Mucilaginibacter psychrotolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5520 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8SE64|A0A4Y8SE64_9SPHI DoxX family protein OS=Mucilaginibacter psychrotolerans OX=1524096 GN=E2R66_14150 PE=4 SV=1
MM1 pKa = 7.65FDD3 pKa = 5.22DD4 pKa = 6.21DD5 pKa = 6.15DD6 pKa = 4.72WDD8 pKa = 5.69DD9 pKa = 5.82DD10 pKa = 5.43DD11 pKa = 7.5DD12 pKa = 5.74DD13 pKa = 6.71LEE15 pKa = 6.39DD16 pKa = 3.78MLDD19 pKa = 3.91GEE21 pKa = 4.77EE22 pKa = 4.05ATNLQMLNLIKK33 pKa = 9.9KK34 pKa = 7.71TNEE37 pKa = 3.82TYY39 pKa = 10.73KK40 pKa = 10.74EE41 pKa = 4.19FIGNGYY47 pKa = 9.72QDD49 pKa = 4.14GNPVAGQPIGTGYY62 pKa = 8.69TLVDD66 pKa = 3.45KK67 pKa = 11.1EE68 pKa = 4.42EE69 pKa = 4.0NTLYY73 pKa = 9.69EE74 pKa = 4.25TFDD77 pKa = 3.54NDD79 pKa = 4.12AEE81 pKa = 4.16EE82 pKa = 5.9AEE84 pKa = 4.41FNLLKK89 pKa = 10.73QLVFDD94 pKa = 4.36DD95 pKa = 3.98QNYY98 pKa = 8.64EE99 pKa = 3.85QAIPQLLQAIEE110 pKa = 4.8KK111 pKa = 9.75YY112 pKa = 8.42PQRR115 pKa = 11.84EE116 pKa = 3.49QFYY119 pKa = 10.57FLLQFAYY126 pKa = 8.94THH128 pKa = 6.41NNQFDD133 pKa = 3.76EE134 pKa = 4.76SDD136 pKa = 3.83VILEE140 pKa = 3.86RR141 pKa = 11.84TYY143 pKa = 11.71NRR145 pKa = 11.84FPDD148 pKa = 4.14YY149 pKa = 10.73LPHH152 pKa = 6.93RR153 pKa = 11.84VDD155 pKa = 4.56FADD158 pKa = 3.31ILLDD162 pKa = 3.27QGRR165 pKa = 11.84NDD167 pKa = 4.19EE168 pKa = 4.33IPAVFDD174 pKa = 3.9NKK176 pKa = 10.97ADD178 pKa = 3.91LADD181 pKa = 4.19IYY183 pKa = 10.73PGQTEE188 pKa = 4.16FNIEE192 pKa = 3.77LAANYY197 pKa = 9.28YY198 pKa = 11.01GIMCRR203 pKa = 11.84YY204 pKa = 8.8YY205 pKa = 10.91SIIGDD210 pKa = 3.4IASADD215 pKa = 3.75RR216 pKa = 11.84YY217 pKa = 9.74MDD219 pKa = 3.82ALINEE224 pKa = 4.98DD225 pKa = 3.9YY226 pKa = 11.54NLITQYY232 pKa = 9.99GTTLLKK238 pKa = 10.4IPVYY242 pKa = 10.88GFLTAKK248 pKa = 10.2FEE250 pKa = 4.38AMNTGDD256 pKa = 3.77EE257 pKa = 4.23ALDD260 pKa = 3.77FF261 pKa = 4.86

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8SN46|A0A4Y8SN46_9SPHI TonB-dependent receptor OS=Mucilaginibacter psychrotolerans OX=1524096 GN=E2R66_02225 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.42KK3 pKa = 9.71ILLSLAITISAIVAQAQTPIVVRR26 pKa = 11.84HH27 pKa = 4.71PRR29 pKa = 11.84QRR31 pKa = 11.84VVVLTRR37 pKa = 11.84PAVARR42 pKa = 11.84PVLVTRR48 pKa = 11.84PLIVRR53 pKa = 11.84PVRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84VVIVHH63 pKa = 6.71

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5520

0

5520

1844079

25

3573

334.1

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.094 ± 0.034

0.803 ± 0.01

5.488 ± 0.023

5.229 ± 0.037

4.868 ± 0.024

6.926 ± 0.033

1.879 ± 0.017

7.057 ± 0.029

6.878 ± 0.024

9.54 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.016

5.475 ± 0.034

3.976 ± 0.019

3.728 ± 0.02

3.936 ± 0.022

6.048 ± 0.03

5.918 ± 0.036

6.433 ± 0.025

1.203 ± 0.013

4.247 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski