Planctomycetes bacterium K22_7

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rubripirellula

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6373 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517NAA3|A0A517NAA3_9BACT Uncharacterized protein OS=Planctomycetes bacterium K22_7 OX=1930273 GN=K227x_24480 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.36RR3 pKa = 11.84STRR6 pKa = 11.84RR7 pKa = 11.84TKK9 pKa = 9.56NQRR12 pKa = 11.84TSLEE16 pKa = 4.05TLEE19 pKa = 5.46ARR21 pKa = 11.84DD22 pKa = 3.6LLAAFGTPWPEE33 pKa = 3.75ARR35 pKa = 11.84DD36 pKa = 3.63LSISFPADD44 pKa = 2.91EE45 pKa = 4.37VAIGEE50 pKa = 4.33YY51 pKa = 10.67EE52 pKa = 3.76NDD54 pKa = 2.79IRR56 pKa = 11.84QTLDD60 pKa = 4.06AADD63 pKa = 3.56QQQWQEE69 pKa = 3.82LALRR73 pKa = 11.84AYY75 pKa = 7.25QTWAIHH81 pKa = 6.41ADD83 pKa = 3.17INVGLRR89 pKa = 11.84NDD91 pKa = 3.5FDD93 pKa = 4.39VSFGAAGLMNQDD105 pKa = 2.86PRR107 pKa = 11.84FGEE110 pKa = 3.94FRR112 pKa = 11.84IGAFPQDD119 pKa = 2.93GLMANSLPFQVAAGTYY135 pKa = 10.38SGDD138 pKa = 3.59LLLNSNQDD146 pKa = 3.04YY147 pKa = 10.97RR148 pKa = 11.84LYY150 pKa = 10.87DD151 pKa = 3.39WPDD154 pKa = 3.01SVAPDD159 pKa = 4.1LSTWDD164 pKa = 3.19EE165 pKa = 4.06GQRR168 pKa = 11.84DD169 pKa = 4.16LFSVLLHH176 pKa = 6.14EE177 pKa = 5.52AGNTLGVDD185 pKa = 4.04DD186 pKa = 5.75NQMEE190 pKa = 4.32WSVMFGQYY198 pKa = 6.98TTPKK202 pKa = 10.19GVLAPEE208 pKa = 5.15DD209 pKa = 3.25IGEE212 pKa = 4.05IQQLYY217 pKa = 9.99GMRR220 pKa = 11.84SDD222 pKa = 4.08PYY224 pKa = 10.84EE225 pKa = 4.26SVTNDD230 pKa = 3.23QLATATIIPIPIGFAPASEE249 pKa = 4.63TIRR252 pKa = 11.84TRR254 pKa = 11.84GSIASANDD262 pKa = 2.76VDD264 pKa = 4.4HH265 pKa = 7.06FRR267 pKa = 11.84FSPVPGSNSVTIRR280 pKa = 11.84LNAEE284 pKa = 4.46GISLLEE290 pKa = 4.03SQIEE294 pKa = 4.18VTLADD299 pKa = 3.81GTVVAQSMAASVFEE313 pKa = 4.4NDD315 pKa = 3.52HH316 pKa = 5.27QVEE319 pKa = 4.3LTGLGVGSDD328 pKa = 3.79LFVKK332 pKa = 8.97VTAADD337 pKa = 3.58SDD339 pKa = 4.11SLYY342 pKa = 11.03SIGDD346 pKa = 3.58YY347 pKa = 10.88VLEE350 pKa = 4.32LDD352 pKa = 4.0YY353 pKa = 11.31RR354 pKa = 11.84DD355 pKa = 4.59AAVQSQDD362 pKa = 3.6IVPGDD367 pKa = 3.89YY368 pKa = 10.55QSGADD373 pKa = 3.58SLYY376 pKa = 11.11SGFDD380 pKa = 3.69LLDD383 pKa = 4.14LEE385 pKa = 4.97TDD387 pKa = 3.46VSTTASAIEE396 pKa = 4.08IDD398 pKa = 3.45AHH400 pKa = 6.91DD401 pKa = 4.98SIDD404 pKa = 3.57EE405 pKa = 4.07RR406 pKa = 11.84FEE408 pKa = 3.94IQSSVGSASDD418 pKa = 3.32VDD420 pKa = 3.69VWKK423 pKa = 10.93VVAPSTSQQRR433 pKa = 11.84LIVSLAGVGSGSPDD447 pKa = 3.32LDD449 pKa = 3.92LYY451 pKa = 11.62VVDD454 pKa = 5.57DD455 pKa = 3.82QGQSVGTTARR465 pKa = 11.84LRR467 pKa = 11.84PDD469 pKa = 3.39GTWTVEE475 pKa = 4.08VNQPTAGQEE484 pKa = 3.66YY485 pKa = 9.09FIRR488 pKa = 11.84VSVDD492 pKa = 2.71PGSAVEE498 pKa = 4.02VGNYY502 pKa = 7.96VAVAEE507 pKa = 4.74FVSPPAQMNDD517 pKa = 4.18LISGDD522 pKa = 3.13VDD524 pKa = 3.39ATVDD528 pKa = 3.66EE529 pKa = 4.94FFRR532 pKa = 11.84WTASKK537 pKa = 10.99SKK539 pKa = 10.55LFRR542 pKa = 11.84FDD544 pKa = 4.14FSAFGAAAGDD554 pKa = 4.0GARR557 pKa = 11.84LRR559 pKa = 11.84IYY561 pKa = 10.07DD562 pKa = 3.54AHH564 pKa = 4.84THH566 pKa = 6.3EE567 pKa = 4.44IRR569 pKa = 11.84LVIGAQNDD577 pKa = 3.46MTRR580 pKa = 11.84TGMAWLQQGDD590 pKa = 3.9YY591 pKa = 10.53VLRR594 pKa = 11.84FTAFSADD601 pKa = 3.92DD602 pKa = 3.54QSVAPIQFTLTCDD615 pKa = 4.86GISDD619 pKa = 4.07DD620 pKa = 4.67QDD622 pKa = 3.27EE623 pKa = 4.62TGYY626 pKa = 11.3DD627 pKa = 3.73PDD629 pKa = 4.71NDD631 pKa = 4.32PDD633 pKa = 4.08YY634 pKa = 11.59DD635 pKa = 4.14PYY637 pKa = 10.4TYY639 pKa = 10.32EE640 pKa = 4.03YY641 pKa = 9.5TYY643 pKa = 11.08HH644 pKa = 7.79DD645 pKa = 3.45YY646 pKa = 11.41GYY648 pKa = 10.44YY649 pKa = 8.71YY650 pKa = 11.13SNYY653 pKa = 9.22EE654 pKa = 3.89YY655 pKa = 11.06YY656 pKa = 10.8YY657 pKa = 11.01DD658 pKa = 3.2WDD660 pKa = 3.37YY661 pKa = 11.68YY662 pKa = 9.22YY663 pKa = 10.84QYY665 pKa = 11.45DD666 pKa = 3.66GDD668 pKa = 4.23PNYY671 pKa = 11.21AHH673 pKa = 7.04YY674 pKa = 11.19YY675 pKa = 7.98EE676 pKa = 4.61

Molecular weight: 74.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517NIX7|A0A517NIX7_9BACT Copper-sensing transcriptional repressor CsoR OS=Planctomycetes bacterium K22_7 OX=1930273 GN=csoR_2 PE=4 SV=1
MM1 pKa = 7.41GMTVLSGLGQSAIRR15 pKa = 11.84SVMGMTVHH23 pKa = 6.78SGLGQSTIRR32 pKa = 11.84SVMGMTVHH40 pKa = 6.9SGLGQSAIRR49 pKa = 11.84SVMGMTGLSALGQSAIRR66 pKa = 11.84LVMGMTVHH74 pKa = 6.71SGLGQSTIRR83 pKa = 11.84LVMGMTVHH91 pKa = 6.71SGLGQSTIRR100 pKa = 11.84SVMGMTVHH108 pKa = 6.9SGLGQSAIRR117 pKa = 11.84SVMGMTVHH125 pKa = 6.78SGLGQSTIRR134 pKa = 11.84SVMGMTVHH142 pKa = 6.9SGLGQSAIRR151 pKa = 11.84SVMGMTVHH159 pKa = 6.78SGLGQSTIRR168 pKa = 11.84SVMGMTVHH176 pKa = 6.9SGLGQSAIRR185 pKa = 11.84SVMGMTVHH193 pKa = 6.9SGLGQSAIRR202 pKa = 11.84LVMGMTVLSGLGQSAIRR219 pKa = 11.84SVMGMTVHH227 pKa = 6.9SGLGQSAIRR236 pKa = 11.84LVMGMTVHH244 pKa = 6.83SGLGQSAIRR253 pKa = 11.84SVMGMTVHH261 pKa = 6.78SGLGQSTIRR270 pKa = 11.84SVMGMTVHH278 pKa = 6.9SGLGQSAIRR287 pKa = 11.84SVMGMTVHH295 pKa = 6.78SGLGQSTIRR304 pKa = 11.84SVMGMTVHH312 pKa = 6.78SGLGQSTIRR321 pKa = 11.84SVMGMTGLSALGQSAIRR338 pKa = 11.84SVMGMTVLSVLGQSTIRR355 pKa = 11.84LVVGMTVLSVLGQSTIRR372 pKa = 11.84LVVRR376 pKa = 11.84FATRR380 pKa = 11.84LAGSQDD386 pKa = 3.42VQRR389 pKa = 11.84RR390 pKa = 11.84AASCVCKK397 pKa = 10.73SKK399 pKa = 10.69INCPSSVMAAVSRR412 pKa = 11.84INAASLVGTWRR423 pKa = 11.84ILCAASRR430 pKa = 11.84AVGIQRR436 pKa = 3.87

Molecular weight: 44.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6373

0

6373

2431915

29

11647

381.6

41.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.91 ± 0.034

1.094 ± 0.015

6.858 ± 0.039

5.305 ± 0.027

3.597 ± 0.017

7.896 ± 0.044

2.19 ± 0.019

5.214 ± 0.023

3.304 ± 0.027

9.099 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.02

3.155 ± 0.027

5.285 ± 0.028

4.189 ± 0.022

6.652 ± 0.042

7.058 ± 0.029

5.797 ± 0.044

7.268 ± 0.029

1.494 ± 0.017

2.259 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski