Streptomyces tsukubensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6478 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4A5C4|A0A1V4A5C4_9ACTN Uncharacterized protein OS=Streptomyces tsukubensis OX=83656 GN=B1H18_23075 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84IHH4 pKa = 6.14RR5 pKa = 11.84TIAATATLALTLGTAALVAPSAQAAGATATVVHH38 pKa = 7.02EE39 pKa = 5.44DD40 pKa = 2.95GKK42 pKa = 10.84LWYY45 pKa = 9.65KK46 pKa = 10.42AAPGQQNQLTVDD58 pKa = 3.6EE59 pKa = 5.21EE60 pKa = 4.19IEE62 pKa = 3.87QRR64 pKa = 11.84GEE66 pKa = 3.79FEE68 pKa = 4.0SYY70 pKa = 10.87YY71 pKa = 11.21VLTFHH76 pKa = 7.4DD77 pKa = 5.29RR78 pKa = 11.84YY79 pKa = 11.07DD80 pKa = 3.49IDD82 pKa = 4.03IDD84 pKa = 3.81ATSATWDD91 pKa = 3.3EE92 pKa = 4.55CVYY95 pKa = 8.89PTQGDD100 pKa = 3.72HH101 pKa = 6.54GVVRR105 pKa = 11.84CAVEE109 pKa = 4.14IPQNSDD115 pKa = 3.28DD116 pKa = 4.14SDD118 pKa = 4.34SYY120 pKa = 11.81DD121 pKa = 3.49IDD123 pKa = 5.79LGDD126 pKa = 4.48EE127 pKa = 4.76DD128 pKa = 4.88DD129 pKa = 4.21TATLEE134 pKa = 4.36PDD136 pKa = 2.81SDD138 pKa = 3.24AWAGVHH144 pKa = 6.6GGPGDD149 pKa = 4.09DD150 pKa = 3.71VLKK153 pKa = 10.94GSASSILHH161 pKa = 6.68GDD163 pKa = 4.19DD164 pKa = 5.23GDD166 pKa = 4.44DD167 pKa = 3.82SLDD170 pKa = 3.75GGGGPFGFGSYY181 pKa = 10.23GGPGNDD187 pKa = 3.48TLTHH191 pKa = 6.73CGQDD195 pKa = 3.55CFGEE199 pKa = 4.4AGNDD203 pKa = 3.48SLTGTDD209 pKa = 4.15EE210 pKa = 4.01EE211 pKa = 4.92NNLHH215 pKa = 6.98GDD217 pKa = 3.74DD218 pKa = 5.15GDD220 pKa = 5.9DD221 pKa = 3.42ILHH224 pKa = 6.0GQGGADD230 pKa = 3.24VLYY233 pKa = 10.49GGKK236 pKa = 10.43GNDD239 pKa = 3.36MLYY242 pKa = 11.19GEE244 pKa = 5.53AGDD247 pKa = 3.76DD248 pKa = 3.96TLWGNSGDD256 pKa = 3.78DD257 pKa = 4.07VLWGGPGTDD266 pKa = 3.97ALSGGAGTNEE276 pKa = 3.85LHH278 pKa = 6.82QDD280 pKa = 3.04

Molecular weight: 29.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4AAE9|A0A1V4AAE9_9ACTN N-acetyltransferase domain-containing protein OS=Streptomyces tsukubensis OX=83656 GN=B1H18_13300 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6478

0

6478

2226907

18

24465

343.8

36.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.252 ± 0.041

0.73 ± 0.013

6.035 ± 0.027

5.804 ± 0.031

2.706 ± 0.018

9.757 ± 0.033

2.354 ± 0.016

3.097 ± 0.029

2.263 ± 0.028

10.095 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.753 ± 0.015

1.744 ± 0.016

6.164 ± 0.046

2.693 ± 0.02

8.016 ± 0.032

5.471 ± 0.025

6.208 ± 0.025

8.324 ± 0.036

1.474 ± 0.014

2.061 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski