Amino acid dipepetide frequency for Cyanophage P-SSP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.858AlaAla: 8.858 ± 0.976
0.757AlaCys: 0.757 ± 0.268
6.208AlaAsp: 6.208 ± 1.228
3.786AlaGlu: 3.786 ± 0.561
3.483AlaPhe: 3.483 ± 0.432
5.678AlaGly: 5.678 ± 0.723
1.363AlaHis: 1.363 ± 0.4
3.861AlaIle: 3.861 ± 0.466
5.527AlaLys: 5.527 ± 0.975
5.603AlaLeu: 5.603 ± 0.515
2.423AlaMet: 2.423 ± 0.453
4.694AlaAsn: 4.694 ± 0.612
2.196AlaPro: 2.196 ± 0.37
2.877AlaGln: 2.877 ± 0.641
3.71AlaArg: 3.71 ± 0.667
5.906AlaSer: 5.906 ± 0.633
5.451AlaThr: 5.451 ± 0.723
5.148AlaVal: 5.148 ± 0.563
0.757AlaTrp: 0.757 ± 0.197
2.574AlaTyr: 2.574 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.53CysAla: 0.53 ± 0.178
0.151CysCys: 0.151 ± 0.107
0.53CysAsp: 0.53 ± 0.204
0.303CysGlu: 0.303 ± 0.144
0.227CysPhe: 0.227 ± 0.125
0.454CysGly: 0.454 ± 0.152
0.227CysHis: 0.227 ± 0.161
0.303CysIle: 0.303 ± 0.144
0.757CysLys: 0.757 ± 0.214
0.757CysLeu: 0.757 ± 0.229
0.227CysMet: 0.227 ± 0.118
0.303CysAsn: 0.303 ± 0.148
0.454CysPro: 0.454 ± 0.219
0.303CysGln: 0.303 ± 0.141
0.606CysArg: 0.606 ± 0.21
0.984CysSer: 0.984 ± 0.297
1.136CysThr: 1.136 ± 0.281
0.909CysVal: 0.909 ± 0.263
0.0CysTrp: 0.0 ± 0.0
0.303CysTyr: 0.303 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
5.376AspAla: 5.376 ± 0.547
0.454AspCys: 0.454 ± 0.184
5.83AspAsp: 5.83 ± 0.792
3.937AspGlu: 3.937 ± 0.515
2.65AspPhe: 2.65 ± 0.47
5.3AspGly: 5.3 ± 0.694
0.757AspHis: 0.757 ± 0.263
4.694AspIle: 4.694 ± 0.509
3.937AspLys: 3.937 ± 0.668
6.663AspLeu: 6.663 ± 0.858
0.681AspMet: 0.681 ± 0.231
2.953AspAsn: 2.953 ± 0.481
2.877AspPro: 2.877 ± 0.701
2.574AspGln: 2.574 ± 0.522
2.65AspArg: 2.65 ± 0.438
4.164AspSer: 4.164 ± 0.726
4.846AspThr: 4.846 ± 0.566
4.088AspVal: 4.088 ± 0.662
0.984AspTrp: 0.984 ± 0.289
1.969AspTyr: 1.969 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
5.224GluAla: 5.224 ± 0.663
0.303GluCys: 0.303 ± 0.138
4.24GluAsp: 4.24 ± 0.527
5.3GluGlu: 5.3 ± 0.905
2.12GluPhe: 2.12 ± 0.444
3.861GluGly: 3.861 ± 0.492
1.363GluHis: 1.363 ± 0.393
4.391GluIle: 4.391 ± 0.766
3.558GluLys: 3.558 ± 0.632
5.754GluLeu: 5.754 ± 0.693
1.514GluMet: 1.514 ± 0.349
2.801GluAsn: 2.801 ± 0.498
2.044GluPro: 2.044 ± 0.475
2.498GluGln: 2.498 ± 0.431
3.028GluArg: 3.028 ± 0.412
3.18GluSer: 3.18 ± 0.442
4.694GluThr: 4.694 ± 0.57
3.937GluVal: 3.937 ± 0.494
0.606GluTrp: 0.606 ± 0.245
1.741GluTyr: 1.741 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.726PheAla: 2.726 ± 0.571
0.379PheCys: 0.379 ± 0.172
2.347PheAsp: 2.347 ± 0.479
1.969PheGlu: 1.969 ± 0.454
1.666PhePhe: 1.666 ± 0.437
3.028PheGly: 3.028 ± 0.507
0.454PheHis: 0.454 ± 0.201
2.498PheIle: 2.498 ± 0.382
2.801PheLys: 2.801 ± 0.533
2.801PheLeu: 2.801 ± 0.622
1.287PheMet: 1.287 ± 0.324
2.801PheAsn: 2.801 ± 0.49
1.136PhePro: 1.136 ± 0.294
1.287PheGln: 1.287 ± 0.292
1.136PheArg: 1.136 ± 0.273
2.574PheSer: 2.574 ± 0.53
2.574PheThr: 2.574 ± 0.538
3.028PheVal: 3.028 ± 0.478
0.227PheTrp: 0.227 ± 0.12
1.06PheTyr: 1.06 ± 0.249
0.0PheXaa: 0.0 ± 0.0
Gly
5.148GlyAla: 5.148 ± 0.667
0.227GlyCys: 0.227 ± 0.128
5.603GlyAsp: 5.603 ± 0.656
3.786GlyGlu: 3.786 ± 0.507
3.104GlyPhe: 3.104 ± 0.362
4.316GlyGly: 4.316 ± 0.721
0.833GlyHis: 0.833 ± 0.259
4.088GlyIle: 4.088 ± 0.739
4.316GlyLys: 4.316 ± 0.48
4.846GlyLeu: 4.846 ± 0.453
1.287GlyMet: 1.287 ± 0.308
4.391GlyAsn: 4.391 ± 0.678
1.439GlyPro: 1.439 ± 0.345
2.271GlyGln: 2.271 ± 0.435
3.18GlyArg: 3.18 ± 0.581
5.148GlySer: 5.148 ± 1.191
5.906GlyThr: 5.906 ± 0.866
5.451GlyVal: 5.451 ± 0.693
0.833GlyTrp: 0.833 ± 0.232
2.726GlyTyr: 2.726 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
0.303HisAla: 0.303 ± 0.128
0.227HisCys: 0.227 ± 0.132
1.363HisAsp: 1.363 ± 0.482
1.363HisGlu: 1.363 ± 0.344
0.606HisPhe: 0.606 ± 0.267
1.514HisGly: 1.514 ± 0.343
0.454HisHis: 0.454 ± 0.265
1.06HisIle: 1.06 ± 0.468
0.909HisLys: 0.909 ± 0.287
1.741HisLeu: 1.741 ± 0.372
0.151HisMet: 0.151 ± 0.099
0.757HisAsn: 0.757 ± 0.27
0.606HisPro: 0.606 ± 0.201
0.833HisGln: 0.833 ± 0.257
0.53HisArg: 0.53 ± 0.202
0.53HisSer: 0.53 ± 0.192
1.211HisThr: 1.211 ± 0.325
0.757HisVal: 0.757 ± 0.237
0.379HisTrp: 0.379 ± 0.179
0.984HisTyr: 0.984 ± 0.384
0.0HisXaa: 0.0 ± 0.0
Ile
5.3IleAla: 5.3 ± 1.23
0.379IleCys: 0.379 ± 0.187
4.921IleAsp: 4.921 ± 0.506
3.786IleGlu: 3.786 ± 0.633
1.741IlePhe: 1.741 ± 0.41
3.407IleGly: 3.407 ± 0.648
1.287IleHis: 1.287 ± 0.272
3.256IleIle: 3.256 ± 0.424
4.694IleLys: 4.694 ± 0.572
3.71IleLeu: 3.71 ± 0.568
1.514IleMet: 1.514 ± 0.365
3.407IleAsn: 3.407 ± 0.502
3.256IlePro: 3.256 ± 0.544
2.877IleGln: 2.877 ± 0.471
2.726IleArg: 2.726 ± 0.413
4.391IleSer: 4.391 ± 0.526
3.634IleThr: 3.634 ± 0.592
2.877IleVal: 2.877 ± 0.592
0.606IleTrp: 0.606 ± 0.238
1.514IleTyr: 1.514 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
5.3LysAla: 5.3 ± 0.799
0.454LysCys: 0.454 ± 0.222
4.618LysAsp: 4.618 ± 0.525
4.391LysGlu: 4.391 ± 0.762
3.18LysPhe: 3.18 ± 0.545
3.786LysGly: 3.786 ± 0.91
1.287LysHis: 1.287 ± 0.32
4.088LysIle: 4.088 ± 1.254
5.83LysLys: 5.83 ± 0.912
6.965LysLeu: 6.965 ± 0.625
1.363LysMet: 1.363 ± 0.439
3.104LysAsn: 3.104 ± 0.53
2.801LysPro: 2.801 ± 0.525
2.801LysGln: 2.801 ± 0.472
3.104LysArg: 3.104 ± 0.608
4.088LysSer: 4.088 ± 0.531
3.937LysThr: 3.937 ± 0.479
3.634LysVal: 3.634 ± 0.523
0.681LysTrp: 0.681 ± 0.241
2.574LysTyr: 2.574 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
5.678LeuAla: 5.678 ± 0.659
1.06LeuCys: 1.06 ± 0.267
5.83LeuAsp: 5.83 ± 0.556
4.391LeuGlu: 4.391 ± 0.751
2.347LeuPhe: 2.347 ± 0.397
5.678LeuGly: 5.678 ± 0.745
1.439LeuHis: 1.439 ± 0.396
4.997LeuIle: 4.997 ± 0.634
6.284LeuLys: 6.284 ± 0.581
6.511LeuLeu: 6.511 ± 0.598
1.741LeuMet: 1.741 ± 0.417
3.71LeuAsn: 3.71 ± 0.554
3.558LeuPro: 3.558 ± 0.542
4.316LeuGln: 4.316 ± 0.8
3.558LeuArg: 3.558 ± 0.459
4.997LeuSer: 4.997 ± 0.521
5.224LeuThr: 5.224 ± 0.61
5.148LeuVal: 5.148 ± 0.659
0.606LeuTrp: 0.606 ± 0.232
3.786LeuTyr: 3.786 ± 0.81
0.0LeuXaa: 0.0 ± 0.0
Met
2.044MetAla: 2.044 ± 0.354
0.303MetCys: 0.303 ± 0.159
1.211MetAsp: 1.211 ± 0.307
1.514MetGlu: 1.514 ± 0.385
0.757MetPhe: 0.757 ± 0.248
1.363MetGly: 1.363 ± 0.63
0.53MetHis: 0.53 ± 0.252
0.984MetIle: 0.984 ± 0.258
2.347MetLys: 2.347 ± 0.453
1.969MetLeu: 1.969 ± 0.319
0.076MetMet: 0.076 ± 0.073
1.06MetAsn: 1.06 ± 0.259
0.909MetPro: 0.909 ± 0.21
1.06MetGln: 1.06 ± 0.317
0.909MetArg: 0.909 ± 0.207
1.666MetSer: 1.666 ± 0.435
1.666MetThr: 1.666 ± 0.416
1.06MetVal: 1.06 ± 0.302
0.227MetTrp: 0.227 ± 0.094
0.833MetTyr: 0.833 ± 0.261
0.0MetXaa: 0.0 ± 0.0
Asn
5.376AsnAla: 5.376 ± 0.826
0.833AsnCys: 0.833 ± 0.283
2.196AsnAsp: 2.196 ± 0.445
2.271AsnGlu: 2.271 ± 0.337
1.969AsnPhe: 1.969 ± 0.342
5.376AsnGly: 5.376 ± 0.992
0.757AsnHis: 0.757 ± 0.198
3.256AsnIle: 3.256 ± 0.395
3.331AsnLys: 3.331 ± 0.7
4.24AsnLeu: 4.24 ± 0.554
0.757AsnMet: 0.757 ± 0.196
3.483AsnAsn: 3.483 ± 0.629
3.104AsnPro: 3.104 ± 0.555
2.12AsnGln: 2.12 ± 0.431
3.028AsnArg: 3.028 ± 0.72
4.013AsnSer: 4.013 ± 1.25
3.407AsnThr: 3.407 ± 0.531
2.953AsnVal: 2.953 ± 0.55
0.757AsnTrp: 0.757 ± 0.216
1.817AsnTyr: 1.817 ± 0.304
0.0AsnXaa: 0.0 ± 0.0
Pro
2.271ProAla: 2.271 ± 0.433
0.303ProCys: 0.303 ± 0.159
2.726ProAsp: 2.726 ± 0.471
3.937ProGlu: 3.937 ± 0.787
1.136ProPhe: 1.136 ± 0.249
2.044ProGly: 2.044 ± 0.443
0.379ProHis: 0.379 ± 0.163
2.423ProIle: 2.423 ± 0.543
1.817ProLys: 1.817 ± 0.478
2.498ProLeu: 2.498 ± 0.435
0.909ProMet: 0.909 ± 0.281
2.423ProAsn: 2.423 ± 0.316
2.196ProPro: 2.196 ± 0.789
1.969ProGln: 1.969 ± 0.389
0.909ProArg: 0.909 ± 0.281
3.256ProSer: 3.256 ± 0.439
1.969ProThr: 1.969 ± 0.4
2.65ProVal: 2.65 ± 0.51
1.287ProTrp: 1.287 ± 0.305
1.666ProTyr: 1.666 ± 0.397
0.0ProXaa: 0.0 ± 0.0
Gln
4.391GlnAla: 4.391 ± 0.818
0.454GlnCys: 0.454 ± 0.188
1.741GlnAsp: 1.741 ± 0.532
2.498GlnGlu: 2.498 ± 0.446
1.666GlnPhe: 1.666 ± 0.276
2.726GlnGly: 2.726 ± 0.473
0.909GlnHis: 0.909 ± 0.28
3.331GlnIle: 3.331 ± 0.615
2.196GlnLys: 2.196 ± 0.364
4.618GlnLeu: 4.618 ± 0.686
1.211GlnMet: 1.211 ± 0.265
1.59GlnAsn: 1.59 ± 0.368
1.136GlnPro: 1.136 ± 0.313
2.044GlnGln: 2.044 ± 0.456
1.363GlnArg: 1.363 ± 0.293
2.423GlnSer: 2.423 ± 0.383
2.65GlnThr: 2.65 ± 0.358
2.877GlnVal: 2.877 ± 0.42
0.227GlnTrp: 0.227 ± 0.108
1.59GlnTyr: 1.59 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
3.028ArgAla: 3.028 ± 0.507
0.53ArgCys: 0.53 ± 0.224
2.574ArgAsp: 2.574 ± 0.446
2.801ArgGlu: 2.801 ± 0.567
2.12ArgPhe: 2.12 ± 0.248
2.423ArgGly: 2.423 ± 0.366
0.379ArgHis: 0.379 ± 0.155
2.271ArgIle: 2.271 ± 0.434
2.953ArgLys: 2.953 ± 0.665
4.618ArgLeu: 4.618 ± 0.567
1.514ArgMet: 1.514 ± 0.386
2.574ArgAsn: 2.574 ± 0.323
1.817ArgPro: 1.817 ± 0.447
3.18ArgGln: 3.18 ± 0.545
1.969ArgArg: 1.969 ± 0.426
2.347ArgSer: 2.347 ± 0.467
2.271ArgThr: 2.271 ± 0.409
2.271ArgVal: 2.271 ± 0.387
0.833ArgTrp: 0.833 ± 0.2
1.893ArgTyr: 1.893 ± 0.34
0.0ArgXaa: 0.0 ± 0.0
Ser
5.224SerAla: 5.224 ± 0.696
0.454SerCys: 0.454 ± 0.174
5.224SerAsp: 5.224 ± 0.817
4.24SerGlu: 4.24 ± 0.696
2.044SerPhe: 2.044 ± 0.286
6.587SerGly: 6.587 ± 0.94
0.757SerHis: 0.757 ± 0.225
3.786SerIle: 3.786 ± 0.714
3.861SerLys: 3.861 ± 0.507
4.543SerLeu: 4.543 ± 0.641
1.59SerMet: 1.59 ± 0.399
4.694SerAsn: 4.694 ± 0.771
3.104SerPro: 3.104 ± 0.55
1.969SerGln: 1.969 ± 0.435
3.256SerArg: 3.256 ± 0.596
5.3SerSer: 5.3 ± 1.065
4.694SerThr: 4.694 ± 0.518
3.71SerVal: 3.71 ± 0.427
0.757SerTrp: 0.757 ± 0.295
1.817SerTyr: 1.817 ± 0.403
0.0SerXaa: 0.0 ± 0.0
Thr
5.073ThrAla: 5.073 ± 0.661
0.833ThrCys: 0.833 ± 0.296
3.71ThrAsp: 3.71 ± 0.576
4.164ThrGlu: 4.164 ± 0.454
2.801ThrPhe: 2.801 ± 0.454
4.618ThrGly: 4.618 ± 1.101
0.909ThrHis: 0.909 ± 0.36
4.088ThrIle: 4.088 ± 0.514
4.997ThrLys: 4.997 ± 0.809
5.376ThrLeu: 5.376 ± 0.742
1.666ThrMet: 1.666 ± 0.409
4.543ThrAsn: 4.543 ± 0.704
3.028ThrPro: 3.028 ± 0.635
2.801ThrGln: 2.801 ± 0.41
2.65ThrArg: 2.65 ± 0.498
4.846ThrSer: 4.846 ± 0.827
7.798ThrThr: 7.798 ± 1.932
3.71ThrVal: 3.71 ± 0.658
0.757ThrTrp: 0.757 ± 0.279
2.801ThrTyr: 2.801 ± 0.434
0.0ThrXaa: 0.0 ± 0.0
Val
5.754ValAla: 5.754 ± 0.58
0.53ValCys: 0.53 ± 0.191
3.786ValAsp: 3.786 ± 0.583
4.543ValGlu: 4.543 ± 0.549
2.574ValPhe: 2.574 ± 0.551
4.164ValGly: 4.164 ± 0.601
0.984ValHis: 0.984 ± 0.326
3.18ValIle: 3.18 ± 0.652
4.088ValLys: 4.088 ± 0.483
3.028ValLeu: 3.028 ± 0.511
1.363ValMet: 1.363 ± 0.265
3.407ValAsn: 3.407 ± 0.513
1.741ValPro: 1.741 ± 0.328
2.196ValGln: 2.196 ± 0.401
3.18ValArg: 3.18 ± 0.478
4.164ValSer: 4.164 ± 0.45
5.224ValThr: 5.224 ± 0.929
4.164ValVal: 4.164 ± 0.529
0.606ValTrp: 0.606 ± 0.198
2.271ValTyr: 2.271 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
0.909TrpAla: 0.909 ± 0.3
0.379TrpCys: 0.379 ± 0.186
0.606TrpAsp: 0.606 ± 0.204
0.984TrpGlu: 0.984 ± 0.348
0.303TrpPhe: 0.303 ± 0.117
0.227TrpGly: 0.227 ± 0.109
0.454TrpHis: 0.454 ± 0.175
0.681TrpIle: 0.681 ± 0.235
0.984TrpLys: 0.984 ± 0.357
1.514TrpLeu: 1.514 ± 0.346
0.379TrpMet: 0.379 ± 0.177
0.681TrpAsn: 0.681 ± 0.263
0.303TrpPro: 0.303 ± 0.147
0.227TrpGln: 0.227 ± 0.107
0.757TrpArg: 0.757 ± 0.235
0.909TrpSer: 0.909 ± 0.279
0.757TrpThr: 0.757 ± 0.297
0.53TrpVal: 0.53 ± 0.185
0.0TrpTrp: 0.0 ± 0.0
0.076TrpTyr: 0.076 ± 0.075
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.347TyrAla: 2.347 ± 0.443
0.454TyrCys: 0.454 ± 0.202
2.196TyrAsp: 2.196 ± 0.404
2.196TyrGlu: 2.196 ± 0.455
1.287TyrPhe: 1.287 ± 0.32
2.347TyrGly: 2.347 ± 0.348
0.757TyrHis: 0.757 ± 0.355
2.044TyrIle: 2.044 ± 0.329
2.801TyrLys: 2.801 ± 0.559
3.104TyrLeu: 3.104 ± 0.484
0.757TyrMet: 0.757 ± 0.226
1.666TyrAsn: 1.666 ± 0.369
1.06TyrPro: 1.06 ± 0.255
1.439TyrGln: 1.439 ± 0.254
2.12TyrArg: 2.12 ± 0.394
2.726TyrSer: 2.726 ± 0.49
2.12TyrThr: 2.12 ± 0.309
2.044TyrVal: 2.044 ± 0.469
0.53TyrTrp: 0.53 ± 0.19
1.363TyrTyr: 1.363 ± 0.433
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski