Streptococcus satellite phage Javan322

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJM6|A0A4D5ZJM6_9VIRU Helicase loader OS=Streptococcus satellite phage Javan322 OX=2558635 GN=JavanS322_0014 PE=4 SV=1
MM1 pKa = 7.69NYY3 pKa = 10.18AQDD6 pKa = 3.98YY7 pKa = 10.69LDD9 pKa = 5.6DD10 pKa = 3.83ILVRR14 pKa = 11.84MAYY17 pKa = 9.61HH18 pKa = 6.36SSGIEE23 pKa = 3.94GNTISLPEE31 pKa = 4.05TVSIILEE38 pKa = 4.11STLPRR43 pKa = 11.84NGKK46 pKa = 8.92SICEE50 pKa = 3.92FYY52 pKa = 10.77EE53 pKa = 4.24IEE55 pKa = 3.96NHH57 pKa = 5.78KK58 pKa = 10.51QAFSYY63 pKa = 11.15LLDD66 pKa = 3.75SLANNQALTVGLVQDD81 pKa = 4.4FHH83 pKa = 8.52ALLVDD88 pKa = 4.23RR89 pKa = 11.84LQHH92 pKa = 7.12DD93 pKa = 3.94RR94 pKa = 11.84GQFKK98 pKa = 10.05QVQNAIIGAEE108 pKa = 4.04FQTASPAEE116 pKa = 4.3TPYY119 pKa = 11.72LMTQWADD126 pKa = 3.11NTAYY130 pKa = 10.89RR131 pKa = 11.84LDD133 pKa = 3.53HH134 pKa = 6.29AQNEE138 pKa = 4.42KK139 pKa = 10.83EE140 pKa = 4.01ILEE143 pKa = 4.27ILADD147 pKa = 3.24THH149 pKa = 6.32IQIEE153 pKa = 4.59RR154 pKa = 11.84IHH156 pKa = 6.6PFSDD160 pKa = 3.38GNGRR164 pKa = 11.84TGRR167 pKa = 11.84LLLMYY172 pKa = 10.74LAMKK176 pKa = 9.42YY177 pKa = 10.35LNAPIIVSKK186 pKa = 10.58DD187 pKa = 3.1SRR189 pKa = 11.84AHH191 pKa = 5.47YY192 pKa = 9.93MEE194 pKa = 5.78LLANQDD200 pKa = 3.4VTGLADD206 pKa = 4.16LFKK209 pKa = 11.06EE210 pKa = 4.12SLDD213 pKa = 3.74YY214 pKa = 9.17EE215 pKa = 4.62TTRR218 pKa = 11.84KK219 pKa = 9.88GQFF222 pKa = 2.95

Molecular weight: 25.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPT0|A0A4D5ZPT0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan322 OX=2558635 GN=JavanS322_0018 PE=4 SV=1
MM1 pKa = 7.86LITSTQAKK9 pKa = 9.74AIRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.66QADD17 pKa = 3.4KK18 pKa = 11.27KK19 pKa = 10.16LTAKK23 pKa = 10.18QAGEE27 pKa = 4.41EE28 pKa = 3.96IGVTQVTYY36 pKa = 10.88RR37 pKa = 11.84KK38 pKa = 9.3IRR40 pKa = 11.84DD41 pKa = 3.51GGEE44 pKa = 4.09VKK46 pKa = 10.52PSIYY50 pKa = 9.91QKK52 pKa = 11.14AMQWLAEE59 pKa = 4.48DD60 pKa = 4.07YY61 pKa = 11.23

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3258

43

388

155.1

17.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.218 ± 0.473

0.645 ± 0.099

5.985 ± 0.407

8.257 ± 0.883

4.359 ± 0.355

4.389 ± 0.437

1.78 ± 0.352

7.244 ± 0.338

10.405 ± 0.697

9.791 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.283

6.016 ± 0.615

2.149 ± 0.216

4.236 ± 0.355

4.543 ± 0.366

6.2 ± 0.519

6.63 ± 0.526

4.972 ± 0.457

0.706 ± 0.082

4.512 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski