bacterium 0.1xD8-71

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8ZI62|A0A3A8ZI62_9BACT Sugar ABC transporter permease OS=bacterium 0.1xD8-71 OX=2320099 GN=D7V83_16380 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.17LKK4 pKa = 10.51KK5 pKa = 10.46VLALTLAAAMTFSLAACGGSDD26 pKa = 3.57EE27 pKa = 4.87GAAEE31 pKa = 4.32APATTQEE38 pKa = 4.22TGGSEE43 pKa = 3.82EE44 pKa = 4.09AQAPEE49 pKa = 4.09AEE51 pKa = 4.37EE52 pKa = 4.48AEE54 pKa = 4.51PEE56 pKa = 4.14AEE58 pKa = 4.18APAAAGVTYY67 pKa = 10.95ASINLGEE74 pKa = 4.71DD75 pKa = 3.56YY76 pKa = 11.16KK77 pKa = 11.65DD78 pKa = 3.08LTTTIKK84 pKa = 10.53FIHH87 pKa = 6.65HH88 pKa = 5.44KK89 pKa = 8.52TDD91 pKa = 3.41RR92 pKa = 11.84EE93 pKa = 4.03EE94 pKa = 4.86DD95 pKa = 3.25GTMASLVSKK104 pKa = 10.61FNEE107 pKa = 4.18VYY109 pKa = 10.73PNITVEE115 pKa = 4.28TEE117 pKa = 4.01GITDD121 pKa = 3.69YY122 pKa = 11.64QEE124 pKa = 5.59DD125 pKa = 3.9SLLRR129 pKa = 11.84LSTGDD134 pKa = 2.78WGDD137 pKa = 2.91IMFIPAIDD145 pKa = 3.67KK146 pKa = 11.33AEE148 pKa = 4.07LSTYY152 pKa = 8.78FIPYY156 pKa = 7.88GTVSEE161 pKa = 4.25MSGLVNFADD170 pKa = 3.07EE171 pKa = 3.95WMYY174 pKa = 11.94DD175 pKa = 3.55DD176 pKa = 3.42MCYY179 pKa = 10.64GVGYY183 pKa = 9.22MGNAQGLLYY192 pKa = 10.07NKK194 pKa = 10.03KK195 pKa = 8.82VFADD199 pKa = 3.44AGVTQLPKK207 pKa = 10.67TPDD210 pKa = 3.06EE211 pKa = 5.17FIAALQAIKK220 pKa = 10.77DD221 pKa = 3.84NTDD224 pKa = 4.57AIPLYY229 pKa = 10.26TNYY232 pKa = 10.71AAGWTMGGQWDD243 pKa = 4.2AFLGGITTGDD253 pKa = 3.91EE254 pKa = 3.36TWMNQKK260 pKa = 9.42FVHH263 pKa = 5.33TAEE266 pKa = 4.27PFKK269 pKa = 11.42DD270 pKa = 3.49NGDD273 pKa = 3.53GTGAYY278 pKa = 9.94ALYY281 pKa = 10.2KK282 pKa = 10.0ILYY285 pKa = 9.01DD286 pKa = 3.65AVEE289 pKa = 3.94QGLIEE294 pKa = 5.49DD295 pKa = 5.46DD296 pKa = 3.88YY297 pKa = 10.81TTTDD301 pKa = 3.01WEE303 pKa = 4.91GCKK306 pKa = 10.32PMINNGEE313 pKa = 4.19IGCMVLGSWAIAQMKK328 pKa = 9.56AAGDD332 pKa = 3.73NPDD335 pKa = 5.11DD336 pKa = 3.69IGYY339 pKa = 8.2MPFPITVGGKK349 pKa = 9.24QYY351 pKa = 10.3ATAGPDD357 pKa = 3.18YY358 pKa = 11.23SFGINANASEE368 pKa = 4.59DD369 pKa = 3.73NQAAAMVFVKK379 pKa = 10.09WLVEE383 pKa = 3.77EE384 pKa = 5.1SGWCDD389 pKa = 3.52FEE391 pKa = 5.99GGYY394 pKa = 9.61PISKK398 pKa = 8.26TAPTSFVFDD407 pKa = 3.29GVEE410 pKa = 3.71IVRR413 pKa = 11.84NIPALEE419 pKa = 4.19GEE421 pKa = 4.06EE422 pKa = 5.43DD423 pKa = 4.0YY424 pKa = 11.54LNEE427 pKa = 3.92MNAEE431 pKa = 4.18SEE433 pKa = 4.36LSFNAGGDD441 pKa = 3.38AKK443 pKa = 10.31VQRR446 pKa = 11.84IVEE449 pKa = 4.09AAATGAEE456 pKa = 4.24TYY458 pKa = 11.1DD459 pKa = 5.58DD460 pKa = 5.71IMADD464 pKa = 3.09WTAAWNAAQEE474 pKa = 4.31EE475 pKa = 4.97CGVEE479 pKa = 3.91VLYY482 pKa = 11.07

Molecular weight: 52.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9A4F8|A0A3A9A4F8_9BACT Thioredoxin OS=bacterium 0.1xD8-71 OX=2320099 GN=D7V83_10870 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3809

0

3809

1197674

25

2354

314.4

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.455 ± 0.045

1.55 ± 0.013

5.714 ± 0.031

7.87 ± 0.039

4.025 ± 0.029

7.08 ± 0.036

1.76 ± 0.015

7.195 ± 0.037

6.205 ± 0.037

8.982 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.274 ± 0.018

4.147 ± 0.024

3.146 ± 0.02

3.551 ± 0.026

4.861 ± 0.03

5.53 ± 0.032

5.17 ± 0.029

6.982 ± 0.033

1.008 ± 0.014

4.493 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski