pKa = 3.51

Molecular weight: 30.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5003

0

5003

1573312

37

2134

314.5

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.657 ± 0.045

0.972 ± 0.011

5.377 ± 0.025

5.746 ± 0.031

3.766 ± 0.02

8.015 ± 0.035

2.258 ± 0.017

5.144 ± 0.022

4.083 ± 0.034

10.133 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.016

3.1 ± 0.021

4.792 ± 0.03

3.681 ± 0.021

6.469 ± 0.03

5.548 ± 0.023

5.245 ± 0.027

7.58 ± 0.028

1.259 ± 0.014

2.453 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski