Acanthisitta chloris (rifleman)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091MHP0|A0A091MHP0_9PASS Uncharacterized protein (Fragment) OS=Acanthisitta chloris OX=57068 GN=N310_05782 PE=4 SV=1
VV1 pKa = 6.87YY2 pKa = 9.91EE3 pKa = 4.61HH4 pKa = 6.83QDD6 pKa = 2.99GSKK9 pKa = 10.27SLKK12 pKa = 10.85LGDD15 pKa = 4.24FGLATIVDD23 pKa = 4.14GPLYY27 pKa = 8.67TVCGTPTYY35 pKa = 10.07VAPEE39 pKa = 4.48IIAEE43 pKa = 4.08TGYY46 pKa = 10.94GLKK49 pKa = 10.19VDD51 pKa = 3.45IWAAGVITYY60 pKa = 10.31ILLCGFPPFRR70 pKa = 11.84GSGDD74 pKa = 3.61DD75 pKa = 3.63QEE77 pKa = 5.32VLFDD81 pKa = 4.35QILMGQVDD89 pKa = 4.99FPSPYY94 pKa = 8.82WDD96 pKa = 4.8NISDD100 pKa = 3.93SAKK103 pKa = 10.66EE104 pKa = 4.51LITMMLQVDD113 pKa = 3.37VDD115 pKa = 4.09LRR117 pKa = 11.84FSALQVLEE125 pKa = 4.59HH126 pKa = 6.69PWVNN130 pKa = 3.28

Molecular weight: 14.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091N7N9|A0A091N7N9_9PASS Gamma-aminobutyric acid receptor-associated protein-like 1 (Fragment) OS=Acanthisitta chloris OX=57068 GN=N310_02657 PE=3 SV=1
PP1 pKa = 7.42APVPGAVPSPVPFPVRR17 pKa = 11.84FPRR20 pKa = 11.84LSGPAPVPGAVPSPVPFPVRR40 pKa = 11.84FPRR43 pKa = 11.84LSGPAPVPGAVPSPVPFPVRR63 pKa = 11.84FPRR66 pKa = 11.84LSGPAPVPGAVPSPVPFPVRR86 pKa = 11.84FPRR89 pKa = 11.84LSGPAPVPGAVPSPVPFPVRR109 pKa = 11.84FPRR112 pKa = 11.84LSGPAPVPGAVPSPVPP128 pKa = 3.42

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9650

0

9650

4030215

30

4680

417.6

46.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.383 ± 0.019

2.291 ± 0.022

5.065 ± 0.018

7.194 ± 0.033

3.904 ± 0.02

5.932 ± 0.033

2.564 ± 0.013

4.971 ± 0.02

6.388 ± 0.028

9.709 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.011

4.086 ± 0.015

5.283 ± 0.029

4.674 ± 0.022

5.152 ± 0.02

8.203 ± 0.037

5.398 ± 0.016

6.331 ± 0.022

1.196 ± 0.008

3.014 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski